A novel pathogenic bacteria (<i>Vibrio fortis</i>) causing enteritis in cultured seahorses, <i>Hippocampus erectus</i> Perry, 1810

Wang, X; Zhang, Y; Qin, Geng; Luo, Wei; Lin, Qiang

doi:10.1111/jfd.12411

Cited by 48 publications

(44 citation statements)

References 23 publications

(46 reference statements)

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“…It has been treated as vulnerable or endangered in the Red List of Threatened Species (IUCN 2015) [7]. Moreover, the lined seahorse is easily domesticated for breeding, and it has become a popular and commercially important ingredient for traditional Chinese medicine in China [8–12]. In order to study the evolutionary history of the lined seahorse and improve its aquaculture yield, we are trying to develop genomic resources for assisted selection in genetic breeding.…”

Section: Data Descriptionmentioning

confidence: 99%

Draft genome of the lined seahorse, Hippocampus erectus

Lin

Qiu

et al. 2017

GigaScience

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Background: The lined seahorse, Hippocampus erectus, is an Atlantic species and mainly inhabits shallow sea beds or coral reefs. It has become very popular in China for its wide use in traditional Chinese medicine. In order to improve the aquaculture yield of this valuable fish species, we are trying to develop genomic resources for assistant selection in genetic breeding. Here, we provide whole genome sequencing, assembly, and gene annotation of the lined seahorse, which can enrich genome resource and further application for its molecular breeding. Findings: A total of 174.6 Gb (Gigabase) raw DNA sequences were generated by the Illumina Hiseq2500 platform. The final assembly of the lined seahorse genome is around 458 Mb, representing 94% of the estimated genome size (489 Mb by k-mer analysis). The contig N50 and scaffold N50 reached 14.57 kb and 1.97 Mb, respectively. Quality of the assembled genome was assessed by BUSCO with prediction of 85% of the known vertebrate genes and evaluated using the de novo assembled RNA-seq transcripts to prove a high mapping ratio (more than 99% transcripts could be mapped to the assembly). Using homology-based, de novo and transcriptome-based prediction methods, we predicted 20 788 protein-coding genes in the generated assembly, which is less than our previously reported gene number (23 458) of the tiger tail seahorse (H. comes). Conclusion: We report a draft genome of the lined seahorse. These generated genomic data are going to enrich genome resource of this economically important fish, and also provide insights into the genetic mechanisms of its iconic morphology and male pregnancy behavior.

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Section: Data Descriptionmentioning

confidence: 99%

Draft genome of the lined seahorse, Hippocampus erectus

Lin

Qiu

et al. 2017

GigaScience

View full text Add to dashboard Cite

show abstract

“…), and major mortality of cultured seahorses is firstly reported in the early 2014 (Wang et al . ), and since then, the same epidemic sign is also found in all seahorse farms along the China's coast.…”

Section: Mortality and Food Intake Of The Lined Seahorses Hippocampusmentioning

confidence: 53%

“…PCR is targeted to 16S rRNA gene sequencing, and several housekeeping genes, including GapA , rpoA and pyrH, were analysed based on the methods by Wang et al . () and Gong et al . ().…”

Section: Mortality and Food Intake Of The Lined Seahorses Hippocampusmentioning

confidence: 93%

“…This process is followed the method by Wang et al . (). We continued to study which pathogenic bacteria were the causative agent of the diseases in seahorses.…”

Section: Mortality and Food Intake Of The Lined Seahorses Hippocampusmentioning

confidence: 97%

“…) and V. fortis (Wang et al . ) have been identified as the pathogenic bacteria from different seahorse species. In contrast, the present study demonstrated that V. harveyi could be able to induce a heavy loss of production during commercial seahorse culture, and V. harveyi can directly and seriously destroy the seahorse kidney without any other signs in other tissues, so once the cultured seahorses were infected by V. harveyi , they will die sooner or later.…”

Section: Mortality and Food Intake Of The Lined Seahorses Hippocampusmentioning

confidence: 99%

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