A novel oleaginous yeast strain with high lipid productivity and its application to alternative biodiesel production

Areesirisuk, Atsadawut; Chiu, Chuen-Huey; Yen, Tsair‐Bor; Liu, C. H.; Guo, Jia

doi:10.1134/s0003683815030035

Cited by 19 publications

(13 citation statements)

References 33 publications

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“…Similar results were obtained by Papanikolaou and Aggelis [38] when using industrial glycerol, as well as Areesirisuk et al [39] when using hydrophilic carbon source. On the other hand, C. lipolytica showed the suitability molasses at 8% and increased the lipid production to 59.9%; however, molasses contains heavy metals [40].…”

Section: Discussionsupporting

confidence: 89%

Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

Souza

Rodríguez

Ribeaux

et al. 2016

IJMS

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Almost all oleaginous microorganisms are available for biodiesel production, and for the mechanism of oil accumulation, which is what makes a microbial approach economically competitive. This study investigated the potential that the yeast Candida lipolytica UCP0988, in an anamorphous state, has to produce simultaneously a bioemulsifier and to accumulate lipids using inexpensive and alternative substrates. Cultivation was carried out using waste soybean oil and corn steep liquor in accordance with 22 experimental designs with 1% inoculums (107 cells/mL). The bioemulsifier was produced in the cell-free metabolic liquid in the late exponential phase (96 h), at Assay 4 (corn steep liquor 5% and waste soybean oil 8%), with 6.704 UEA, IE24 of 96.66%, and showed an anionic profile. The emulsion formed consisted of compact small and stable droplets (size 0.2–5 µm), stable at all temperatures, at pH 2 and 4, and 2% salinity, and showed an ability to remove 93.74% of diesel oil from sand. The displacement oil (ODA) showed 45.34 cm2 of dispersion (central point of the factorial design). The biomass obtained from Assay 4 was able to accumulate lipids of 0.425 g/g biomass (corresponding to 42.5%), which consisted of Palmitic acid (28.4%), Stearic acid (7.7%), Oleic acid (42.8%), Linoleic acid (19.0%), and γ-Linolenic acid (2.1%). The results showed the ability of C. lipopytica to produce both bioemulsifier and biodiesel using the metabolic conversion of waste soybean oil and corn steep liquor, which are economic renewable sources.

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Section: Discussionsupporting

confidence: 89%

Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

Souza

Rodríguez

Ribeaux

et al. 2016

IJMS

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“…The lipids accumulated by oleaginous yeast are mainly composed of long-chain fatty acids, including oleic acid (C18:1), palmitic acid (C16:0), linoleic acid (C18:2) and stearic acid (C18:0), which are similar to the composition of plant oils and can be converted into biodiesel by enzymatic or inorganic catalysis (Ghanavati et al 2014 ; Spier et al 2015 ; Tanimura et al 2014a ; Wang and Ren 2014 ). Thus, much attention have been paid to various oleaginous yeasts for biodiesel production (Areesirisuk et al 2015 ; Deeba et al 2016 ; Poli et al 2014 ; Seo et al 2014 ; Sitepu et al 2014 ; Spier et al 2015 ; Tanimura et al 2014a , b ; Yang et al 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China

et al. 2017

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A total of ten marine yeast strains isolated from Bohai Sea, Northern China were identified to be members of three genera Rhodosporidium, Rhodotorula, and Cryptococcus. Two representative strains Rhodosporidium TJUWZ4 and Cryptococcus TJUWZA11 with high lipid content based on Nile red staining method were further characterized. A wide range of culture conditions (C and N sources, pH, temperature, salinity and C/N ratio) were tested to characterize the biomass and lipid production (yield and productivity) of these strains. Results indicated that Rhodosporidium TJUWZ4 was capable of achieving lipid yield up to 44% and 0.09 g/l–h productivity on glucose and peptone medium at pH 4, 20 °C, 30% salinity, and C/N 80. Three fatty acids, namely oleic acid (18:1), palmitic acid (C16:0) and linoleic acid (18:2) were the major intracellular fatty acids, which accounted for 90% of total lipids. With promising features for intracellular lipid accumulation, Rhodosporidium TJUWZ4 is a robust strain with great potentials for application in biodiesel production from renewable feedstocks.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0329-x) contains supplementary material, which is available to authorized users.

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“…In yeast, lipid bodies accumulate during stationary phase and can constitute up to the total lipid content of the cell (Zweytick et al 2000;Zhao et al 2008). Although there are limited literature that have reported on the intracellular lipid production in P. manshurica (Areesirisuk et al 2015), P. guilliermondii (Wang et al 2012;Ramírez-Castrillón et al 2017) and K. marxianus (Tampitak et al 2015), no solid references can be found on lipogenesis in C. parapsilosis, C. krusei and C. lusitaniae. Therefore, this study is the first to report lipid bodies accumulation in these three species.…”

Section: Resultsmentioning

confidence: 99%

“…There are growing interests in lipids or triacylglycerols (TAGs) from oleaginous yeasts in recent years (Areesirisuk et al 2015;Kahr et al 2015;Wang et al 2016;Ramírez-Castrillón et al 2017;Vincent et al 2018). This fungal group possesses the ability to convert agro and industrial residues into TAGs that are prime alternatives to lipids that are animal and/or plant-based (Pan et al 2009;Aghbashlo and Demirbas 2016).…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia

Vincent¹,

Hung²,

Baran³

et al. 2018

Biodiversitas

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Vincent M, Hung MC, Baran PRM, Azahari AS, Adeni DSA. 2018. Isolation, identification and diversity of oleaginous yeastsfrom Kuching, Sarawak, Malaysia. Biodiversitas 19: 1266-1272. The present study was performed to isolate, identify and determine thediversity of oleaginous yeasts from various sources in Kuching, Sarawak (Malaysia). Microscopic observations via light and scanningelectron microscope (SEM) indicated that the yeast isolates were in sizes ranging from 2-3 μm in width and 4-8 μm in length, typical ofmost unicellular ascomycotic fungi. Polymerase Chain Reaction (PCR) and molecular identification performed on the yeast isolates,targeting the D1/D2 region of the 26S rDNA, identified 6 yeast species from the 21 isolates, namely Pichia manshurica (5/21), Candidakrusei (8/21), Candida parapsilosis (1/21), Pichia guilliermondii (2/21), Clavispora lusitaniae (1/21) and Kluyveromyces marxianus(4/21). All 21 yeast isolates accumulated intracellular lipids when grown in nitrogen-limited medium, as tested via Sudan IV staining.The present study is the first to document the production of lipids bodies in C. krusei, C. parapsilosis, and C. lusitaniae. Furtherinvestigations to assess the growth kinetics, lipid production efficiencies and lipids profiles of these oleaginous yeasts may provideinsights into the possible utilization of these isolates for a variety of scientific, technical and industrial applications.

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A novel oleaginous yeast strain with high lipid productivity and its application to alternative biodiesel production

Cited by 19 publications

References 33 publications

Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

Waste Soybean Oil and Corn Steep Liquor as Economic Substrates for Bioemulsifier and Biodiesel Production by Candida lipolytica UCP 0998

Characterization and robust nature of newly isolated oleaginous marine yeast Rhodosporidium spp. from coastal water of Northern China

Isolation, identification and diversity of oleaginous yeasts from Kuching, Sarawak, Malaysia

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