A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish

Multifunctional peptide‐based fluorescent chemosensor for detection of Hg2+, Cu2+ and S2− ions

“…Several common anions such as Ac − , F − , Cl − , ClO 4 − , Br − , I − , PO 4 3− , CO 3 2− , NO 2 − , NO 3 − and, especially, other sulfur‐containing ions including SCN − , SO 4 2− , SO 3 2− and S 2 O 3 2− did not affect the spectra of DP‐5‐Hg and DP‐5‐Cu systems, inferring that the two systems have selective responses for S 2− . Addition of S 2− resulted in decrease in fluorescence emission intensity rather than the regeneration of fluorescence intensity reported by Tang and colleagues for the peptide–Cu system sensor for detection of S 2− . Differences in these two fluorescent quenching behaviors could be attributed to S 2− induction of Cu 2+ release from the peptide–Cu complex and resulted in formation of CuS for the latter; S 2− did not induce Cu 2+ release from the peptide–Cu complex and resulted in the formation of a ternary complex for the former.…”

“…This fluorescent peptide probe can differentiate Cu 2+ and Hg 2+ by different response types, performing a turn‐on response to Hg 2+ and a turn‐off response to Cu 2+ . Tang and colleagues reported two novel peptide fluorescent chemosensors that exhibited fluorescence quenching responses for Cu 2+ and Hg 2+ , and that distinguished these two heavy metal ions by the same response type …”

“…The design of effective probes for monitoring S 2− is significant because abnormal levels of S 2− have been associated with increased risk of many diseases, however peptide‐based sensors for S 2− detection are rarely reported. Recently, Tang and colleagues described a novel peptide‐based fluorescence sensor for detection of Cu(II) and S 2− . These chemosensors display an ‘on–off–on’ fluorescence response change on the addition of Cu 2+ and S 2− to aqueous solution, however these sensors were often affected by Hg(II) when selectively detecting Cu(II) .…”

“…Recently, Tang and colleagues described a novel peptide‐based fluorescence sensor for detection of Cu(II) and S 2− . These chemosensors display an ‘on–off–on’ fluorescence response change on the addition of Cu 2+ and S 2− to aqueous solution, however these sensors were often affected by Hg(II) when selectively detecting Cu(II) . In this study, we synthesized a new multifunctional chemosensor based on a pentapeptide, dansyl‐Gly‐His‐Gly‐Gly‐Trp‐COOH (D‐P5), to monitor Hg(II) and Cu(II) and especially S 2− .…”

Competitive and noncompetitive immunoassays for the detection of benzothiostrobin using magnetic nanoparticles and fluorescein isothiocyanate-labeled peptides

“…Synthetic peptides are relatively cheap materials that can be easily modified to possess a detection or binding element [15]. Previous studies have constructed peptides bound to biotin [16][17][18], fluorescein isothiocyanate (FITC) [19,20], and gold nanoparticles [21]. Using these reagents, the assay sensitivities and peptide functionalities are not affected [16,22], and the detection process can be completed within fewer steps and in less time [15,[18][19][20].…”