A novel photocrosslinked phosphate functionalized Chitosan-Sr5(PO4)2SiO4 composite hydrogels and in vitro biomineralization, osteogenesis, angiogenesis for bone regeneration application

Chen, Yingqi; Udduttula, Anjaneyulu; Xie, Xuelin; Zhou, Meng; Sheng, Weibei; Yu, Fei; Weng, Jian; Wang, Deli; Teng, Bo; Manivasagam, Geetha; Zhang, Jian V.; Ren, Pei‐Gen; Kang, Bin; Zeng, Hui

doi:10.1016/j.compositesb.2021.109057

Cited by 37 publications

(22 citation statements)

References 59 publications

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“…The different concentrations of MgO in these hydrogels were marked as CSMP-MgO (2.5, 5, and 10). As the smaller content of MgO in CSMP solution could not form the hydrogel, the hydrogel with the smaller MgO content (0.5 mg/mL) was formed using chemical cross-linking, as described in our previous study . Briefly, 0.1 g of AM, 2 mg of BIS, and 5 mg of I2959 were added and dissolved in 5 mL of 20 mg/mL CSMP solution.…”

Section: Methodsmentioning

confidence: 99%

“…The commonly used modifying components include hydroxyethyl, carboxymethyl, methacrylate anhydride, and phosphoric acid, among others . Methacrylate chitosan is an attractive option to produce chitosan hydrogels with a low-toxicity photoinitiator under UV irradiation . In addition, phosphorylation of chitosan is also an attractive modification for bone regeneration applications, as phosphate is one of the main inorganic components in the bone matrix and can chelate metallic ions such as calcium and magnesium ions, therefore promoting the formation of mineral bone .…”

Section: Introductionmentioning

confidence: 99%

“…A schematic illustration of the fabrication of this hydrogel is shown in Figure a. Specifically, CS was first grafted with methacrylate anhydride and phosphocreatine (PS) in sequence in a one-step lyophilization method to obtain the phosphate-functionalized methacryloyl chitosan derivate (CSMP), optimizing the synthesis steps compared with our recent studies (two-step lyophilization). , Then MgO nanoparticles were incorporated into CSMP solution to facilitate metal–ligand supramolecular binding between phosphate groups and MgO nanoparticles to fabricate the injectable supramolecular hydrogel. In this hydrogel, phosphocreatine was used as the phosphate-grating molecule, which is a high-energy phosphoric acid and serves as an energy carrier in the human body, with high biosafety and biocompatibility .…”

Section: Introductionmentioning

confidence: 99%

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Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Chen

Sheng

Lin

et al. 2022

ACS Appl. Mater. Interfaces

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Natural polysaccharide (NPH)-based injectable hydrogels have shown great potential for critical-sized bone defect repair. However, their osteogenic, angiogenic, and mechanical properties are insufficient. Here, MgO nanoparticles (NPs) were incorporated into a newly synthesized water-soluble phosphocreatine-functionalized chitosan (CSMP) water solution to form an injectable hydrogel (CSMP-MgO) via supramolecular combination between phosphate groups in CSMP and magnesium in MgO NPs to circumvent these drawbacks of chitosan-based injectable hydrogels. Water-soluble chitosan deviate CSMP was first synthesized by grafting methacrylic anhydride and phosphocreatine into a chitosan chain in a one-step lyophilization process. The phosphocreatine in this hydrogel not only provides sites to combine with MgO NPs to form supramolecular binding but also serves as the reservoir to control Mg 2+ release. As a result, the lyophilized CSMP-MgO hydrogels presented a porous structure with some small holes in the pore wall, and the pore diameters ranged from 50 to 100 μm. The CSMP-MgO injectable hydrogels were restricted from swelling in DI water (lowest swelling ratio was 16.0 ± 1.1 g/g) and presented no brittle failure during compression even at a strain above 85% (maximum compressive strength was 195.0 kPa) versus the control groups (28.0 and 41.3 kPa for CSMP and CSMP-MgO (0.5) hydrogels), with regulated Mg 2+ release in a stable and sustained manner. The CSMP-MgO injectable hydrogels promoted in vitro calcium phosphate (hydroxyapatite (HA) and tetracalcium phosphate (TTCP)) deposition in supersaturated calcium phosphate solution and presented no cytotoxicity to MC3T3-E1 cells; the CSMP-MgO hydrogel promoted MC3T3-E1 cell osteogenic differentiation with upregulation of BSP, OPN, and Osterix osteogenic gene expression and mineralization and HUVEC tube formation. Among them, CSMP-MgO (5) presented most of these properties. Moreover, this hydrogel (CSMP-MgO ( 5)) showed an excellent ability to promote new bone formation in critical-sized calvarial defects in rats. Thus, the CSMP-MgO injectable hydrogel shows great promise for bone regeneration.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Chen

Sheng

Lin

et al. 2022

ACS Appl. Mater. Interfaces

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“…Also, they accelerated endothelial tube formation with enhanced capillary lengths, branch points, and angiogenic gene vegf expression. 98 Achieving optimized conditions for the biomechanical activity, bioactivity, and osteoconductivity for inducing bone tissue regeneration is very challenging and should be considered while designing the biomaterial composition. In this case, polyhedral oligomeric silsesquioxane (POSS) was considered an effective NPs to impart physical-chemical crosslinking and enhanced mineralized ability by silicon in POSS NPs bonding to calcium ions (Ca 2+ ).…”

Section: Reviewmentioning

confidence: 99%

“…Also, they accelerated endothelial tube formation with enhanced capillary lengths, branch points, and angiogenic gene vegf expression. 98…”

Section: Composite Hydrogels-encouraging Coupled Angiogenesis–osteoge...mentioning

confidence: 99%

Manufacturing functional hydrogels for inducing angiogenic–osteogenic coupled progressions in hard tissue repairs: prospects and challenges

et al. 2022

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Piezoelectric Bioactive Glasses Composite Promotes Angiogenesis by the Synergistic Effect of Wireless Electrical Stimulation and Active Ions

Zhang

Yao

et al. 2023

Adv Healthcare Materials

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Insufficient angiogenesis frequently occurs after the implantation of orthopedic materials, which greatly increases the risk of bone defect reconstruction failure. Therefore, the development of bone implant with improved angiogenic properties is of great importance. Mimicking the extracellular matrix clues provides a more direct and effective strategy to modulate angiogenesis. Herein, inspired by the bioelectrical characteristics of the bone microenvironment, a piezoelectric bioactive glasses composite (P‐KNN/BG) based on the incorporation of polarized potassium sodium niobate is constructed, which could effectively promote angiogenesis. It is found that P‐KNN/BG has exceptional wireless electrical stimulation performance and sustained active ions release. In vitro cell experiments reveal that P‐KNN/BG enhances endothelial cell adhesion, migration, and differentiation via activating the eNOS/NO signaling pathway, which might be contributed to cell membrane hyperpolarization induced by wireless electrical stimulation increase the influx of active ions into the cells. In vivo chick chorioallantoic membrane experiment demonstrates that P‐KNN/BG shows excellent pro‐angiogenic capacity and biocompatibility. This work broadens the current understanding of bioactive materials with bionic electrical properties, which brings new insights into the clinical treatment of bone defect repair.

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A novel photocrosslinked phosphate functionalized Chitosan-Sr5(PO4)2SiO4 composite hydrogels and in vitro biomineralization, osteogenesis, angiogenesis for bone regeneration application

Cited by 37 publications

References 59 publications

Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Magnesium Oxide Nanoparticle Coordinated Phosphate-Functionalized Chitosan Injectable Hydrogel for Osteogenesis and Angiogenesis in Bone Regeneration

Manufacturing functional hydrogels for inducing angiogenic–osteogenic coupled progressions in hard tissue repairs: prospects and challenges

Piezoelectric Bioactive Glasses Composite Promotes Angiogenesis by the Synergistic Effect of Wireless Electrical Stimulation and Active Ions

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