A Novel OmpR-Type Response Regulator Controls Multiple Stages of the Rhizobium etli – Phaseolus vulgaris N2-Fixing Symbiosis

Rodrı́guez, Susana; Correa‐Galeote, David; Sánchez-Pérez, Mishael; Ramı́rez, Mario; Isidra-Arellano, Mariel C; Reyero-Saavedra, María del Rocío; Zamorano‐Sánchez, David; Hernández, Georgina; Valdés‐López, Oswaldo; Girard, Lourdes

doi:10.3389/fmicb.2020.615775

Cited by 4 publications

(4 citation statements)

References 111 publications

(148 reference statements)

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“…In addition, a C4-dicarboxylate transport protein was found. The important role played by a new regulator from the OmpR family in the symbiosis of Rhizobium etli and Phaseolus vulgaris was recently reported (Rodríguez et al, 2020). Similarly, transcription regulators, related to biofilm formation, biosynthesis of antibiotics, response to osmotic stress and toxic chemicals, and pathogenicity were found enriched for bacteria colonizing plants (de Souza et al, 2019).…”

Section: Predictive Functional Signatures Of Plant Associated Micromo...mentioning

confidence: 91%

Deciphering Genomes: Genetic Signatures of Plant-Associated Micromonospora

et al. 2022

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Understanding plant-microbe interactions with the possibility to modulate the plant’s microbiome is essential to design new strategies for a more productive and sustainable agriculture and to maintain natural ecosystems. Therefore, a key question is how to design bacterial consortia that will yield the desired host phenotype. This work was designed to identify the potential genomic features involved in the interaction between Micromonospora and known host plants. Seventy-four Micromonospora genomes representing diverse environments were used to generate a database of all potentially plant-related genes using a novel bioinformatic pipeline that combined screening for microbial-plant related features and comparison with available plant host proteomes. The strains were recovered in three clusters, highly correlated with several environments: plant-associated, soil/rhizosphere, and marine/mangrove. Irrespective of their isolation source, most strains shared genes coding for commonly screened plant growth promotion features, while differences in plant colonization related traits were observed. When Arabidopsis thaliana plants were inoculated with representative Micromonospora strains selected from the three environments, significant differences were in found in the corresponding plant phenotypes. Our results indicate that the identified genomic signatures help select those strains with the highest probability to successfully colonize the plant and contribute to its wellbeing. These results also suggest that plant growth promotion markers alone are not good indicators for the selection of beneficial bacteria to improve crop production and the recovery of ecosystems.

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Section: Predictive Functional Signatures Of Plant Associated Micromo...mentioning

confidence: 91%

Deciphering Genomes: Genetic Signatures of Plant-Associated Micromonospora

et al. 2022

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“…In these, the canonical HK contains an input domain able to sense regulatory stimuli and subsequently the catalytic ATP binding (CA) domain phosphorylates a His residue in the dimeric histidine phosphotransfer (DHp) domain. The phosphoryl group is then transferred to an Asp residue in the receiver (REC) domain of a separate response regulator protein ( Gao and Stock, 2009 ; Rodríguez et al, 2020 ). In contrast, the predicted hybrid histidine kinase RHE_PE00024 is comprised of a putative N-terminal sensory region (containing two PAS sensor domains) and a kinase core containing both the CA and the DHp domains.…”

Section: Resultsmentioning

confidence: 99%

RdsA Is a Global Regulator That Controls Cell Shape and Division in Rhizobium etli

et al. 2022

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Unlike other bacteria, cell growth in rhizobiales is unipolar and asymmetric. The regulation of cell division, and its coordination with metabolic processes is an active field of research. In Rhizobium etli, gene RHE_PE00024, located in a secondary chromosome, is essential for growth. This gene encodes a predicted hybrid histidine kinase sensor protein, participating in a, as yet undescribed, two-component signaling system. In this work, we show that a conditional knockdown mutant (cKD24) in RHE_PE00024 (hereby referred as rdsA, after rhizobium division and shape) generates a striking phenotype, where nearly 64% of the cells present a round shape, with stochastic and uncoordinated cell division. For rod-shaped cells, a large fraction (12 to 29%, depending on their origin) present growth from the old pole, a sector that is normally inactive for growth in a wild-type cell. A fraction of the cells (1 to 3%) showed also multiple ectopic polar growths. Homodimerization of RdsA appears to be required for normal function. RNAseq analysis of mutant cKD24 reveals global changes, with downregulated genes in at least five biological processes: cell division, wall biogenesis, respiration, translation, and motility. These modifications may affect proper structuring of the divisome, as well as peptidoglycan synthesis. Together, these results indicate that the hybrid histidine kinase RdsA is an essential global regulator influencing cell division and cell shape in R. etli.

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“…The reaction mixture was incubated at 42 • C for 50 min, followed by heat inactivation at 70 • C for 15 min. The resulting first-strand cDNA was amplified using gene-specific primers provided by [60] (Table S1).…”

Section: Nodulation Genes Expression Analysismentioning

confidence: 99%

Reduction in the Use of Some Herbicides Favors Nitrogen Fixation Efficiency in Phaseolus vulgaris and Medicago sativa

Paniagua-López

Jiménez-Pelayo

Gómez-Fernández

et al. 2023

Plants

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In recent decades, the quality of agricultural soils has been seriously affected by the excessive application of pesticides, with herbicides being one of the most abundant. Continuous use of herbicides alters the soil microbial community and beneficial interactions between plants and bacteria such as legume-rhizobia spp. symbiosis, causing a decrease in the biological nitrogen fixation, which is essential for soil fertility. Therefore, the aim of this work was to study the effect of two commonly used herbicides (pendimethalin and clethodim) on the legume-rhizobia spp. symbiosis to improve the effectiveness of this process. Phaseolus vulgaris plants grown in pots with a mixture of soil:perlite (3:1 v/v), showed a 44% inhibition of nitrogen fixation rate with pendimethalin. However, clethodim, specifically used against monocots, did not induce significant differences. Additionally, we analyzed the effect of herbicides on root exudate composition, detecting alterations that might be interfering with the symbiosis establishment. In order to assess the effect of the herbicides at the early nodulation steps, nodulation kinetics in Medicago sativa plants inoculated with Sinorhizobium meliloti were performed. Clethodim caused a 30% reduction in nodulation while pendimethalin totally inhibited nodulation, producing a reduction in bacterial growth and motility as well. In conclusion, pendimethalin and clethodim application reduced the capacity of Phaseolus vulgaris and Medicago sativa to fix nitrogen by inhibiting root growth and modifying root exudate composition as well as bacterial fitness. Thus, a reduction in the use of these herbicides in these crops should be addressed to favor a state of natural fertilization of the soil through greater efficiency of leguminous crops.

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A Novel OmpR-Type Response Regulator Controls Multiple Stages of the Rhizobium etli – Phaseolus vulgaris N2-Fixing Symbiosis

Cited by 4 publications

References 111 publications

Deciphering Genomes: Genetic Signatures of Plant-Associated Micromonospora

Deciphering Genomes: Genetic Signatures of Plant-Associated Micromonospora

RdsA Is a Global Regulator That Controls Cell Shape and Division in Rhizobium etli

Reduction in the Use of Some Herbicides Favors Nitrogen Fixation Efficiency in Phaseolus vulgaris and Medicago sativa

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