“…The microbiological quality of HMMC at the beginning of storage varied greatly in different studies, with initial counts for Enterobacteriaceae and Pseudomonas ranging from 10 1 to 10 2 and from 10 0 e10 4 , respectively (Conte et al, 2007;Conte et al, 2013;Del Nobile et al, 2009;Faccia et al, 2013;Gammariello et al, 2008;Lucera et al, 2014;Sinigaglia et al, 2008;). Deterioration of sensory quality has been associated with counts of Pseudomonas exceeding 10 6 cfu/g while Enterobacteriaceae seem to be less important, and has been found to occur between 3 and 12 days of refrigerated storage (4e8 C) when no additional preservation method was used (Conte et al, 2013;Lucera et al, 2014) In some cases, samples have been found to be still acceptable with Pseudomonas counts between 7 and 7.2 log(cfu/g) Faccia et al, 2013). Shelf life assessed by evaluation of microbial counts is usually shorter (2e8 days at temperatures ranging from 4 to 15 C) and the end of shelf life is usually determined by either coliforms/Enterobacteriaceae or Pseudomonas counts exceeding an arbitrary limit (usually 10 5 and 5 Â 10 5 e1 Â 10 6 cfu/ ml, respectively; (Conte et al, 2007;Sinigaglia et al, 2008).…”