A novel pilot animal model for the surgical prevention of lymphedema: the power of optical imaging

Tran, Bich; Angelo, Joseph; Lee, Jeong Heon; Ruan, Qing; Laurence, Rita G.; Choi, Hak; Lee, Bernard T.; Singhal, Dhruv

doi:10.1016/j.jss.2017.08.029

Cited by 11 publications

(10 citation statements)

References 31 publications

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“…These studies also include genetic models and the assessment of response to pharmacotherapies. A pilot animal model to evaluate LYMPHA, or immediate lymphatic reconstruction, was recently developed and real‐time lymphatic clearance was quantified using novel fluorophores (Figure ) . This study reported a 68% reduction in lymphatic clearance from a swine hind limb after lymphadenectomy.…”

Section: Development Of An Animal Modelmentioning

confidence: 98%

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Immediate lymphatic reconstruction

Johnson

Singhal

2018

Journal of Surgical Oncology

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Although surgical and medical treatment options are available for the treatment of chronic lymphedema, there is no cure. Recent advances in microsurgery have provided an opportunity to perform immediate lymphatic reconstruction after lymphadenectomy for disease prevention. In this review, we provide the historical background leading to a paradigm shift in performing this procedure. We will also discuss the current evidence for immediate lymphatic reconstruction, potential oncologic procedures amenable to this approach, and detail ongoing challenges.

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Section: Development Of An Animal Modelmentioning

confidence: 98%

“…Pending copyright clearance. From figure of Tran et al [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: Development Of An Animal Modelmentioning

confidence: 99%

Immediate lymphatic reconstruction

Johnson

Singhal

2018

Journal of Surgical Oncology

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“…Finally, a pre‐clinical pig model has been established, demonstrating that LYMPHA alleviates the reduction in lymphatic clearance after groin lymphadenectomy (reduction in clearance: 68% vs 21%) . Taken together, these findings provide physiologic and clinical evidence that LYMPHA reduces postoperative swelling in the acute setting and after an extended period of follow‐up.…”

Section: Evidence Supporting Lymphamentioning

confidence: 99%

Lymphatic Microsurgical Preventive Healing Approach (LYMPHA) for the prevention of secondary lymphedema

2019

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“…The distribution of all lymphatic vessels in horizontal cross-section must be elucidated first in laboratory animals. 7 , 8 Intradermal or subdermal lymphangiogenesis has been studied morphologically, whereas physiological and pathological lymphatic responses in the deep layers, such as the intramuscular and peri-muscular layers including deep fascia, are still unknown. An adequate animal model to study lymphedema could be very powerful to help reveal the molecular and cellular backgrounds underlying lymphangiogenesis and in developing further treatments for human clinical lymphedema from a novel viewpoint.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Lymphatic Vessels in the Superficial Layer in a Rat Model of a Lymphedematous Response

Matsumoto

Kosaka

Suami

et al. 2018

Plastic and Reconstructive Surgery - Global Open

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Background:The morphologic and histologic behavior of lymphatic vessels in lymphedema has not been well analyzed using laboratory animals. The purpose of the present study was to elucidate the regeneration process of lymphatic vessels after acute lymphedema in a rat model.Methods:The acute lymphedema was induced by an amputation and a replantation surgery on a rat hind limb. Recovery of lymphatic flow was traced using fluorescent lymphography with dye injection. The morphology and number of lymphatic vessels were immunohistochemically detected and quantified in both superficial and deep layers.Results:The swelling was the most severe, and the number of lymphatic vessels in the superficial layer was significantly and maximally increased on postoperative day 3. Backflows and overflows were also detectable in the superficial layer on postoperative day 3. The number of lymphatic vessels had decreased but remained significantly higher than that in the controls on postoperative day 14, when the swelling decreased to the levels in the controls. In contrast, the number of lymphatic vessels in the deep layer showed a tendency toward increased numbers; however, it was not statistically significant on postoperative day 3, 7, or 14.Conclusions:We have obtained solid evidence showing the differential potency of lymphatic vessels between the superficial and the deep layers after temporal lymphedematous induction. Further analysis of lymphedematous responses in animal models could provide new insights into the challenges associated with the clinical treatment of lymphedema.

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A novel pilot animal model for the surgical prevention of lymphedema: the power of optical imaging

Cited by 11 publications

References 31 publications

Immediate lymphatic reconstruction

Immediate lymphatic reconstruction

Lymphatic Microsurgical Preventive Healing Approach (LYMPHA) for the prevention of secondary lymphedema

Enhancement of Lymphatic Vessels in the Superficial Layer in a Rat Model of a Lymphedematous Response

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