A Novel Nuclear Protein Phosphatase 2C Negatively Regulated by ABL1 is Involved in Abiotic Stress and Panicle Development in Rice

Li, Yusheng; Sun, Hui; Wang, Zhoufei; Duan, Min; Huang, Shengdong; Yang, Juan; Huang, Ji; Zhang, Hongsheng

doi:10.1007/s12033-012-9614-8

Cited by 32 publications

(23 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, phospholipase C and protein phosphatase 2C were showed to have a significant phosphoryltion changes under three stress conditions. Previous study also show that the phosphorylation levels of phospholipase C and protein phosphatase 2C is obviously affected (Li et al, 2013 ; Hwang et al, 2014 ; Wei et al, 2014 ; Zhang et al, 2014 ), which indicated that the signal pathway related to phospholipase C and protein phosphatase 2C played an important role in plants response to stress.…”

Section: Discussionmentioning

confidence: 80%

Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress

et al. 2015

View full text Add to dashboard Cite

Drought and heat stress, especially their combination, greatly affect crop production. Many studies have described transcriptome, proteome and phosphoproteome changes in response of plants to drought or heat stress. However, the study about the phosphoproteomic changes in response of crops to the combination stress is scare. To understand the mechanism of maize responses to the drought and heat combination stress, phosphoproteomic analysis was performed on maize leaves by using multiplex iTRAQ-based quantitative proteomic and LC-MS/MS methods. Five-leaf-stage maize was subjected to drought, heat or their combination, and the leaves were collected. Globally, heat, drought and the combined stress significantly changed the phosphorylation levels of 172, 149, and 144 phosphopeptides, respectively. These phosphopeptides corresponded to 282 proteins. Among them, 23 only responded to the combined stress and could not be predicted from their responses to single stressors; 30 and 75 only responded to drought and heat, respectively. Notably, 19 proteins were phosphorylated on different sites in response to the single and combination stresses. Of the seven significantly enriched phosphorylation motifs identified, two were common for all stresses, two were common for heat and the combined stress, and one was specific to the combined stress. The signaling pathways in which the phosphoproteins were involved clearly differed among the three stresses. Functional characterization of the phosphoproteins and the pathways identified here could lead to new targets for the enhancement of crop stress tolerance, which will be particularly important in the face of climate change and the increasing prevalence of abiotic stressors.

show abstract

Section: Discussionmentioning

confidence: 80%

Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Up regulation of RNA helicase in 7B-1 by ABA and mannitol could be associated with B-specific stress-tolerance of 7B-1 , however down regulation of this gene in D in no stress condition and by ABA and mannitol, suggests an alternative regulatory function for this gene rather than a stress-related one. PP2Cs act redundantly in ABA signaling and stress response in plants [ 50 – 53 ]. PP2Cs have been reported to be differentially expressed in response to different abiotic stresses [ 51 – 53 ].…”

Section: Discussionmentioning

confidence: 99%

“…PP2Cs act redundantly in ABA signaling and stress response in plants [ 50 – 53 ]. PP2Cs have been reported to be differentially expressed in response to different abiotic stresses [ 51 – 53 ]. Inactivation of PP2Cs by ABA receptors, such as OsPYL5 and AtPYL enhanced drought tolerance in rice and Arabidopsis , respectively [ 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

“…Inactivation of PP2Cs by ABA receptors, such as OsPYL5 and AtPYL enhanced drought tolerance in rice and Arabidopsis , respectively [ 51 , 52 ]. Expression of OsSIPP2C1 was up regulated in rice in response to ABA, drought and salinity [ 53 ]. Up regulation of PP2C in 7B-1 by ABA and mannitol in B could in part contribute to 7B-1 stress tolerance, but down regulation of this gene in WT and 7B-1 by mannitol in D, suggests a different mode of action for this gene in D as compared to B, which has yet to be understood.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

Omidvar

Fellner

2015

PLoS ONE

View full text Add to dashboard Cite

We reported earlier that 7B-1 mutant in tomato (Solanum lycopersicum L., cv. Rutgers), an ABA overproducer, is defective in blue light (B) signaling leading to B-specific resistance to abiotic and biotic stresses. Using a methylation-sensitive amplified polymorphism (MSAP) assay, a number of genes were identified, which were differentially methylated between 7B-1 and its wild type (WT) seedlings in white (W), blue (B), red (R) lights and dark (D) or in response to exogenous ABA and mannitol-induced stresses. The genomic methylation level was almost similar in different lights between 7B-1 and WT seedlings, while significant differences were observed in response to stresses in D, but not B. Using a cDNA-AFLP assay, several transcripts were identified, which were differentially regulated between 7B-1 and WT by B or D or in response to stresses. Blue light receptors cryptochrome 1 and 2 (CRY1 and CRY2) and phototropin 1 and 2 (PHOT1 and PHOT2) were not affected by the 7B-1 mutation at the transcriptional level, instead the mutation had likely affected downstream components of the light signaling pathway. 5-azacytidine (5-azaC) induced DNA hypomethylation, inhibited stem elongation and differentially regulated the expression of a number of genes in 7B-1. In addition, it was shown that mir167 and mir390 were tightly linked to auxin signaling pathway in 5-azaC-treated 7B-1 seedlings via the regulation of auxin-response factor (ARF) transcripts. Our data showed that DNA methylation remodeling is an active epigenetic response to different lights and stresses in 7B-1 and WT, and highlighted the differences in epigenetic and transcriptional regulation of light and stress responses between 7B-1 and WT. Furthermore, it shed lights on the crosstalk between DNA hypomethylation and miRNA regulation of ARFs expression. This information could also be used as a benchmark for future studies of male-sterility in other crops.

show abstract

“…Rice protoplast preparation and transformation were performed as previously described [22]. The subcellular distribution of the GFP fusion protein was examined by confocal laser scanning microscopy (Leica, TCS SP2).…”

Section: Methodsmentioning

confidence: 99%

OsSLI1, a Homeodomain Containing Transcription Activator, Involves Abscisic Acid Related Stress Response in Rice (Oryza sativaL.)

Huang

Duan

Liao

et al. 2014

The Scientific World Journal

Self Cite

View full text Add to dashboard Cite

Homeodomain-leucine zipper type I (HD-Zip I) proteins are involved in the regulation of plant development and response to environmental stresses. In this study, OsSLI1 (Oryza sativa stress largely induced 1), encoding a member of the HD-Zip I subfamily, was isolated from rice. The expression of OsSLI1 was dramatically induced by multiple abiotic stresses and exogenous abscisic acid (ABA). In silico sequence analysis discovered several cis-acting elements including multiple ABREs (ABA-responsive element binding factors) in the upstream promoter region of OsSLI1. The OsSLI1-GFP fusion protein was localized in the nucleus of rice protoplast cells and the transcriptional activity of OsSLI1 was confirmed by the yeast hybrid system. Further, it was found that OsSLI1 expression was enhanced in an ABI5-Like1 (ABL1) deficiency rice mutant abl1 under stress conditions, suggesting that ABL1 probably negatively regulates OsSLI1 gene expression. Moreover, it was found that OsSLI1 was regulated in panicle development. Taken together, OsSLI1 may be a transcriptional activator regulating stress-responsive gene expression and panicle development in rice.

show abstract

A Novel Nuclear Protein Phosphatase 2C Negatively Regulated by ABL1 is Involved in Abiotic Stress and Panicle Development in Rice

Cited by 32 publications

References 34 publications

Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress

Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress

DNA Methylation and Transcriptomic Changes in Response to Different Lights and Stresses in 7B-1 Male-Sterile Tomato

OsSLI1, a Homeodomain Containing Transcription Activator, Involves Abscisic Acid Related Stress Response in Rice (Oryza sativaL.)

Contact Info

Product

Resources

About