A novel one-step Helicobacter pylori saliva antigen test

Yang, Bi-Ling; Yeh, Chun Chang; Kwong, Wei-Gang; Lee, Shou‐Dong

doi:10.1016/j.jcma.2014.11.004

Cited by 16 publications

(8 citation statements)

References 20 publications

(26 reference statements)

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“…Classically, non-invasive tests for H. pylori detection include stool antigen assays, serology and the frequently used urea breath test (UBT)[68,69]. Antigen tests have been widely used for H. pylori detection in clinical specimens like gastric juice, saliva, urine and stool[70-72]. However, antigen detection methods, may suffer from poor specificity and sensitivity[70,71,73].…”

Section: Current Helicobacter Pylori Diagnosticsmentioning

confidence: 99%

“…Antigen tests have been widely used for H. pylori detection in clinical specimens like gastric juice, saliva, urine and stool[70-72]. However, antigen detection methods, may suffer from poor specificity and sensitivity[70,71,73]. Different stool antigen tests have been developed to detect H. pylori in stool specimens with a sensitivity and specificity of 85% to 95%[17].…”

Section: Current Helicobacter Pylori Diagnosticsmentioning

confidence: 99%

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Review of current diagnostic methods and advances inHelicobacter pyloridiagnostics in the era of next generation sequencing

Pohl

Keller

Bordier

et al. 2019

WJG

104

109

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Helicobacter pylori ( H. pylori ) infection is highly prevalent in the human population and may lead to severe gastrointestinal pathology including gastric and duodenal ulcers, mucosa associated tissue lymphoma and gastric adenocarcinoma. In recent years, an alarming increase in antimicrobial resistance and subsequently failing empiric H. pylori eradication therapies have been noted worldwide, also in many European countries. Therefore, rapid and accurate determination of H. pylori ’s antibiotic susceptibility prior to the administration of eradication regimens becomes ever more important. Traditionally, detection of H.pylori and its antimicrobial resistance is done by culture and phenotypic drug susceptibility testing that are cumbersome with a long turn-around-time. Recent advances in diagnostics provide new tools, like real-time polymerase chain reaction (PCR) and line probe assays, to diagnose H. pylori infection and antimicrobial resistance to certain antibiotics, directly from clinical specimens. Moreover, high-throughput whole genome sequencing technologies allow the rapid analysis of the pathogen’s genome, thereby allowing identification of resistance mutations and associated antibiotic resistance. In the first part of this review, we will give an overview on currently available diagnostic methods for detection of H. pylori and its drug resistance and their implementation in H. pylori management. The second part of the review focusses on the use of next generation sequencing technology in H. pylori research. To this end, we conducted a literature search for original research articles in English using the terms “ Helicobacter ”, “transcriptomic”, “transcriptome”, “next generation sequencing” and “whole genome sequencing”. This review is aimed to bridge the gap between current diagnostic practice (histology, rapid urease test, H. pylori culture, PCR and line probe assays) and new sequencing technologies and their potential implementation in diagnostic laboratory settings in order to complement the currently recommended H. pylori management guidelines and subsequently improve public health.

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Section: Current Helicobacter Pylori Diagnosticsmentioning

confidence: 99%

Section: Current Helicobacter Pylori Diagnosticsmentioning

confidence: 99%

Review of current diagnostic methods and advances inHelicobacter pyloridiagnostics in the era of next generation sequencing

Pohl

Keller

Bordier

et al. 2019

WJG

104

109

View full text Add to dashboard Cite

show abstract

“…Helicobacter pylori ( H. pylori ) infection is probably one of the most common chronic bacterial infections worldwide [ 1 ]. The stomach is the primary site of H. pylori infection, including chronic active gastritis, peptic ulcer, gastric adenocarcinoma and type B low-grade mucosa-associated lymphoid tissue lymphoma [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection

Nasif

Mukhtar

Eldein

et al. 2016

Diabetol Metab Syndr

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BackgroundHelicobacter pylori (H. pylori) infection is reported to be associated with various extragastrointestinal conditions such as insulin resistance, diabetes mellitus and metabolic syndrome. H. pylori infection and type 2 diabetes mellitus (T2DM) are associated with oxidative stress, this cross-relation between H. pylori induced infection in T2DM and oxidative damage is still debated. Thus, the question arises whether an increase in the serum level of 8-OHdG and Ox-LDL will occurs in patients with T2DM infected H. pylori; this will be through determination and compare frequency of H. pylori infection in T2DM and non-diabetic patients.Methods100 patients presented with history of epigastric discomfort for more than 1 month; 50 patients with T2DM and 50 non-diabetics. Anti-H. pylori IgG using ELISA, fasting and postprandial glucose level, glycated hemoglobin (HbA1c) and body mass index (BMI) was calculated. Serum 8-OHdG and Ox-LDL was measured using ELISA for the 100 patients and 50 control subject.ResultsRates of H. pylori infection of T2DM and non-diabetic were 66 and 58 %, respectively, (p = 0.001). H. pylori IgG antibody was not correlated with HbA1c either in T2DM (p = 0.06) or non-diabetic (p = 0.25). Serum 8-OHdG level in T2DM with positive H. pylori infection showed a significant difference compared to non-diabetics with positive H. pylori infection (p = 0.001) and higher than that in T2DM with negative H. pylori. A correlation between 8-OHdG concentration and HbA1c in T2DM patients infected with H. pylori was observed (r = 0.39, p = 0.02). Serum Ox-LDL level in T2DM with positive H. pylori infection showed a significant difference compared to diabetics with both negative H. pylori infection and in non-diabetics with positive H. pylori infection (p = 0.001).ConclusionsIncreased levels of oxidative DNA damage (8-OHdG) and Ox-LDL suggest the mechanistic link between H. pylori infection combined with diabetes and increased generation of ROS and could play as an important image for high risk to atherosclerosis.

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“…Also, information on antibiotic resistance cannot be provided using this method [ 68 , 69 ]. As already mentioned, non-invasive methods such as urea breath test (UBT), stool antigen assay, and serology are frequently used [ 70 – 72 ]. These are widely performed on gastric juice, saliva, urine, and stool specimens.…”

Section: An Overview Of H Pylori Infectionmentioning

confidence: 99%

Helicobacter pylori: an up-to-date overview on the virulence and pathogenesis mechanisms

Sharndama

Elibe

2022

Braz J Microbiol

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Helicobacter pylori is an organism associated with ulcer disease and gastric cancer. The latter is one of the most prevalent malignancies and currently the fourth major cause of cancer-related deaths globally. The pathogen infects about 50% of the world population, and currently, no treatment ensures its total elimination. There has been an increase in our understanding of the pathophysiology and pathogenesis mechanisms of H. pylori over the years. H. pylori can induce several genetic alterations, express numerous virulence factors, and trigger diverse adaptive mechanisms during its adherence and colonization. For successful colonization and infection establishment, several effector proteins/toxins are released by the organism. Evidence is also available reporting spiral to coccoid transition as a unique tactic H. pylori uses to survive in the host’s gastrointestinal tract (GIT). Thus, the virulence and pathogenicity of H. pylori are under the control of complex interplay between the virulence factors, host, and environmental factors. Expounding the role of the various virulence factors in H. pylori pathogenesis and clinical outcomes is crucial for vaccine development and in providing and developing a more effective therapeutic intervention. Here we critically reflect on H. pylori infection and delineate what is currently known about the virulence and pathogenesis mechanisms of H. pylori .

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A novel one-step Helicobacter pylori saliva antigen test

Cited by 16 publications

References 20 publications

Review of current diagnostic methods and advances inHelicobacter pyloridiagnostics in the era of next generation sequencing

Review of current diagnostic methods and advances inHelicobacter pyloridiagnostics in the era of next generation sequencing

Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection

Helicobacter pylori: an up-to-date overview on the virulence and pathogenesis mechanisms

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