A Novel Peptide from Soybean Protein Isolate Significantly Enhances Resistance of the Organism under Oxidative Stress

Abstract

Recent studies have indicated that protein hydrolysates have broad biological effects. In the current study we describe a novel antioxidative peptide, FDPAL, from soybean protein isolate (SPI). The aim of this study was to purify and characterize an antioxidative peptide from SPI and determine its antioxidative mechanism. LC–MS/MS was used to isolate and identify the peptide from SPI. The sequence of the peptide was determined to be Phe-Asp-Pro-Ala-Leu (FDPAL, 561 Da). FDPAL can cause significant enhancement of resistance to oxidative stress both in cells as well as simple organisms. In Caenorhabditis elegans (C. elegans), FDPAL can up-regulate the expression of certain genes associated with resistance. The antioxidant activity of this peptide can be attributed to the presence of a specific amino acid sequence. Results from our work suggest that FDPAL can facilitate potential applications of proteins carrying this sequence in the nutraceutical, bioactive material and clinical medicine areas, as well as in cosmetics and health care products.

Preparation of soy protein hydrolysates with antioxidant activity by using peptidases from latex of Maclura pomifera fruits

“…Likewise, the percentage of antioxidant amino acids was calculated for two peptides found in previous investigations. Thus, the peptides SHCMN (Lin, Liang, Li, Xing, & Yuan, 2016), and FDPAL (Ma et al, 2016) from soybean protein both presented a percentage of antioxidant amino acids of 60%.…”

Antioxidant action and protective and reparative effects of lentinan on oxidative damage in HaCaT cells

“…With advancements in the modern sciences, cosmetics have advanced from basic skin care products focusing on the cleaning and moisturizing of the skin to functional cosmetics focusing on delaying of skin aging and improving the skin appearance. Recently, more studies have focused on plant‐derived natural biological macromolecules such as proteins, polypeptides, and polyoses with lentinan being one of the latter . Lentinan is reported to have good skin care activity such as beautifying, whitening, and moisturizing of the skins as well as showing antisensitive and anti‐microbial effects; however, few studies have investigated the antiaging effects of lentinan on the skin …”

Peptides Derived from Foods as Supportive Diet Components in the Prevention of Metabolic Syndrome

“…The hydrolysis of a soy protein isolate with Alcalase (Novo Nordisk, Shenyang Biochemical Processing Co. Ltd., China) was shown to generate the FDPAL peptide which antioxidative potential was confirmed in vitro (by determining its capability to scavenge free radicals), as well as in vivo (reduction of the accumulation of ROS and lipofuscin, up‐regulation of the SOD expression). The FDPAL improved the survival rate of Hela cells and Caenorhabditis elegans under oxidative stress (Ma and others ). Xu and others () hydrolyzed defatted soybean meal with Neutrase (Novo Nordisk, Bagsvaerd, Denmark) to produce the EEQEWPRKEEK peptide that exhibited free radical scavenging activity against DPPH and ABTS.…”