A novel, rapid and simple method for detecting brucellosis based on rapid vertical flow technology

Shi, Feng; Sun, Yixiao; Wu, Youzhi; Zhu, Mingsong; Feng, Danni; Zhang, Renyin; Peng, Lizhi; Chen, Chuangfu

doi:10.1111/jam.14519

Cited by 13 publications

(14 citation statements)

References 14 publications

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“…The representative colored NPs widely used in this approach are colloidal AuNPs. In previous studies, AuNPs were generally applied to paper-based flow assay strips [ 74 , 75 , 76 , 111 ]. The concentrated NPs acted as colorimetric labels and generated color in the test zone.…”

Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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Bacterial contamination is a public health concern worldwide causing enormous social and economic losses. For early diagnosis and adequate management to prevent or treat pathogen-related illnesses, extensive effort has been put into the development of pathogenic bacterial detection systems. Colorimetric sensing systems have attracted increasing attention due to their simple and single-site operation, rapid signal readout with the naked eye, ability to operate without external instruments, portability, compact design, and low cost. In this article, recent trends and advances in colorimetric systems for the detection and monitoring of bacterial contamination are reviewed. This article focuses on pathogen detection strategies and technologies based on reaction factors that affect the color change for visual readout. Reactions used in each strategy are introduced by dividing them into the following five categories: external pH change-induced pH indicator reactions, intracellular enzyme-catalyzed chromogenic reactions, enzyme-like nanoparticle (NP)-catalyzed substrate reactions, NP aggregation-based reactions, and NP accumulation-based reactions. Some recently developed colorimetric systems are introduced, and their challenges and strategies to improve the sensing performance are discussed.

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Section: Current Colorimetric Sensing Systems For the Detection Of Ba...mentioning

confidence: 99%

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Kim

Yoo

2022

Biosensors

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“…Generally, expensive equipment prevents this method from being universally popularized. Therefore, it is necessary to design a convenient, rapid and economical diagnosis method [10]. Enzyme-linked immunosorbent assay (ELISA), the rose bengal test (RBT) and the standard tube agglutination test (SAT) can discover the existence of Brucella infection by detecting serum antibodies [11].…”

Section: Introductionmentioning

confidence: 99%

“…There are many methods available for the diagnosis of brucellosis in animals, such as serological, etiological, and molecular biological diagnostic procedures [10]. Although bacteriological diagnosis is the gold standard, it is not only time consuming and laborious but also can result in operator infection.…”

Section: Introductionmentioning

confidence: 99%

Specific Serodiagnosis of Animal Brucellosis Based on A Recombinant Multiepitope Protein Antigen

Xu¹,

Bai²,

Zhang³

et al. 2021

Preprint

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Background: Brucellosis is a zoonotic infectious disease that causes substantial public health problems and endangers the development of animal husbandry in endemic areas, causing huge losses of personal property. Early diagnosis of sick animals is a crucial step in reducing the incidence of brucellosis.Objective: In this study, we designed a recombinant multiepitope protein (rMEP) as a serum diagnostic antigen for brucellosis and evaluated its diagnostic value in cattle and goats.Methods: An indirect enzyme-linked immunosorbent assay (iELISA) was used to assess the new rMEP, and 159 goat and 153 bovine serum samples were measured, including brucellosis and nonbrucellosis samples. To better observe the effectiveness of rMEP, we performed receiver operating characteristic (ROC) curve analysis.Results: Evaluation of the 159 goat serum samples showed that the area under the ROC curve (AUC) was 0.9976, and compared with serum tube agglutination test (SAT) and the Rose Bengal plate agglutination test (RBPT), the positive and negative diagnostic accuracies of ELISA were 98.92% (92/93) and 96.97% (64/66), respectively. Evaluation of the 153 bovine serum samples showed that the AUC was 0.9974, and compared with those of SAT and RBPT, the positive and negative diagnostic accuracies of ELISA were 98.65% (73/74) and 96.20% (76/79), respectively.Conclusion: The results indicated that rMEP, as a protein antigen, can be used to diagnose brucellosis with high accuracy in both goats and bovines.

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“…There are many methods available for the diagnosis of brucellosis in animals, such as serological diagnosis, etiological diagnosis, molecular biology diagnosis, etc. [10]. Although the bacteriological diagnosis is the gold standard, it is not only time consuming and laborious, but also easily causes operator infection.…”

mentioning

confidence: 99%

“…Generally, the expensive equipment prevents this method from being universally popularised. Therefore, it is necessary to design a convenient, rapid and economical diagnosis method [10]. Enzyme-linked immunosorbent assay (ELISA), the rose bengal test (RBT) and standard tube agglutination test (SAT) can discover the existence of Brucella infection by detecting antibodies in the serum [11].…”

mentioning

confidence: 99%

Specific serodiagnosis of animal brucellosis based on a recombinant multiepitope protein antigen

Yin

Bai

et al. 2020

Preprint

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Background: Brucellosis is a vital zoonotic infectious disease that will cause important public health problems and endanger the development of animal husbandry in endemic areas, causing huge losses of personal property. Early diagnosis of sick animals is a crucial way to reduce the incidence of brucellosis.Results: In this study, we designed a recombinant multiepitope protein (rMEP) as a serum diagnostic antigen for brucellosis. An indirect enzyme-linked immunosorbent assay (ELISA) was used to assess the new rMEP, 159 goat and 153 bovine serum samples were measured, including brucellosis and non-brucellosis. To better observe the effectiveness of rMEP, we performed a receiver operating characteristic (ROC) analysis. The results of 159 goat serum evaluations showed that the area under the ROC curve(AUC) was 0.9976 and compared with SAT and RBPT, the positive and negative diagnostic accuracy of the ELISA was 98.92%(92/93) and 96.97%(64/66), respectively. 153 bovine serum samples evaluations showed the AUC was 0.9974 and compared with SAT and RBPT, the positive and negative diagnostic accuracy of the ELISA was 98.65%(73/74) and 96.20%(76/79), respectively.Conclusions: The results show that rMEP as a protein antigen can be used to diagnose brucellosis with highly accurate both in goat and bovine.

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A novel, rapid and simple method for detecting brucellosis based on rapid vertical flow technology

Cited by 13 publications

References 14 publications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Colorimetric Systems for the Detection of Bacterial Contamination: Strategy and Applications

Specific Serodiagnosis of Animal Brucellosis Based on A Recombinant Multiepitope Protein Antigen

Specific serodiagnosis of animal brucellosis based on a recombinant multiepitope protein antigen

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