A novel polymorphisms in intron 12 of the bovine calpastatin gene

Juszczuk-Kubiak, Edyta; Wyszyńska-Koko, Joanna; Wicińska, K.; Rosochacki, S. J.

doi:10.1007/s11033-006-9048-0

Cited by 17 publications

(6 citation statements)

References 25 publications

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“…The size of skeletal muscle fibers, the muscle protein synthesis rate, and the muscle protein degradation rate are among the characteristics that are the most affected by calpastatin gene polymorphism in pigs [20]. With the calpain system playing an important role in muscle fiber growth [20,21], and with the calpain system activity decreasing with the increase in CAST gene activity [20], the calpain activity may play an imperative role that is necessary for cell proliferation, myoblast fusion, fibers growth, and fiber numbers [22]. The calpain system influence can also be observed in fiber numbers, by modulating myoblast fusion and changing the myoblast proliferation rate [23].…”

Section: Resultsmentioning

confidence: 99%

Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Jawasreh

Al-Amareen

Aad

2019

Animals

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Simple SummaryThe Awassi sheep is a very important breed, and using molecular selection to enhance meat production is important. Following genotyping of Awassi sheep housed at two different research stations in Jordan, three genotypes were identified for the calpastatin Hha1 gene. Following a fattening trial, the polymorphic calpastatin gene affected final (marketing) body weight and longissimus muscle width. Hha1 restriction sites found in Calpastatin gene can be used for molecular marker-assisted selection in Awassi for meat purposes.AbstractAdvances in molecular genetics have allowed the identification of genes that can enhance livestock production. The aim of this study was to investigate possible relationships between the calpastatin (CAST) Hha1 gene polymorphisms and growth performance, carcass characteristics, and meat quality in Awassi sheep. A total of 87 blood samples were collected from two-week-old Awassi ram lambs. The amplification of the CAST Hha1 gene yielded a fragment of 622 bp. Three CAST genotypes were found in Awassi sheep: MM for two fragments (385 bp and 281 bp), MN for three fragments (622 bp, 385 bp, and 281 bp), and NN for only one fragment (622 bp). The M and N allele frequencies of the CAST Hha1 genotypes were 0.765 and 0.235, respectively, while the genotypic frequencies of MM, MN, and NN were 0.586, 0.356, and 0.057, respectively. Based on CAST Hha1 gene polymorphisms, three groups of lambs (MM: n = 8; MN: n = 6; and NN: n = 3 genotypes) were subjected to a fattening period of 70 days to investigate growth performance and meat characteristics. Only the final body weight and longissimus muscle width were significantly different between the three genotypes, while no significant differences were detected in any other carcass characteristics and meat quality parameters. In this study, new variants were observed in CAST using the Hha1 restriction site, potentially assisting in Awassi sheep breeding and selection programs to improve final body weight and longissimus muscle width.

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Section: Resultsmentioning

confidence: 99%

Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Jawasreh

Al-Amareen

Aad

2019

Animals

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“…Similarly, postmortem proteolysis of muscle occurred in both control and calpain3-knockout mice ( Geesink et al, 2005 ). Calpastatin, the specific inhibitor of calcium-dependent proteases, μ-calpain and m-calpain ( Juszczuk-Kubiak et al, 2008 ) was shown to be involved in in vivo cell viability and cell proliferation ( Van Ba et al, 2015 ). Further, over-expression of calpastatin significantly inhibited calpain activity in muscle, heart, and neuronal tissue ( Li et al, 2009 ; Liang et al, 2010 ).…”

Section: Introductionmentioning

confidence: 99%

Association of μ-Calpain and Calpastatin Polymorphisms with Meat Tenderness in a Brahman–Angus Population

et al. 2018

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Autogenous proteolytic enzymes of the calpain family are implicated in myofibrillar protein degradation. As a result, the μ-calpain gene and its specific inhibitor, calpastatin, have been repeatedly investigated for their association with meat quality traits in cattle; however, no functional mutation has been identified for these two genes. The objectives of this study were: (1) to assess breed composition effect on tenderness; (2) to perform a linkage disequilibrium (LD) analysis in μ-calpain and calpastatin genes as well as an association analyses with tenderness; and (3) to analyze putative functional SNPs inside the significant LD block for an effect on tenderness. Tenderness measurements and genotypes for 16 SNPs in μ-calpain gene and 28 SNPs in calpastatin gene from 673 steers were analyzed. A bioinformatic analysis identified “putative functional SNPs” inside the associated LD block – polymorphisms able to produce a physical and/or chemical change in the DNA, mRNA, or translated protein in silico. Breed composition had a significant (P < 0.0001) effect on tenderness where animals with more than 80% Angus composition had the most tender meat. One 11-kb LD-block and three LD-blocks of 37, 17, and 14 kb in length were identified in the μ-calpain and calpastatin genes, respectively. Out of these, the LD-block 3 in calpastatin, tagged by SNPs located at 7-98566391 and 7-98581038, had a significant effect on tenderness with the TG-CG diplotype being approximately 1 kg more tender than the toughest diplotype, TG-CG. A total of 768 SNPs in the LD-block 3 of calpastatin were included in the bioinformatic analysis, and 28 markers were selected as putative functional SNPs inside the LD-block 3 of calpastatin; however, none of them were polymorphic in this population. Out of 15 initial polymorphisms segregating inside the LD-block 3 of calpastatin in this population, markers ARSUSMARC116, Cast5, rs730723459, and rs210861835 were found to be significantly associated with tenderness.

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“…A total of three highly significant polymorphisms were identified inside or close to μ -calpain for WBSF (BTA29: 44,117,703 bp), tenderness (BTA29: 44,067,968 bp) and connective tissue (BTA29: 44,067,968 bp). The μ-calpain and its inhibitor Calpastatin (CAST) are responsible for postmortem muscle proteolysis, in vivo cell viability, and cell proliferation ( Geesink, 2005 ; Geesink et al, 2006 ; Juszczuk-Kubiak et al, 2008 ; Van Ba et al, 2015 ). Leal-Gutiérrez et al (2019) did not identify an association between μ -calpain and meat quality in a Brahman-Angus population; however, Wright et al (2018) reported an association between Desmin (DES) and Titin (TTN) degradation and μ-calpain autolysis using a subset of individuals from the same population.…”

Section: Discussionmentioning

confidence: 99%

Whole Genome Sequence Data Provides Novel Insights Into the Genetic Architecture of Meat Quality Traits in Beef

et al. 2020

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Tenderness is a major quality attribute for fresh beef steaks in the United States, and meat quality traits in general are suitable candidates for genomic research. The objectives of the present analysis were to (1) perform genome-wide association (GWA) analysis for marbling, Warner-Bratzler shear force (WBSF), tenderness, and connective tissue using whole-genome data in an Angus population, (2) identify enriched pathways in each GWA analysis; (3) construct a protein-protein interaction network using the associated genes and (4) perform a µ-calpain proteolysis assessment for associated structural proteins. An Angus-sired population of 2,285 individuals was assessed. Animals were transported to a commercial packing plant and harvested at an average age of 457 ± 46 days. After 48 h postmortem, marbling was recorded by graders' visual appraisal. Two 2.54-cm steaks were sampled from each muscle for recording of WBSF, and tenderness, and connective tissue by a sensory panel. The relevance of additive effects on marbling, WBSF, tenderness, and connective tissue was evaluated on a genome-wide scale using a two-step mixed model-based approach in single-trait analysis. A tissue-restricted gene enrichment was performed for each GWA where all polymorphisms with an association p-value lower than 1 × 10 −3 were included. The genes identified as associated were included in a protein-protein interaction network and a candidate structural protein assessment of proteolysis analyses. A total of 1,867, 3,181, 3,926, and 3,678 polymorphisms were significantly associated with marbling, WBSF, tenderness, and connective tissue, respectively. The associate region on BTA29 (36,432,655-44,313,046 bp) harbors 13 highly significant markers for meat quality traits. Enrichment for the GO term GO:0005634 (Nucleus), which includes transcription factors, was evident. The final protein-protein network included 431 interations between 349 genes. The 42 most important genes based on significance that encode structural proteins were included in a proteolysis analysis, and 81% of these proteins were

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A novel polymorphisms in intron 12 of the bovine calpastatin gene

Cited by 17 publications

References 25 publications

Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Relationships between Hha1 Calpastatin Gene Polymorphism, Growth Performance, and Meat Characteristics of Awassi Sheep

Association of μ-Calpain and Calpastatin Polymorphisms with Meat Tenderness in a Brahman–Angus Population

Whole Genome Sequence Data Provides Novel Insights Into the Genetic Architecture of Meat Quality Traits in Beef

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