A novel pulsed drug-delivery system: polyelectrolyte layer-by-layer coating of chitosan&amp;ndash;alginate microgels

Zhou, Guichen; Lü, Ying; Zhang, He; Chen, Yan; Yuan, Y. Connie; Gao, Jing; Sun, Duxin; Zhang, Guoqing; Zou, Hao; Zhong, Yanqiang

doi:10.2147/ijn.s38144

Cited by 11 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability of translocation through the membrane of GPMVs was tested with two different substances, Calcein AM and fluorescently labeled dextran (MW 10.000), which may be also used to study drug delivery vehicles [ 35 ]. Calcein is also commonly used as an indicator of lipid vesicles leakage [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

GPMVs in variable physiological conditions: could they be used for therapy delivery?

et al. 2018

View full text Add to dashboard Cite

BackgroundCell based carriers are increasingly recognized as a good system for cargo delivery to cells. One of the reasons is their biocompatibility and low toxicity compared to artificial systems. Giant plasma membrane vesicles (GPMV) derive from the cell plasma membrane. Thus they offer the closest approximation to it, which makes them good candidates for potential drug delivery systems. To evaluate the applicability of GPMVs as carriers, we analyzed their basic biophysical properties to test their robustness in the face of changeable physiological conditions, as well as their ability to translocate across the membrane into cells.ResultsGPMVs formed from human umbilical vein endothelial cells (HUVEC) sustain a drastic osmotic challenge (50–500 mOsmoL/kg) unlike giant unilamelar vesicles (GUVs). In hyper-osmotic solutions the average volume decreases and membrane invaginations form, while in the hypo-osmolar buffer the volume of GPMVs increases and these changes were not reversible. The membranes of flaccid GPMVs started to wrinkle unevenly giving rise to buds after exposure to lipopolysaccharide (LPS). The shape changes in GUVs are reversible in contrast to GPMVs after LPS removal. GPMVs exposed to fluorescent LPS exhibited a signal that remained visible in some GPMVs even after LPS removal, which was never the case with GUVs. Calcein penetrated both into GUVs and GPMVs, however after the removal from the bulk solution some of the GPMVs still exhibited very bright signal, while in GUVs only a weak fluorescent signal was detected. We could also see that practically all GPMVs incorporated dextran initially, but after the dextran solution was changed with the initial non-fluorescent solution it remained only in 20% of them. The majority of HUVEC cells displayed a fluorescent signal after the incubation with GPMVs that contained fluorescently labeled dextran.ConclusionOur findings indicate that GPMVs behave quite differently from artificially made giant phospholipid vesicles and the changes induced by the different treatments we subjected them to are not reversible. We also demonstrate that different substances can be both loaded into them and delivered into cells, so GPMVs may be of potential use as cargo/therapy delivery systems.

show abstract

Section: Discussionmentioning

confidence: 99%

GPMVs in variable physiological conditions: could they be used for therapy delivery?

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In addition, the use of a noncovalent route for multilayer formation provides a lowcost simple fabrication method [48]. Due to these promising advantages of the layer-by-layer method, it has been widely used to fabricate nanoparticles for drug delivery [44][45][46][47]49]. Apart from engineering the size and surface properties of nanocarriers, incorporation of magnetic nanoparticles (MNPs) enables the targeted delivery of the drug carriers to tumour sites with the help of an external magnetic field [50].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Alginate / Chitosan Nanoparticles for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Song

Gregory

et al. 2018

Preprint

View full text Add to dashboard Cite

Curcumin is a promising anti-cancer drug but its applications in cancer therapy are limited due to its poor solubility, short half-life and low bioavailability. In this study, curcumin loaded magnetic alginate / chitosan nanoparticles were fabricated to improve the bioavailability, uptake efficiency and cytotoxicity of curcumin to MDA-MB-231 breast cancer cells. Alginate and chitosan were deposited on Fe3O4 magnetic nanoparticles based on their electrostatic properties. The sizes of the nanoparticles (120-200 nm) were within the optimum range for drug delivery. Sustained curcumin release was obtained use the nanoparticles with the ability to control the curcumin release rate by altering the number of chitosan and alginate layers. Confocal fluorescence microscopy results showed that targeted delivery of curcumin with the aid of magnetic field were achieved. The FACS assay indicated that MDA-MB-231 cells treated with curcumin loaded nanoparticles had a 3-6 folds uptake efficiency to those treated with free curcumin. MTT assay indicated that the curcumin loaded nanoparticles exhibited significantly higher cytotoxicity toward MDA-MB-231 cells than toward HDF cells. The sustained release profiles, enhanced uptake efficiency and cytotoxicity to cancer cells as well as the targeting potential make MACPs a promising candidate for cancer therapy.

show abstract

“…Given the current knowledge of chitosan-coated alginate microgel formation [49,[60][61][62] and the more hydrophobic nature of chitosan compared to other polysaccharides, chitosan-coated alginate microparticles could be used to form and stabilize emulsions. Emulsion stabilization could then be combined with their encapsulation properties, which could be useful for protection or controlled release of useful active substances [63].…”

Section: Introductionmentioning

confidence: 99%

Alginate-Chitosan Microgel Particles, Water–Oil Interfacial Layers, and Emulsion Stabilization

Charisis

Kalogianni

2023

Colloids and Interfaces

View full text Add to dashboard Cite

In this work, alginate-chitosan microgel particles were formed at different pH levels with the aim of using them as viscoelastic interfacial layers, which confer emulsion stability to food systems. The particles’ size and structural characteristics were determined using laser diffraction, confocal laser microscopy (CLSM), and time-domain nuclear magnetic resonance (TD-NMR). The pH affected the microgel characteristics, with larger particles formed at lower pH levels. T2 relaxation measurements with TD-NMR did not reveal differences in the mobility within the particles for the different pH levels, which could have been related to the more or less swollen structure. The rate of adsorption of the particles at the sunflower oil–water interface differed between particles formed at different pH levels, but the equilibrium interfacial tension of all systems was similar. Higher interfacial dilatational viscoelasticity was obtained for the systems at lower pH (3, 4, 5), with G’ reaching 13.6 mN/m (0.1 Hz) at pH 3. The interfacial rheological regime transitioned from a linear elastic regime at lower pH to a linear but more viscoelastic one at higher pH. The thicker, highly elastic interfacial layer at low pH, in combination with the higher charges expected at lower pH, was related to its performance during emulsification and the performance of the emulsion during storage. As revealed by laser diffraction and CLSM, the droplet sizes of emulsions formed at pH 6 and 7 were significantly larger and increased in size during 1 week of storage. CLSM examination of the emulsions revealed bridging flocculation with the higher pH. Nevertheless, all emulsions formed with microgel systems presented macroscopic volumetric stability for periods exceeding 1 week at 25 °C. A potential application of the present systems could be in the formation of stable, low-fat dressings without the addition of any emulsifier, allowing, at the same time, the release of the bioactive compounds for which such particles are known.

show abstract

A novel pulsed drug-delivery system: polyelectrolyte layer-by-layer coating of chitosan–alginate microgels

Cited by 11 publications

References 31 publications

GPMVs in variable physiological conditions: could they be used for therapy delivery?

GPMVs in variable physiological conditions: could they be used for therapy delivery?

Magnetic Alginate / Chitosan Nanoparticles for Targeted Delivery of Curcumin into Human Breast Cancer Cells

Alginate-Chitosan Microgel Particles, Water–Oil Interfacial Layers, and Emulsion Stabilization

Contact Info

Product

Resources

About