A novel process for size controlled biosynthesis of gold nanoparticles using bromelain

Khan, Salman; Rizvi, Syed Mohd Danish; Avaish, Mohd; Arshad, Mohd Khairuddin; Bagga, Pramdeep; Khan, Mohd Sajid

doi:10.1016/j.matlet.2015.06.118

Cited by 44 publications

(40 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So, instead of using chemicals, we used a natural enzyme bromelain to produce biocompatible and non‐toxic gold nanoparticles. Gold nanoparticles were prepared according to the one‐pot synthesis method where bromelain was used for both reduction and capping as mentioned in our previous work [Khan et al, ]. Further, cefotaxime was conjugated to gold nanoparticles with the help of activator 1‐Ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug-Resistant CTX-M-Producing Bacterial Pathogens

et al. 2017

View full text Add to dashboard Cite

Multidrug-resistance due to "β lactamases having the expanded spectrum" (ESBLs) in members of Enterobacteriaceae is a matter of continued clinical concern. CTX-M is among the most common ESBLs in Enterobacteriaceae family. In the present study, a nanoformulation of cefotaxime was prepared using gold nanoparticles to combat drug-resistance in ESBL producing strains. Here, two CTX-M-15 positive cefotaxime resistant bacterial strains (i.e., one Escherichia coli and one Klebsiella pneumoniae strain) were used for testing the efficacy of "cefotaxime loaded gold-nanoparticles." Bromelain was used for both reduction and capping in the process of synthesis of gold-nanoparticles. Thereafter, cefotaxime was conjugated onto it with the help of activator 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide. For characterization of both unconjugated and cefotaxime conjugated gold nanoparticles; UV-Visible spectroscopy, Scanning, and Transmission type Electron Microscopy methods accompanied with Dynamic Light Scattering were used. We used agar diffusion method plus microbroth-dilution method for the estimation of the antibacterial-activity and determination of minimum inhibitory concentration or MIC values, respectively. MIC values of cefotaxime loaded gold nanoparticles against E. coli and K. pneumoniae were obtained as 1.009 and 2.018 mg/L, respectively. These bacterial strains were completely resistant to cefotaxime alone. These results reinforce the utility of conjugating an old unresponsive antibiotic with gold nanoparticles to restore its efficacy against otherwise resistant bacterial pathogens. J. Cell. Biochem. 118: 2802-2808, 2017. © 2017 Wiley Periodicals, Inc.

show abstract

Section: Resultsmentioning

confidence: 99%

“…Method of Khan et al [] was used for gold‐nanoparticle synthesis. First, 3 µl of 1.0 mM solution (Chloroauric acid) was mixed with 3 ml of Phosphate buffer (50 mM) containing 0.33 mg/ml bromelain.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug-Resistant CTX-M-Producing Bacterial Pathogens

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Consequently, there arose a demand for green nanotechnology that comprises clean, safe, and environmentally friendly systems of nanoparticles fabrication. 2 The biological technique, which is a key component of the green nanotechnology has expanded in the last decade to biosynthesize metallic nanoparticles owing to abundance of a wide-range of biological resources such as bacteria, [3][4][5][6][7] fungi, 8 plants' extracts, [9][10][11][12][13][14] enzymes, [15][16][17][18][19][20][21] as well as metabolites of arthropods. [22][23][24][25][26][27][28][29] There are outstanding reviews on the use of pigments, agrowastes, enzymes, arthropods, and their metabolites for the ecofriendly and benign synthesis of nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Silver‐gold alloy nanoparticles biofabricated by fungal xylanases exhibited potent biomedical and catalytic activities

Elegbede

Lateef

Azeez

et al. 2019

Biotechnology Progress

View full text Add to dashboard Cite

The search for biocompatible nanoparticles with vast applicability has impacted on exploration of various biomaterials for the synthesis of mono and bimetallic nanoparticles. Xylanase is widely regarded as an industrially important enzyme but its potentials in nanotechnological applications are yet to be fully explored. The current study investigates the exploit of xylanases of Aspergillus niger L3 (NE) and Trichoderma longibrachiatum L2 (TE) produced through valorization of corn‐cob, to synthesize silver‐gold alloy nanoparticles (Ag‐AuNPs). Characterization of the Ag‐AuNPs involved UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), and field emission scanning electron microscopy and transmission electron microscopy, while their prospective use as antimicrobial, antioxidant, catalytic, anticoagulant, and thrombolytic agents were studied. The biosynthesized Ag‐AuNPs were ruby red and light purple with surface plasmon resonance at 520 and 534 nm for NEAg‐AuNPs and TEAg‐AuNPs, respectively; while FTIR showed that protein molecules capped and stabilized the nanoparticles. The Ag‐AuNPs were anisotropic with spherical, oval, and irregular shapes having sizes ranging from 6.98 to 52.51 nm. The nanoparticles appreciably inhibited the growth of tested clinical bacteria (23.40–90.70%) and fungi (70.10–89.05%), and also scavenged 2,2‐diphenyl‐1‐picrylhydrazyl (48.51–53.79%) and hydrogen peroxide (80.5–95.50%). Furthermore, the Ag‐AuNPs degraded malachite green (91.39%) and methylene blue (47.10%). Moreover, the Ag‐AuNPs displayed outstanding anticoagulant and thrombolytic activities using human blood. This study further emphasizes the significance of xylanases in nanobiotechnology as it has established the potential of xylanases to synthesize Ag‐AuNPs, which is being reported for the first time.

show abstract

“…To ensure the formation of nanoparticles, the samples were removed from the reaction mixture at regular intervals and analyzed by UV-visible spectroscopy. On completion of the reaction, Au-NPs were collected by centrifugation at 30000 g for 30 min, washed twice with MilliQ water and the excess bromelain was removed by treating with 50 % ethanol (v/v) (Khan et al, 2015[ 15 ]).…”

Section: Methodsmentioning

confidence: 99%

Bromelain capped gold nanoparticles as the novel drug delivery carriers to aggrandize effect of the antibiotic levofloxacin

Bagga

Ansari

Siddiqui

et al. 2016

EXCLI Journal; 15:Doc772; ISSN 1611-2156

Self Cite

View full text Add to dashboard Cite

A novel process for size controlled biosynthesis of gold nanoparticles using bromelain

Cited by 44 publications

References 13 publications

Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug-Resistant CTX-M-Producing Bacterial Pathogens

Synthesis and Characterization of Cefotaxime Conjugated Gold Nanoparticles and Their Use to Target Drug-Resistant CTX-M-Producing Bacterial Pathogens

Silver‐gold alloy nanoparticles biofabricated by fungal xylanases exhibited potent biomedical and catalytic activities

Bromelain capped gold nanoparticles as the novel drug delivery carriers to aggrandize effect of the antibiotic levofloxacin

Contact Info

Product

Resources

About