A Novel Putrescine Utilization Pathway Involves γ-Glutamylated Intermediates of Escherichia coli K-12

Kurihara, Shin; Oda, Shinpei; Kato, Kenji; Kim, Hyeon Guk; Koyanagi, Takashi; Kumagai, Hidehiko; Suzuki, Hideyuki

doi:10.1074/jbc.m411114200

Cited by 142 publications

(168 citation statements)

References 30 publications

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“…6, supplemental Table 8). Slr1022 shows strong similarity to the proteins (YgjG and SpuC) involved in degradation of putrescine into aminobutyrate in E. coli and Pseudomonas (55,56). Further evidence that succinate is produced from arginine came from the strong up-regulation of succinate dehydrogenase and malate dehydrogenase (Fig.…”

Section: Alternate Pathway For Assimilation Of Nitrogen and Carbon Unmentioning

confidence: 99%

“…Putrescine is known to play a critical role in DNA, RNA, and protein synthesis, as well as in cell proliferation and differentiation. Furthermore, putrescine serves as a source for carbon and nitrogen in E. coli and Pseudomonas (55,56), where it is converted to succinate. Examination of proteins involved in putrescine degradation suggested that this pathway is also active in Synechocystis (Fig.…”

Section: Alternate Pathway For Assimilation Of Nitrogen and Carbon Unmentioning

confidence: 99%

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Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Wegener

Singh

Jacobs

et al. 2010

Molecular & Cellular Proteomics

111

133

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Section: Alternate Pathway For Assimilation Of Nitrogen and Carbon Unmentioning

confidence: 99%

Section: Alternate Pathway For Assimilation Of Nitrogen and Carbon Unmentioning

confidence: 99%

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Wegener

Singh

Jacobs

et al. 2010

Molecular & Cellular Proteomics

111

133

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“…Also, the putrescine degrading Puu pathway, which is responsible for catabolizing extracellular putrescine (Kurihara et al, 2005) in wild-type E. coli, was deleted; the puuPA genes that encode the dorminant putrescine importer PuuP and glutamate-putrescine ligase (PuuA; the first enzyme of the Puu pathway). However, an alternative putrescine degradation pathway involving YgjG and YdcW was not deleted because its activity is negligible (Kurihara et al, 2008).…”

Section: Construction Of a Base Strain For Putrescine Productionmentioning

confidence: 99%

“…Putrescine can be degraded through the Puu pathway with g-glutamylation (Kurihara et al, 2005) and the YdcW-YgjG pathway without g-glutamylation (Samsonova et al, 2003(Samsonova et al, , 2005. Moreover, putrescine is utilized as a precursor for the biosynthesis of other polyamines such as spermidine (Fig.…”

mentioning

confidence: 99%

Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine

Qian

Xia

Lee

2009

Biotech & Bioengineering

186

178

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A four carbon linear chain diamine, putrescine (1,4-diaminobutane), is an important platform chemical having a wide range of applications in chemical industry. Biotechnological production of putrescine from renewable feedstock is a promising alternative to the chemical synthesis that originates from non-renewable petroleum. Here we report development of a metabolically engineered strain of Escherichia coli that produces putrescine at high titer in glucose mineral salts medium. First, a base strain was constructed by inactivating the putrescine degradation and utilization pathways, and deleting the ornithine carbamoyltransferase chain I gene argI to make more precursors available for putrescine synthesis. Next, ornithine decarboxylase, which converts ornithine to putrescine, was amplified by a combination of plasmid-based and chromosome-based overexpression of the coding genes under the strong tac or trc promoter. Furthermore, the ornithine biosynthetic genes (argC-E) were overexpressed from the trc promoter, which replaced the native promoter in the genome, to increase the ornithine pool. Finally, strain performance was further improved by the deletion of the stress responsive RNA polymerase sigma factor RpoS, a well-known global transcription regulator that controls the expression of ca. 10% of the E. coli genes. The final engineered E. coli strain was able to produce 1.68 g L À1 of putrescine with a yield of 0.168 g g À1 glucose. Furthermore, high cell density cultivation allowed production of 24.2 g L À1 of putrescine with a productivity of 0.75 g L À1 h À1 . The strategy reported here should be useful for the bio-based production of putrescine from renewable resources, and also for the development of strains capable of producing other diamines, which are important as nitrogen-containing platform chemicals.

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“…PotE is responsible for both the excretion and uptake of putrescine (18). PuuP is the most recently discovered putrescine transporter and depends on proton motive force (19,20). In addition, two putative putrescine importers, YdcSTUV and YeeF, have been annotated by computer analysis.…”

mentioning

confidence: 99%

A Novel Putrescine Importer Required for Type 1 Pili-driven Surface Motility Induced by Extracellular Putrescine in Escherichia coli K-12

Kurihara

Suzuki

Oshida

et al. 2011

Journal of Biological Chemistry

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Recently, many studies have reported that polyamines play a role in bacterial cell-to-cell signaling processes. The present study describes a novel putrescine importer required for induction of type 1 pili-driven surface motility. The surface motility of the Escherichia coli ⌬speAB ⌬speC ⌬potABCD strain, which cannot produce putrescine and cannot import spermidine from the medium, was induced by extracellular putrescine. Introduction of the gene deletions for known polyamine importers (⌬potE, ⌬potFGHI, and ⌬puuP) or a putative polyamine importer (⌬ydcSTUV) into the ⌬speAB ⌬speC ⌬potABCD strain did not affect putrescine-induced surface motility. The deletion of yeeF, an annotated putative putrescine importer, in the ⌬speAB ⌬speC ⌬potABCD ⌬ydcSTUV strain abolished surface motility in putrescine-supplemented medium. Complementation of yeeF by a plasmid vector restored surface motility. The surface motility observed in the present study was abolished by the deletion of fimA, suggesting that the surface motility is type 1 pili-driven. A transport assay using the yeeF ؉ or ⌬yeeF strains revealed that YeeF is a novel putrescine importer. The K m of YeeF (155 M) is 40 to 300 times higher than that of other importers reported previously. On the other hand, the V max of YeeF (9.3 nmol/min/mg) is comparable to that of PotABCD, PotFGHI, and PuuP. The low affinity of YeeF for putrescine may allow E. coli to sense the cell density depending on the concentration of extracellular putrescine.

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A Novel Putrescine Utilization Pathway Involves γ-Glutamylated Intermediates of Escherichia coli K-12

Cited by 142 publications

References 30 publications

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Global Proteomics Reveal an Atypical Strategy for Carbon/Nitrogen Assimilation by a Cyanobacterium Under Diverse Environmental Perturbations

Metabolic engineering of Escherichia coli for the production of putrescine: A four carbon diamine

A Novel Putrescine Importer Required for Type 1 Pili-driven Surface Motility Induced by Extracellular Putrescine in Escherichia coli K-12

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