2019
DOI: 10.1002/jbmr.3720
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A Novel Osteogenic Cell Line That Differentiates Into GFP-Tagged Osteocytes and Forms Mineral With a Bone-Like Lacunocanalicular Structure

Abstract: Osteocytes, the most abundant cells in bone, were once thought to be inactive, but are now known to have multifunctional roles in bone, including in mechanotransduction, regulation of osteoblast and osteoclast function and phosphate homeostasis. Because osteocytes are embedded in a mineralized matrix and are challenging to study, there is a need for new tools and cell models to understand their biology. We have generated two clonal osteogenic cell lines, OmGFP66 and OmGFP10, by immortalization of primary bone … Show more

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Cited by 40 publications
(37 citation statements)
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“…Importantly, PTHrP, much like PTH, displays anti-apoptotic features in osteoblasts and osteocytes 132 134 . Similarly, it also was corroborated that many of the osteocyte-related genes, such as SOST, DMP-1, were downregulated by PTH in an expected manner in OmGFP66 cells (immortalized osteogenic cell lines) derived from primary osteocytes of transgenic mice (DMP1-promoter) 29 . Based on these findings, it is demonstrated that the PTHrP/PTH1R pathway functions as an essential pathway in preserving the osteocytic vitality upon mechanical stimulus (Fig.…”
Section: The Key Molecular Mechanisms In Preserving the Osteocytic VImentioning
confidence: 68%
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“…Importantly, PTHrP, much like PTH, displays anti-apoptotic features in osteoblasts and osteocytes 132 134 . Similarly, it also was corroborated that many of the osteocyte-related genes, such as SOST, DMP-1, were downregulated by PTH in an expected manner in OmGFP66 cells (immortalized osteogenic cell lines) derived from primary osteocytes of transgenic mice (DMP1-promoter) 29 . Based on these findings, it is demonstrated that the PTHrP/PTH1R pathway functions as an essential pathway in preserving the osteocytic vitality upon mechanical stimulus (Fig.…”
Section: The Key Molecular Mechanisms In Preserving the Osteocytic VImentioning
confidence: 68%
“…Trigger the generation of pro-inflammatory and pro-osteoclastic factors via positive feedback loop [109][110][111][112]114 FADD Trigger a caspase cascade; induce GCs-induced apoptosis 6,118 Sclerostin Promote osteocyte cells death upon unloading; inhibit bone formation 53,107,108 BNIP3 Promote cell death during hypoxia 60,62 CCN2 Promote osteocyte apoptosis upon excess mechanical stress 33,69 TNF-α Stimulate osteocyte apoptosis upon inflammation and cancer 92,115,116 Caspase-3 Regulate osteocyte apoptosis via physical interactions in mechanistic stimulus 27,127 CTSK Breakdown the bone matrix adjacent to the osteocyte; increase the size of the osteocyte lacunae and mineralization decrease vitality of osteocytes 23 DMP-1 Regulate osteocyte formation and phosphate homeostasis; involve in osteocytic apoptosis 29,84,154 Pyk2 Promote GCs-induced osteocytic apoptosis via focal adhesion 82 Panx-1 Promote fatigue-induced osteocytic apoptosis 160 Anti-apoptotic function SOD2 Suppress aging and loss of bone mass; decrease degeneration of the osteocyte LCN 24,61 AMPK Protects against Hcy-induced osteocyte apoptosis 96,101,102 NO Maintain osteocytic vitality by pulsatile fluid flow 44,54,100…”
Section: Hmgb1mentioning
confidence: 99%
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“…The specific role for FGF9 in skeletal muscle differentiation is elusive. Recent studies from our groups showed that FGF9 mRNA expression is highly enriched in osteocytes in the OmGFP66 osteogenic cell line as compared to the osteoblast population [40,41]. We have reasoned that this molecule could be functioning as an osteokine based on our observations (Huang & Brotto, Unpublished Results) that fibroblast-conditioned media can inhibit C2C12 differentiation.…”
Section: Introductionmentioning
confidence: 91%
“…The major expectation from these cell lines is that they develop osteocytic‐like cells within a mature bone matrix, form lacunocanalicular structures, and allow 3D organization of osteocytes. In this issue of the Journal of Bone and Mineral Research ( JBMR ), Wang and colleagues report a novel cell line that meets most of these morphological criteria. Two novel cell lines were derived from osteoblast‐rich calvarial digests from mice, using membrane‐bound GFP (AcGFP‐mem; Clontech) driven by the DMP1 promoter as selection mechanism.…”
mentioning
confidence: 99%