A novel OFET-based biosensor for the selective and sensitive detection of lactate levels

Abstract

Biosensors based on organic field effect transistors (OFETs) are one of the more promising device applications in organic electronics. However, OFET-based biosensors are still in their early stages of development compared to other electrochemical biosensors. This study is the first to report on an extended-gate type organic field effect transistor (OFET) for lactate detection in aqueous media. Here, the extended-gate electrode of the OFET was modified with layers of a lactate oxidase and a horseradish peroxidase osmium-redox polymer on a flexible plastic film substrate for an enzymatic redox reaction of lactate. The device exhibited both high selectivity and sensitivity. The limit of detection (LOD) and the limit of quantification (LOQ) were estimated to be 66 nM and 220 nM, respectively, which are the sufficient detection limit for practical sensor applications. The obtained results confirm that extended-gate type OFET devices are applicable to enzyme-based biosensors for detecting lactate levels.

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

“…Even though the LOD value of our electrochemical lactate biosensor is clearly of the order of some electrochemical biosensors based on the use of screen-printed electrodes, as shown in Table 1, LOD values are higher than compared to nonelectrochemical approaches, see for example Minami et al (Minami et al 2015) who developed an organic field effect transistor. However, in their work, no real samples were explored limiting their work and sensor application.…”

Printed Organic Transistor‐Based Enzyme Sensor for Continuous Glucose Monitoring in Wearable Healthcare Applications

“…The extended‐gate type transistor is advantageous for stable and reproducible detection of analytes in a sample solution, because the sensing portion immersed in the solution can be completely isolated from the driving portion. We have developed the extended‐gate type organic transistor‐based biosensors using different types of bioreceptors: antibody, ionophore, and enzyme . Especially, enzyme‐based biosensors were found suitable for selective detection of small‐sized metabolites such as glucose and lactate in the bodily fluids.…”

“…We have developed the extendedgate type organic transistor-based biosensors using different types of bioreceptors: antibody, [16][17][18][19][20] ionophore, [21] and enzyme. [22][23][24] Especially, enzyme-based biosensors were found suitable for selective detection of small-sized metabolites such as glucose and lactate in the bodily fluids.…”

Estudo de transistores orgânicos por espectroscopia vibracional não linear e microscopia por modulação de carga

“…As ressonâncias nos espectros de SFG possuem três características notáveis: i-a posição do pico () O tensor susceptibilidade de 2ª ordem (2) ()  é de posto 3, com 27 elementos. Porém, em uma superfície isotrópica ao longo do plano xy, com z perpendicular à interface, esse número é reduzido para 7 termos:…”

“…Contudo, sob condições específicas, SHG e SFG permitem sondar a distribuição do campo elétrico e sua dinâmica dentro do semicondutor orgânico. 2549,50 Quando um forte campo elétrico DC (E0) está presente no volume de um material centrossimétrico, ele quebra a simetria de inversão e leva a um aumento significativo de (2) , que em geral pode ter duas contribuições. Uma é a orientação induzida por E0 dos dipolos no volume do material, que faz com que (2) ( 0 ) ≠ 0.…”