A Novel Rat Discitis Model Using Bioluminescent Staphylococcus Aureus

Bostian, Phillip A.; Karnes, Jonathan; Cui, Shari; Emery, Sanford E.

doi:10.1016/j.spinee.2016.07.189

Cited by 2 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These studies used in vivo monitoring of bioluminescent bacteria, histological evaluation, bacterial cultures, biofilm analysis and postmortem radiographic imaging. In the model of isolated discitis, radiographic analysis revealed reduced IVD height, evidence of osteophyte formation and discitis, while destruction of the IVD and vertebral endplates was observed histologically 34 . A significant osseous infection was confirmed in implant‐inoculated model with localized tissue destruction and loss of bone; soft tissue was filled with young granulation tissue characterized by infiltration of inflammatory cells.…”

Section: Models Of Spinal Infectionsmentioning

confidence: 94%

Section: Models Of Spinal Infectionsmentioning

confidence: 99%

“…Several rat models of infection have included lumbar vertebral osteomyelitis, implant‐associated infection, isolated discitis and external fixation colonization characterization 33‐35 33,34,36 . Titanium screws inoculated with S. aureus have been used to reproduce an implant associated infection to investigate the efficacy of antibiotic therapies 33,36 .…”

Section: Models Of Spinal Infectionsmentioning

confidence: 99%

“…33-35 10 2 -10 6 CFU of S. aureus was used as an inoculating agent in infectious models. 33,34,36 Titanium screws inoculated with S. aureus have been used to reproduce an implant associated infection to investigate the efficacy of antibiotic therapies. 33,36 In the study of pin tract bacterial migration, skin flora colonized the titanium pins in the external fixation model.…”

Section: Rodent Modelsmentioning

confidence: 99%

See 3 more Smart Citations

Preclinical models of vertebral osteomyelitis and associated infections: Current models and recommendations for study design

2021

View full text Add to dashboard Cite

Spine-related infections, such as vertebral osteomyelitis, discitis, or spondylitis, are rare diseases that mostly affect adults, and are usually of hematogenous origin. The incidence of this condition has gradually risen in recent years because of increases in spine-related surgery and hospital-acquired infections, an aging population, and intravenous (IV) drug use. Spine infections are most commonly caused by Staphylococcus aureus, while other systemic infections such as tuberculosis and brucellosis can also cause spondylitis. Various animal models of vertebral osteomyelitis and associated infections have been investigated in mouse, rat, chicken, rabbit, dog, and sheep models by hematogenous and direct inoculation in surgery, each with their strengths and limitations. This review is the first of its kind to concisely analyze the various existing animal models used to reproduce clinically relevant models of infection. Spine-related infection models must address the unique anatomy of the spine, the avascular nature of its structures and tissues and the consequences of tissue destruction such as spinal cord compression. Further investigation is necessary to elucidate the specific mechanisms of host-microbe response to inform antimicrobial therapy and administration techniques in a technically demanding body cavity. Small-animal models are not suitable for large instrumentation, and difficult IV access thwarts antibiotic administration. In contrast, large-animal models can be implanted with clinically relevant instrumentation and are resilient to repeat procedures to study postoperative infection. A canine model of infection offers a unique opportunity to design and investigate antimicrobial treatments through recruitment a rich population of canine patients, presenting with a natural disease that is suitable for randomized trials.

show abstract

Section: Models Of Spinal Infectionsmentioning

confidence: 94%

Section: Models Of Spinal Infectionsmentioning

confidence: 99%

Section: Models Of Spinal Infectionsmentioning

confidence: 99%

Section: Rodent Modelsmentioning

confidence: 99%

See 2 more Smart Citations

Preclinical models of vertebral osteomyelitis and associated infections: Current models and recommendations for study design

2021

View full text Add to dashboard Cite

show abstract

“…Statistical analysis. According to prior rat PJI studies, [37][38][39][40][41][42] we chose an appropriate sample size to reduce Type 1 errors using the G-power program (version 3.1; Germany; serum α1-AGP levels, n = 6; serum vancomycin levels, n = 6; microbiological analysis of joint tissue, n = 7; micro-CT analysis of the knee joint, n = 6; SEM of the cement spacer, n = 6; histopathological analysis of the knee, n = 6). Statistical analyses were performed using SPSS software (version 22.0; IBM, USA) and are presented as the means and standard error of the means (SEM).…”

Section: Methodsmentioning

confidence: 99%

Intra-articular versus systemic vancomycin for the treatment of periprosthetic joint infection after debridement and spacer implantation in a rat model

Wei

Tong

Wang

et al. 2022

Bone & Joint Research

View full text Add to dashboard Cite

Aims Treatment outcomes for methicillin-resistant Staphylococcus aureus (MRSA) periprosthetic joint infection (PJI) using systemic vancomycin and antibacterial cement spacers during two-stage revision arthroplasty remain unsatisfactory. This study explored the efficacy and safety of intra-articular vancomycin injections for PJI control after debridement and cement spacer implantation in a rat model. Methods Total knee arthroplasty (TKA), MRSA inoculation, debridement, and vancomycin-spacer implantation were performed successively in rats to mimic first-stage PJI during the two-stage revision arthroplasty procedure. Vancomycin was administered intraperitoneally or intra-articularly for two weeks to control the infection after debridement and spacer implantation. Results Rats receiving intra-articular vancomycin showed the best outcomes among the four treatment groups, with negative bacterial cultures, increased weight gain, increased capacity for weightbearing activities, increased residual bone volume preservation, and reduced inflammatory reactions in the joint tissues, indicating MRSA eradication in the knee. The vancomycin-spacer and/or systemic vancomycin failed to eliminate the MRSA infections following a two-week antibiotic course. Serum vancomycin levels did not reach nephrotoxic levels in any group. Mild renal histopathological changes, without changes in serum creatinine levels, were observed in the intraperitoneal vancomycin group compared with the intra-articular vancomycin group, but no changes in hepatic structure or serum alanine aminotransferase or aspartate aminotransferase levels were observed. No local complications were observed, such as sinus tract or non-healing surgical incisions. Conclusion Intra-articular vancomycin injection was effective and safe for PJI control following debridement and spacer implantation in a rat model during two-stage revision arthroplasties, with better outcomes than systemic vancomycin administration. Cite this article: Bone Joint Res 2022;11(6):371–385.

show abstract

A Novel Rat Discitis Model Using Bioluminescent Staphylococcus Aureus

Cited by 2 publications

References 0 publications

Preclinical models of vertebral osteomyelitis and associated infections: Current models and recommendations for study design

Preclinical models of vertebral osteomyelitis and associated infections: Current models and recommendations for study design

Intra-articular versus systemic vancomycin for the treatment of periprosthetic joint infection after debridement and spacer implantation in a rat model

Contact Info

Product

Resources

About