A Novel Pzg-NURF Complex Regulates Notch Target Gene Activity

Kugler, Sabrina J.; Nagel, Anja C.

doi:10.1091/mbc.e10-03-0212

Cited by 27 publications

(35 citation statements)

References 35 publications

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“…Specifically, in 1 mammalian cells, the histone acetyl transferases (HATs) PCAF (KAT2B) and GCN5 (KAT2A) have been shown biochemically to function in RBPJ [the mammalian homolog of Su(H)]-mediated transactivation by N ICD (Kurooka and Honjo, 2000). In flies, the RING-finger E3 ubiquitin ligase Bre1 and chromatin remodeling complex NURF have been shown to be involved in the epigenetic regulation of Notch target gene expression (Bray et al, 2005;Kugler and Nagel, 2010). Recently, an RNAi screen in cultured Drosophila cells revealed that a Notch pathway transcriptional reporter is sensitive to chromatin-modifying enzymes and remodelers (Mourikis et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development

Chen

et al. 2013

Development

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SUMMARYThe histone chaperone CAF-1 is known for its role in DNA replication-coupled histone deposition. However, loss of function causes lethality only in higher multicellular organisms such as mice and flies, but not in unicellular organisms such as yeasts, suggesting that CAF-1 has other important functions than histone deposition during animal development. Emerging evidence indicates that CAF-1 also has a role in higher order chromatin organization and heterochromatin-mediated gene expression; it remains unclear whether CAF-1 has a role in specific signaling cascades to promote gene expression during development. Here, we report that knockdown of one of the subunits of Drosophila CAF-1, dCAF-1-p105 (Caf1-105), results in phenotypes that resemble those of, and are augmented synergistically by, mutations of Notch positive regulatory pathway components. Depletion of dCAF-1-p105 leads to abrogation of cut expression and to downregulation of other Notch target genes in wing imaginal discs. dCAF-1-p105 is associated with Suppressor of Hairless [Su(H)] and regulates its binding to the enhancer region of E(spl)mβ. The association of dCAF-1-p105 with Su(H) on chromatin establishes an active local chromatin status for transcription by maintaining a high level of histone H4 acetylation. In response to induced Notch activation, dCAF-1 associates with the Notch intracellular domain to activate the expression of Notch target genes in cultured S2 cells, manifesting the role of dCAF-1 in Notch signaling. Together, our results reveal a novel epigenetic function of dCAF-1 in promoting Notch pathway activity that regulates normal Drosophila development.

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Section: Introductionmentioning

confidence: 99%

CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development

Chen

et al. 2013

Development

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“…At least three GAF-associated proteins with C2H2-type zinc finger motifs were confirmed to be GAF direct partners: CG2199, Pzg/Z4, and MEP-1 [163]. MEP-1 and Pzg/Z4 are known to be associated with chromatin remodeling activity [178,179]. Although GAF is a multifunctional factor implicated in different nuclear functions, these proteins can be implicated in the PcG/TrxG system as well.…”

Section: Much More Dna-binding Proteins Waits To Be Identifiedmentioning

confidence: 99%

Drosophila DNA-Binding Proteins in Polycomb Repression

2018

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Abstract:The formation of individual gene expression patterns in different cell types is required during differentiation and development of multicellular organisms. Polycomb group (PcG) proteins are key epigenetic regulators responsible for gene repression, and dysregulation of their activities leads to developmental abnormalities and diseases. PcG proteins were first identified in Drosophila, which still remains the most convenient system for studying PcG-dependent repression. In the Drosophila genome, these proteins bind to DNA regions called Polycomb response elements (PREs). A major role in the recruitment of PcG proteins to PREs is played by DNA-binding factors, several of which have been characterized in detail. However, current knowledge is insufficient for comprehensively describing the mechanism of this process. In this review, we summarize and discuss the available data on the role of DNA-binding proteins in PcG recruitment to chromatin.

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“…Many studies show interactions between components of the NURF complex, or the highly related FAC1 protein (Fetal Alz-50-reactive Clone 1), and sequence specific transcription factors (Table 1) [9,[53][54][55][56][57][58][59][60][61][62][63][64][65]. FAC1 has sequence identity to the N-terminus of the human NURF301 homolog BPTF, strongly suggesting the two are related [66,67].…”

Section: Nurf and Its Interaction With Chromatinmentioning

confidence: 99%

Nucleosome-Remodeling Factor

2013

Encyclopedia of Systems Biology

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An essential component of the chromatin remodeling machinery is NURF (Nucleosome Remodeling Factor), the founding member of the ISWI family of chromatin remodeling complexes. In vertebrates and invertebrates alike, NURF has many important functions in chromatin biology including regulating transcription, establishing boundary elements, and promoting higher order chromatin structure. Since NURF is essential to many aspects of chromatin biology, knowledge of its function is required to fully understand how the genome is regulated. This review will summarize what is currently known of its biological functions, conservation in the most prominent model organisms, biochemical functions as a nucleosome remodeling enzyme, and its possible relevance to human cancer.

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A Novel Pzg-NURF Complex Regulates Notch Target Gene Activity

Abstract: The Putzig (Pzg) protein is associated with the NURF nucleosome remodeling complex, thereby promoting Notch target gene expression. Our findings suggest a novel Pzg-NURF complex that is responsible for the epigenetic regulation of Notch target genes.

Cited by 27 publications

References 35 publications

CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development

CAF-1 promotes Notch signaling through epigenetic control of target gene expression during Drosophila development

Drosophila DNA-Binding Proteins in Polycomb Repression

Nucleosome-Remodeling Factor

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