A Novel Pbx family member expressed during early zebrafish embryogenesis forms trimeric complexes with Meis3 and Hoxb1b

Vlachakis, Nikolaos; Ellstrom, Deborah R.; Sagerström, Charles G.

doi:10.1002/(sici)1097-0177(200001)217:1<109::aid-dvdy10>3.0.co;2-8

Cited by 52 publications

(29 citation statements)

References 45 publications

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“…In control embryos, a pan-zebrafish-Pbx antibody (Pöpperl et al, 2000) identified ubiquitous nuclear Pbx expression in early paraxial mesoderm, and ubiquitous Pbx expression was maintained during somite development and early muscle differentiation (see Fig. S1A-C in the supplementary material), consistent with prior reports of broad pbx mRNA expression during early zebrafish development (Pöpperl et al, 2000;Vlachakis et al, 2000;Waskiewicz et al, 2002), and confirming that Pbx proteins are present at the right time and place to potentially regulate early muscle development. By contrast, Pbx protein expression was essentially absent in pbx2-MO; pbx4-MO embryos (see Fig.…”

Section: Pbx and Myod Are Required For Activation Of Myog Expressionsupporting

confidence: 86%

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Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation

et al. 2007

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The basic helix-loop-helix (bHLH) transcription factor Myod directly regulates gene expression throughout the program of skeletal muscle differentiation. It is not known how a Myod-driven myogenic program is modulated to achieve muscle fiber-type-specific gene expression. Pbx homeodomain proteins mark promoters of a subset of Myod target genes, including myogenin (Myog); thus, Pbx proteins might modulate the program of myogenesis driven by Myod. By inhibiting Pbx function in zebrafish embryos, we show that Pbx proteins are required in order for Myod to induce the expression of a subset of muscle genes in the somites. In the absence of Pbx function, expression of myog and of fast-muscle genes is inhibited, whereas slow-muscle gene expression appears normal. By knocking down Pbx or Myod function in combination with another bHLH myogenic factor, Myf5, we show that Pbx is required for Myod to regulate fast-muscle, but not slow-muscle, development. Furthermore, we show that Sonic hedgehog requires Myod in order to induce both fast-and slow-muscle markers but requires Pbx only to induce fast-muscle markers. Our results reveal that Pbx proteins modulate Myod activity to drive fast-muscle gene expression, thus showing that homeodomain proteins can direct bHLH proteins to establish a specific cell-type identity.

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Section: Pbx and Myod Are Required For Activation Of Myog Expressionsupporting

confidence: 86%

“…Pbx4 and Meis3 pCS2 expression constructs were as previously described (Pöpperl et al, 2000;Vlachakis et al, 2000).…”

Section: Emsamentioning

confidence: 99%

Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation

et al. 2007

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“…Nonetheless, Waskiewicz et al (2001) showed in their Figure 1 a hindbrain pattern that is nearly coincident with that found by us in chicken, with a rostral boundary at the rh1-rh2 limit, as deduced from comparison with krox20 expression in rh3-rh5. Moreover, zebrafish meis3 is expressed in caudal rhombomeres, stopping rostrally at the rh3/rh4 boundary (Vlachakis et al, 2000(Vlachakis et al, , 2001Waskiewicz et al, 2001). In Xenopus, Xmeis3 expression at early tail bud stage extends from rh2 to the caudalmost rhombomeres (Salzberg et al, 1999).…”

Section: Meis Gene Expression From Neural Tube Closure To Hh20 Stagesmentioning

confidence: 99%

Distinct and redundant expression and transcriptional diversity of MEIS gene paralogs during chicken development

Sánchez-Guardado

Irimia

Sánchez-Arrones

et al. 2011

Developmental Dynamics

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Members of the Meis family of TALE homeobox transcription factors are involved in many processes of vertebrate development and morphogenesis, showing extremely complex transcriptional and spatiotemporal expression patterns. In this work, we performed a comprehensive study of chicken Meis genes using multiple approaches. First, we assessed whether the chicken genome contains a Meis3 ortholog or harbors only two Meis genes; we gathered several lines of evidence pointing to a specific loss of the Meis3 ortholog in an early ancestor of birds. Next, we studied the transcriptional diversity generated from chicken Meis genes through alternative splicing during development. Finally, we performed a detailed analysis of chick Meis1/ 2 expression patterns during early embryogenesis and organogenesis. We show that the expression of both Meis genes begins at the gastrulation stage in the three embryonic layers, presenting highly dynamic patterns with overlapping as well as distinct expression domains throughout development. Developmental Dynamics 240:1475-1492, 2011. V C 2011 Wiley-Liss, Inc.Key words: TALE family; alternative splicing; ectoderm; mesoderm; endoderm; central nervous system; sensory organs; visceral development; gut Accepted 17 January 2011 Developmental DynamicsABBREVIATIONS A atrium aip anterior intestinal portal as alternative splicing av allantoic vesicle ba branchial arches bd bile duct C conotruncus cl cloaca cnp caudal neural plate co coelom cp cardiac primordium D diencephalon da dorsal aorta dh dorsal horn dm dermomyotome dp dorsal pancreas du duodenum ect ectoderm ep ephiphysis end endoderm Eo-PmC extraocular pre-muscle cluster FB forebrain fg foregut fp floor plate Hn Hensen node HB hindbrain Hyp hypothalamus inf infundibulum isth isthmus l lens ltl larynx-trachea-lug bud lpm lateral plate mesoderm lp lens placode lv liver lb lung bud MB midbrain Mes mesencephalon mes mesoderm mg mid-gut mn mesonephric system mp mandibular process MRB mesencephalic-rhombencephalic boundary nep nephrotome nf neural fold np neural plate nr neural retina nt notochord oc otic cup oe oesophagus of optic furrow omv omphalomensenteric vena os optic stalk ot otocyst ov optic vesicle p prosomere p1 prosomere 1 p2 prosomere 2 p3 prosomere 3 pc pericardial cavity pe pigmented epithelium pf primitive fold pg primitive groove ph pharynx pm precardiac mesoderm POA preoptic area pp prechordal plate ps primitive streak psm primitive streak mesoderm PT pretectum Rh rhombencephalon rh rhombomere RhA1 rhombomere A1 rnp rostral neural plate Rp Rathke's pouch rp roof plate SC spinal cord scl sclerotome sm somatic mesoderm smp somatopleure som somite SP secondary prosencephalon sp segmental plate spm splanchnic mesoderm spp splanchnopleure st stomach sub.p subcephalic pocket sv sinus venosus tb tail bud td thyro-glossal duct Tel telencephalon TH thalamus Tv telencephalic vesicle V.n trigeminal ganglion V ventricle WGS whole genome shotgun zli zona limitans intrathalamica

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“…The vertebrate Pbx and Meis genes encode divergent homeodomain-containing proteins of the TALE (three amino acid Loop extension) class, which are essential DNA binding partners for the Hox proteins (reviewed by Mann and Affolter, 1998). Two zebrafish pbx genes and five zebrafish meis genes are expressed in the presumptive hindbrain during the period when Hox genes are specifying segment identity (Pöpperl et al, 2000;Vlachakis et al, 2000;Waskiewicz et al, 2001;Zerucha and Prince, 2001). Eliminating the zygotic function of one of these, Pbx4, as occurs in the lazarus (lzr)/pbx4 mutant, mimics the phenotypes associated with loss of several different Hox genes in the mouse (Pöpperl et al, 2000;K.L.…”

Section: Pbx Genes: Revealing Conserved Roles For Hox Genes In Specifmentioning

confidence: 99%

“…The other class of Hox partners is the TALE-homeobox containing Meis proteins, which have more tightly regulated and dynamic expression during hindbrain development (Vlachakis et al, 2000;Waskiewicz et al, 2001;Zerucha and Prince, 2001;Sagerström et al, 2001;Fig. 8B).…”

Section: Meis Genes: Multifunctional Regulators Of Hox Activitymentioning

confidence: 99%

Constructing the hindbrain: Insights from the zebrafish

Moens¹,

Prince²

2002

Developmental Dynamics

195

172

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The hindbrain is responsible for controlling essential functions such as respiration and heart beat that we literally do not think about most of the time. In addition, cranial nerves projecting from the hindbrain control muscles in the jaw, eye, and face, and receive sensory input from these same areas. In all vertebrates that have been studied, the hindbrain passes through a segmented phase shortly after the neural tube has formed, with a series of seven bulges-the rhombomeres-forming along the anterior-posterior extent of the neural tube. Our current understanding of vertebrate hindbrain development comes from integrating data from several model systems. Work on the chick has helped us to understand the cell biology of the rhombomeres, whereas the power of mouse molecular genetics has allowed investigation of the molecular mechanisms underlying their development. This review focuses on the special insights that the zebrafish system has provided to our understanding of hindbrain development. As we will discuss, work in the zebrafish has elucidated inductive events that specify the presumptive hindbrain domain and has identified genes required for hindbrain segmentation and the specification of segment identities.

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A Novel Pbx family member expressed during early zebrafish embryogenesis forms trimeric complexes with Meis3 and Hoxb1b

Cited by 52 publications

References 45 publications

Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation

Pbx homeodomain proteins direct Myod activity to promote fast-muscle differentiation

Distinct and redundant expression and transcriptional diversity of MEIS gene paralogs during chicken development

Constructing the hindbrain: Insights from the zebrafish

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