A novel quorum quencher, Rhodococcus pyridinivorans XN-36, is a powerful agent for the biocontrol of soft rot disease in various host plants

Zhou, Zhe; Wu, Xiaozhen; Li, Jiayi; Zhang, Yiyin; Huang, Yaohua; Zhang, Wenping; Shi, Yu; Wang, Junxia; Chen, Shaohua

doi:10.1016/j.biocontrol.2022.104889

Cited by 15 publications

(3 citation statements)

References 62 publications

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“…Targeting QS pathways has gained popularity during the last several years because of its many applications within medicine and agriculture [ 42 , 43 , 44 ]. By interrupting the communication between bacteria, QS-regulated virulence factors can be suppressed [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

The Antibacterial Effect of Cannabigerol toward Streptococcus mutans Is Influenced by the Autoinducers 21-CSP and AI-2

et al. 2023

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Bacteria can communicate through an intercellular signaling system referred to as quorum sensing (QS). The QS system involves the production of autoinducers that interact with their respective receptors, leading to the induction of specific signal transduction pathways. The QS systems of the oral cariogenic Streptococcus mutans regulate the maturation of biofilms and affect its virulent properties. We have previously shown that the non-psychoactive compound cannabigerol (CBG) of the Cannabis sativa L. plant has anti-bacterial and anti-biofilm activities towards S. mutans. Here we were interested in investigating the effect of the two QS systems ComCDE and LuxS on the susceptibility of S. mutans to CBG and the anti-QS activities of CBG. This was assessed by using various comCDE and luxS mutant strains and complementation with the respective autoinducers, competence stimulating peptide (CSP) and (S)-4,5-dihydroxy-2,3-pentandione (DPD, pre-AI-2). We found that S. mutans comCDE knockout strains were more sensitive to the anti-bacterial actions of CBG compared to the WT strain. Exogenously added 21-CSP prevented the anti-bacterial actions caused by CBG on the ΔcomC, ΔcomE and ΔluxS mutants, while having no effect on the susceptibility of the WT and ΔcomCDE strains to CBG. Exogenously added DPD increased the susceptibility of WT and ΔluxS to CBG. Vice versa, CBG significantly reduced the 21-CSP-induced expression of comCDE genes and ComE-regulated genes and suppressed the expression of luxS with concomitant reduction in AI-2 production. DPD induced the expression of comCDE genes and ComE-regulated genes, and this induction was repressed by CBG. 21-CSP alone had no significant effect on luxS gene expression, while ΔcomCDE strains showed reduced AI-2 production. In conclusion, our study shows that the susceptibility of S. mutans to CBG is affected by the ComCDE and LuxS QS pathways, and CBG is a potential anti-QS compound for S. mutans. Additionally, we provide evidence for crosstalk between the ComCDE and LuxS QS systems.

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Section: Discussionmentioning

confidence: 99%

The Antibacterial Effect of Cannabigerol toward Streptococcus mutans Is Influenced by the Autoinducers 21-CSP and AI-2

et al. 2023

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“…To study the antagonistic effect of strain F25 with pathogenic bacteria, studies were carried out on LB solid plates ( Li et al., 2020 ; Zhou et al., 2022 ). A bacterial solution of Xcc was inoculated at 10% into the melted LB.…”

Section: Methodsmentioning

confidence: 99%

“…It also facilitates important biological functions such as extracellular product synthesis, bioluminescence, and the production of virulence factors ( Semighini et al., 2006 ; Deng et al., 2014 ; Bukvicki et al., 2016 ). Population sensing is a communication mechanism between bacteria, referring to the process by which bacteria adjust their population density and behavior by sensing the levels of various signaling molecules in their environment ( Ya'ar Bar et al., 2021 ; Liu et al., 2022 ; Zhou et al., 2022 ). Changes in the species composition and cell density of microbial communities can transmit information through complex signaling systems, allowing the bacteria to collectively change their behavior by exchanging information among themselves ( Wang et al., 2004 ; Whiteley et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

A novel bacterial strain Burkholderia sp. F25 capable of degrading diffusible signal factor signal shows strong biocontrol potential

Chen

Bhatt

et al. 2022

Front. Plant Sci.

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Vast quantities of synthetic pesticides have been widely applied in various fields to kill plant pathogens, resulting in increased pathogen resistance and decreased effectiveness of such chemicals. In addition, the increased presence of pesticide residues affects living organisms and the environment largely on a global scale. To mitigate the impact of crop diseases more sustainably on plant health and productivity, there is a need for more safe and more eco-friendly strategies as compared to chemical prevention. Quorum sensing (QS) is an intercellular communication mechanism in a bacterial population, through which bacteria adjust their population density and behavior upon sensing the levels of signaling molecules in the environment. As an alternative, quorum quenching (QQ) is a promising new strategy for disease control, which interferes with QS by blocking intercellular communication between pathogenic bacteria to suppress the expression of disease-causing genes. Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is associated with the diffusible signal factor (DSF). As detailed in this study, a new QQ strain F25, identified as Burkholderia sp., displayed a superior ability to completely degrade 2 mM of DSF within 72 h. The main intermediate product in the biodegradation of DSF was identified as n-decanoic acid, based on gas chromatography-mass spectrometry (GC-MS). A metabolic pathway for DSF by strain F25 is proposed, based on the chemical structure of DSF and its intermediates, demonstrating the possible degradation of DSF via oxidation-reduction. The application of strain F25 and its crude enzyme as biocontrol agents significantly attenuated black rot caused by Xcc, and inhibited tissue maceration in the host plant Raphanus sativus L., without affecting the host plant. This suggests that agents produced from strain F25 and its crude enzyme have promising applications in controlling infectious diseases caused by DSF-dependent bacterial pathogens. These findings are expected to provide a new therapeutic strategy for controlling QS-mediated plant diseases.

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Current trends in management of bacterial pathogens infecting plants

Sharma

Gupta

Devi

2023

Antonie van Leeuwenhoek

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A novel quorum quencher, Rhodococcus pyridinivorans XN-36, is a powerful agent for the biocontrol of soft rot disease in various host plants

Cited by 15 publications

References 62 publications

The Antibacterial Effect of Cannabigerol toward Streptococcus mutans Is Influenced by the Autoinducers 21-CSP and AI-2

The Antibacterial Effect of Cannabigerol toward Streptococcus mutans Is Influenced by the Autoinducers 21-CSP and AI-2

A novel bacterial strain Burkholderia sp. F25 capable of degrading diffusible signal factor signal shows strong biocontrol potential

Current trends in management of bacterial pathogens infecting plants

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