A novel real‐time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses

Katano, Harutaka; Kano, Motofumi; Nakamura, T.; Kuriyama, Takayuki; Asanuma, Hideki; Sata, Tetsutaro

doi:10.1002/jmv.21962

Cited by 52 publications

(49 citation statements)

References 24 publications

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“…Case reports and clinical studies from Europe have also raised suspicions about serologic cross-reactivity in the presence of other viral infections, such as Epstein-Barr virus (EBV) or cytomegalovirus (CMV) (201,202). Several recent serologic assays and other diagnostic methods have been found to have improved performance in a wide range of settings (194,198,(203)(204)(205)(206)(207).…”

Section: Scope and Duration Of Protective Immunity To Hev Following Imentioning

confidence: 99%

Host Immune Status and Response to Hepatitis E Virus Infection

2014

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SUMMARY Hepatitis E virus (HEV), identified over 30 years ago, remains a serious threat to life, health, and productivity in developing countries where access to clean water is limited. Recognition that HEV also circulates as a zoonotic and food-borne pathogen in developed countries is more recent. Even without treatment, most cases of HEV-related acute viral hepatitis (with or without jaundice) resolve within 1 to 2 months. However, HEV sometimes leads to acute liver failure, chronic infection, or extrahepatic symptoms. The mechanisms of pathogenesis appear to be substantially immune mediated. This review covers the epidemiology of HEV infection worldwide, the humoral and cellular immune responses to HEV, and the persistence and protection of antibodies produced in response to both natural infection and vaccines. We focus on the contributions of altered immune states (associated with pregnancy, human immunodeficiency virus [HIV], and immunosuppressive agents used in cancer and transplant medicine) to the elevated risks of chronic infection (in immunosuppressed/immunocompromised patients) and acute liver failure and mortality (among pregnant women). We conclude by discussing outstanding questions about the immune response to HEV and interactions with hormones and comorbid conditions. These questions take on heightened importance now that a vaccine is available.

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Section: Scope and Duration Of Protective Immunity To Hev Following Imentioning

confidence: 99%

Host Immune Status and Response to Hepatitis E Virus Infection

2014

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“…At the same time, anti-myocardial antibody testing was positive. In accordance with the statement of European Society of Cardiology (ESC) Working Group on Myocardial and Pericardial Diseases, 4) oral administration of prednisolone was started at an initial dose of 0.5 mg/kg once daily on day 106 after ruling out active viral infection by multivirus real-time polymerase chain reaction (PCR) using the specimen 5) obtained from the fourth EMB (day 69). Prednisolone was gradually reduced and then withdrawn 3 months after initiation.…”

Section: Case Reportmentioning

confidence: 99%

Fulminant Myocarditis With Prolonged Active Lymphocytic Infiltration After Hemodynamic Recovery

et al. 2017

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SummaryFulminant myocarditis is a highly mortal syndrome. Meanwhile, the clinical course in surviving patients is generally self-limiting. This is a rare case of fulminant myocarditis with prolonged lymphocytic infiltration after hemodynamic recovery. A 64-year-old man was diagnosed with fulminant myocarditis and required intensive care with veno-arterial extracorporeal membrane oxygenation. Left ventricular function gradually improved but complete atrioventricular block (CAVB) persisted. Follow-up endomyocardial biopsies (EMBs) showed prolonged active infiltration of lymphocytes along with Meanwhile, the clinical course of fulminant myocarditis in surviving patients is self-limiting and the long-term prognosis is favorable.2) Therefore, there are few individual case reports of fulminant myocarditis with prolonged lymphocytic infiltration after hemodynamic recovery. Case ReportA 64-year-old man complained of chest discomfort and low-grade fever for two days, and was admitted to a domestic hospital with suspected acute myocarditis. A 12-lead electrocardiogram showed complete atrioventricular block (CAVB) with ST elevation in the inferior leads, and echocardiography showed reduced left ventricular ejection fraction (LVEF) without LV dilation. Serum creatine kinase was elevated to 512 IU/ L, and qualitative troponin-T test was positive. Emergent coronary angiography revealed no significant lesions in the coronary arteries. He was diagnosed with acute heart failure caused by non-ischemic heart disease, and continuous infusion of carperitide and dobutamine was started. On the next day, he suddenly developed ventricular fibrillation and was immediately defibrillated. He was then transferred to our hospital after insertion of a temporary transvenous pacemaker (day 1). On arrival, he collapsed into a cardiogenic shock-state so veno-arterial extracorporeal membrane oxygenation (ECMO) and intra-aortic balloon pumping (IABP) were immediately started ( Figure 1A). An endomyocardial biopsy (EMB) from the right ventricular septum was performed, and the biopsy specimens showed massive lymphocytic infiltration associated with myocytolysis without obvious myocardial fibrosis ( Figure 1B and C). Strong immunostaining for tenascin-C, a marker of active inflammation,3) was seen around cardiomyocytes ( Figure 1D). His troponin-I level was 31.3 ng/mL. The serum viral titers were not increased. The LVEF worsened, falling to 15% on day 3, but his cardiac function gradually recovered and he was successfully weaned off the ECMO and IABP on days 7 and 9, respectively, despite persistent CAVB with junctional escape rhythm. The temporary pacemaker lead was removed because of pacing failure on day 8 with his intrinsic heart rate of 70 bpm, and then he suddenly developed ventricular fibrillation with successful resuscitation on day 14. A temporary pacemaker was immediately re-inserted and a second EMB was performed. The specimens still showed abundant lymphocyte infiltration and myocytolysis. Life-threatening arrhythmias did not occur subsequ...

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“…Multi-virus realtime PCR was performed as previously reported (12). Briefly, each probe-primer set was prepared in 96-well plates and the extracted DNA and RNA were mixed in each well, followed by a PCR reaction (QuantiTect Multiplex One-step real-time RT-PCR kit, Qiagen, Tokyo, Japan) and detection of the quantity of viral genome (Stratagene MX 3005P, Agilent Technologies, Santa Clara, USA).…”

Section: Multi-virus Real-time Pcrmentioning

confidence: 99%

Knowledge Obtained from an Elderly Case of Japanese Encephalitis

et al. 2016

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The nationwide introduction of a Japanese encephalitis (JE) vaccine has contributed to a reduction in the annual infection rate of JE in Japan. However, the current neutralizing antibody prevalence ratio in Japan is approximately 20% in children 3-4 years of age and in people in their forties and fifties. We herein report a man with JE who was definitively diagnosed by multi-virus real-time polymerase chain reaction employing biopsied brain tissue and serological examinations. JE should be kept in mind when a patient has severe encephalitis of unknown etiology. In order to protect the susceptible population from JE, vaccination is recommended, especially for children and middle-aged people.

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A novel real‐time PCR system for simultaneous detection of human viruses in clinical samples from patients with uncertain diagnoses

Cited by 52 publications

References 24 publications

Host Immune Status and Response to Hepatitis E Virus Infection

Host Immune Status and Response to Hepatitis E Virus Infection

Fulminant Myocarditis With Prolonged Active Lymphocytic Infiltration After Hemodynamic Recovery

Knowledge Obtained from an Elderly Case of Japanese Encephalitis

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