A novel reporter for helicase activity in translation uncovers DDX3X interactions
Kevin C. Wilkins,
Till Schroeder,
Sohyun Gu
et al.
Abstract:DDX3X regulates the translation of a subset of human transcripts containing complex 5′ untranslated regions (5′ UTRs). In this study we developed the helicase activity reporter for translation (HART) which uses DDX3X-sensitive 5′ UTRs to measure DDX3X mediated translational activity in cells. To directly measure RNA structure in DDX3X dependent mRNAs, we used SHAPE-MaP to determine the secondary structures present in DDX3X-sensitive 5′ UTRs and then employed HART to investigate how sequence alterations influen… Show more
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