A novel one step process for cellulose fermentation using mesophilic cellulolytic and glycolytic Clostridia

Petitdemange, E.; Fond, O.; Caillet, François; Petitdemange, H.

doi:10.1007/bf00132170

Cited by 38 publications

(26 citation statements)

References 6 publications

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“…Conversely, when strain N1-4 was added to medium in which C. thermocellum had been incubated at 60°C for a minimum of 24 h, all cocultures incubated at 30°C produced butanol from crystalline cellulose. These findings substantiated those of a previous study (24) in which cellulose degradation was optimal only when the butanol-producing strain was added to the growth medium after the strain capable of utilizing cellulose, C. thermocellum, had been cultured on the same medium. Thus, the optimal expression of cellulosome compo- nents was essential for producing butanol from crystalline cellulose in both the present and in the previous study.…”

Section: Discussionsupporting

confidence: 90%

“…5a and b). As reported, C. acetobutylicum produced low levels of butanol (24,25,34), but butanol production by C. beijerinckii was 26% of that obtained using strain N1-4. The amount of butanol produced by the three strains was notably similar when the strains were separately cultivated in CGM containing 40 g/liter glucose (Fig.…”

Section: Resultssupporting

confidence: 74%

“…An example of this type of system is cocultured C. acetobutylicum and the cellulolytic Clostridium cellulolyticum or Clostridium thermocellum (24,25,34). The high rates of cellulose hydrolysis achieved by these cellulolytic clostridia can be attributed to the presence of a large, cell surface-bound protein complex referred to as the cellulosome (5,11,32).…”

mentioning

confidence: 99%

“…The high rates of cellulose hydrolysis achieved by these cellulolytic clostridia can be attributed to the presence of a large, cell surface-bound protein complex referred to as the cellulosome (5,11,32). Although these coculture systems produced small quantities of butanol using simple carbon sources, they mainly produce butyrate (24,25). Even so, butanol production using a coculture system requires butyric acid to induce the solventogenic phase of C. acetobutylicum (34).…”

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confidence: 99%

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Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4

Nakayama

Kiyoshi

Kadokura

et al. 2011

Appl Environ Microbiol

111

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We investigated butanol production from crystalline cellulose by cocultured cellulolytic Clostridium thermocellum and the butanol-producing strain, Clostridium saccharoperbutylacetonicum (strain N1-4). Butanol was produced from Avicel cellulose after it was incubated with C. thermocellum for at least 24 h at 60°C before the addition of strain N1-4. Butanol produced by strain N1-4 on 4% Avicel cellulose peaked (7.9 g/liter) after 9 days of incubation at 30°C, and acetone was undetectable in this coculture system. Less butanol was produced by cocultured Clostridium acetobutylicum and Clostridium beijerinckii than by strain N1-4, indicating that strain N1-4 was the optimal strain for producing butanol from crystalline cellulose in this coculture system.

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Section: Discussionsupporting

confidence: 90%

Section: Resultssupporting

confidence: 74%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4

Nakayama

Kiyoshi

Kadokura

et al. 2011

Appl Environ Microbiol

111

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“…Petitdemange et al 125,126 and Fond et al 127 tried to use mesophilic cocultures of cellulolytic (C. cellulolyticum H10) and glycolytic (C. acetobutylicum) clostridial strains for direct fermentation of cellulose. However, the major fermentation product was acid (butyric acid), with a small amount of acetone, ethanol, and butanol.…”

Section: Coculturesmentioning

confidence: 99%

Biobutanol: the outlook of an academic and industrialist

et al. 2013

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The gradual shift of transportation fuels from oil based fuels to the alternative fuel resources and worldwide demand for energy has been the impetus for research to produce alcohol biofuels from renewable resources. Current bioethanol and biodiesel can, however, not cover an increasing demand for biofuels. Hence, there is an extensive need for advanced biofuels with superior fuel properties. The present review is focused on the developments of biobutanol, which is regarded to be superior to bioethanol in terms of energy density and hygroscopicity. Although acetone-butanolethanol (ABE) fermentation is one of the oldest large-scale fermentation processes, butanol yield by anaerobic fermentation remains sub-optimal. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, low product yield, product toxicity to production strain, multiple end-products and downstream processing of alcohol mixtures.Metabolic engineering provides a means for fermentation improvements. Different strategies are employed in the metabolic engineering of Clostridia that aim to enhance the solvent production, improve selectivity for butanol production, and increase the tolerance of Clostridia to solvents. The introduction and expression of a non-clostridial butanol producing pathway in E. coli is most promising strategy for butanol biosynthesis. Several rigorous kinetic and physiological models for fermentation have been formulated, which form useful tool for optimization of the process. Due to the lower butanol titers in the fermentation broth, simultaneous fermentation and product removal techniques have been developed to improve production economics. With the use of new strains, inexpensive substrates, and superior reactor designs, economic ABE fermentation may further attract an attention of researchers all over the world. The present review is attempting to provide an overall outlook on discoveries and strategies that are being developed for commercial n-butanol production.

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Genome‐scale metabolic modeling of a clostridial co‐culture for consolidated bioprocessing

Salimi

Zhuang

Mahadevan

2010

Biotechnology Journal

107

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An alternative consolidated bioprocessing approach is the use of a co-culture containing cellulolytic and solventogenic clostridia. It has been demonstrated that the rate of cellulose utilization in the co-culture of Clostridium acetobutylicum and Clostridium cellulolyticum is improved compared to the mono-culture of C. cellulolyticum, suggesting the presence of syntrophy between these two species. However, the metabolic interactions in the co-culture are not well understood. To understand the metabolic interactions in the co-culture, we developed a genome-scale metabolic model of C. cellulolyticum comprising of 431 genes, 621 reactions, and 603 metabolites. The C. cellulolyticum model can successfully predict the chemostat growth and byproduct secretion with cellulose as the substrate. However, a growth arrest phenomenon, which occurs in batch cultures of C. cellulolyticum at cellulose concentrations higher than 6.7 g/L, cannot be predicted by dynamic flux balance analysis due to the lack of understanding of the underlying mechanism. These genome-scale metabolic models of the pure cultures have also been integrated using a community modeling framework to develop a dynamic model of metabolic interactions in the co-culture. Co-culture simulations suggest that cellobiose inhibition cannot be the main factor that is responsible for improved cellulose utilization relative to mono-culture of C. cellulolyticum.

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A novel one step process for cellulose fermentation using mesophilic cellulolytic and glycolytic Clostridia

Cited by 38 publications

References 6 publications

Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4

Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4

Biobutanol: the outlook of an academic and industrialist

Genome‐scale metabolic modeling of a clostridial co‐culture for consolidated bioprocessing

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