A novel polysaccharide from the roots of Millettia Speciosa Champ: preparation, structural characterization and immunomodulatory activity

Huang, Zhi; Zeng, Ying‐Jie; Chen, Xi; Luo, Siyuan; Pu, Lei; Li, Fangzhou; Zong, Min‐Hua; Lou, Wen-Yong

doi:10.1016/j.ijbiomac.2019.12.166

Cited by 59 publications

(35 citation statements)

References 54 publications

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“…It has a long history of medicinal use and is commonly used to treat chronic bronchitis, hepatitis, rheumatoid arthritis, and diabetes (24). Recent studies have shown that M. speciosa has immunomodulatory, anti-fatigue, antioxidant, hypoglycemic, and hypolipidemic effects (24)(25)(26). Polysaccharide is the main bioactive component of M. speciosa roots (MSCP), and in vitro immunomodulatory assays have demonstrated that MSCP2 could improve phagocytosis and stimulate cytokine and NO production (26).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have shown that M. speciosa has immunomodulatory, anti-fatigue, antioxidant, hypoglycemic, and hypolipidemic effects ( 24 – 26 ). Polysaccharide is the main bioactive component of M. speciosa roots (MSCP), and in vitro immunomodulatory assays have demonstrated that MSCP2 could improve phagocytosis and stimulate cytokine and NO production ( 26 ). However, we found that few studies on the in vivo immune activity of MSCP have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Chen

Sun

et al. 2021

Front. Immunol.

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Cyclophosphamide (CTX), a common anticancer drug, can cause a variety of side effects such as immunosuppression and intestinal mucosal injury. Polysaccharides are the major bioactive components of the roots of Millettia Speciosa Champ and have gained attention for their immunomodulatory activity. This study was designed to evaluate the immunomodulatory effect of Millettia Speciosa Champ polysaccharide (MSCP) on CTX-induced mice and the possible mechanism. The results showed that MSCP attenuated the CTX-induced decrease in body weight and immune organ indices in mice and promoted the secretion of immune-related cytokines (IL-2, IL-4, IL-10, TNF-α, and IgG). Meanwhile, MSCP restored intestinal morphology, increased the ratio of villus height/crypt depth (V/C), and improved the number of goblet cells and mucins expression. At the mRNA level, MSCP activated the TLRs/MyD88/NF-κB p65 pathway and enhanced the expression of genes related to intestinal mucosal integrity (Occludin1, Claudin1, and MUC-2). In addition, MSCP as a prebiotic improved microbial community diversity, regulated the relative abundance of dominant microbiota from the phylum level to the genus level, restored CTX-induced gut microbial dysbiosis, and promoted short-chain fatty acid production in mice. Based on the present findings, MSCP may modulate the immune response depending on enhancing intestinal health, suggesting that MSCP holds promise as a promising immunostimulant in functional foods and drugs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Chen

Sun

et al. 2021

Front. Immunol.

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“…The intensity of the absorption peak was proportional to the contents of sulphate group (Xiao et al, 2019). The absorption bands at 1046 and 820 cm −1 were attributed to pyranose ring structure and α-glycosidic bonds, respectively (Huang et al, 2020). In general, the FT-IR spectra of SPP-30 and SPP-90 were very similar, while the difference of SPP-60 was mainly reflected in the absorption peak of β-glycosidic bond near 900 cm −1 .…”

Section: Ft-ir Spectrummentioning

confidence: 90%

Physicochemical properties and bioactivity of polysaccharides from Sargassum pallidum by fractional ethanol precipitation

Zhang

Yuan

et al. 2021

Int J of Food Sci Tech

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Three polysaccharides (SPP-30, SPP-60 and SPP-90) were fractionated from Sargassum pallidum by 30%, 60% and 90% (v/v) ethanol, successively. Their physicochemical properties, antioxidant and antibacterial activities of SPP-30, SPP-60 and SPP-90 were comparatively investigated. The results showed that three polysaccharide fractions contained different neutral sugar, protein, uronic acid and sulphate contents. SPP-30 had the highest molecular weight (3.77-206.73 kDa), followed by SPP-60 (5.55-189.44 kDa) and SPP-90 (4.92 kDa). SPP-30, SPP-60 and SPP-90 were composed of fucose, arabinose, galactose, glucose, xylose and mannose but with different molar ratios. Scanning electron microscopy analysis suggested that they had different surface morphology and sizes of fragmentation. In vitro antioxidant assays suggested that SPP-30 and SPP-90 exhibited stronger scavenging activities against DPPH, ABTS and hydroxyl radicals than SPP-60. Moreover, SPP-30 and SPP-60 exhibited antibacterial activities against Staphylococcus aureus, while SPP-90 exhibited moderate antibacterial activity against both Staphylococcus aureus and Escherichia coli.

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“…Furthermore, the absorption at 1149, 1078, and 1019 cm −1 indicated a pyranose form of sugars, and the absorptions at 1078, and 1019 cm −1 indicated furanose form of sugars (Fan et al, 2020). In addition, the characteristic absorption band at 929 cm −1 revealed β-type glycosidic linkages, while 846 cm −1 was assigned to α-type glycosidic linkages (Dong et al, 2020;Huang et al, 2020).…”

Section: Analysis Of Ft-ir Spectra Of Apmentioning

confidence: 95%

“…The Fourier transform infrared (FT-IR) spectrum of AP was obtained by using a FT-IR spectrometer (PerkinElmer Frontier™, USA) at the range of 4000-400 cm −1 (Huang et al, 2020).…”

Section: Analysis Of Ft-ir Spectrometrymentioning

confidence: 99%

A novel polysaccharide from plant fermentation extracts and its immunomodulatory activity in macrophage RAW264.7 cells

Yan

Liu

Yan

et al. 2021

Food and Agricultural Immunology

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Plant fermentation extracts (PFEs) produced from fermenting various fruits and vegetables by microorganisms have traditionally been consumed as healthy beverages in Asia. Herein, the immunomodulatory activities of a novel polysaccharide (AP) isolated from PFE were examined in RAW264.7 cells. The results showed that AP was composed of Rha, Xyl, Man and Glu in a molar ratio of 9.7:27.6:8.81:53.85 and the average molecular weight was 4.81 × 10 5 Da. Our data showed that AP could significantly enhance the phagocytic activity of RAW264.7 cells and their ability to release NO, TNF-α, IL-6 and IL-10. Furthermore, AP could significantly up-regulate the mRNA expression of iNOS, cytokines (TNF-α, IL-6, IL-10) and pattern recognition receptors (TLR2, MR, GR, CR3 and SR). Western blotting demonstrated that the regulation of NO and TNF-α were mediated through the NF-κB and MAPK signalling pathways. These findings helped to elucidate the immunomodulatory properties of the polysaccharide AP and PFE.

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A novel polysaccharide from the roots of Millettia Speciosa Champ: preparation, structural characterization and immunomodulatory activity

Cited by 59 publications

References 54 publications

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Polysaccharides From the Roots of Millettia Speciosa Champ Modulate Gut Health and Ameliorate Cyclophosphamide-Induced Intestinal Injury and Immunosuppression

Physicochemical properties and bioactivity of polysaccharides from Sargassum pallidum by fractional ethanol precipitation

A novel polysaccharide from plant fermentation extracts and its immunomodulatory activity in macrophage RAW264.7 cells

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