A Novel Neutrophil-Specific PET Imaging Agent: cFLFLFK-PEG-<sup>64</sup>Cu

Locke, Landon W.; Chordia, Mahendra D.; Zhang, Yi; Kundu, Bijoy; Kennedy, Dylan; Landseadel, Jessica; Xiao, Li; Fairchild, Karen D.; Berr, Stuart S.; Linden, Joel; Pan, Dongfeng

doi:10.2967/jnumed.108.056127

Cited by 96 publications

(120 citation statements)

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“…These improved probes have enhanced efficacy and have been used to detect severe neutrophil-associated lung inflammation and infection. [22][23][24] However, it is not clear whether a similar approach can be used to fabricate imaging probes for detecting and quantifying the degree of neutrophil recruitment related to medical device-associated foreign body reactions in vivo.…”

Section: Introductionmentioning

confidence: 99%

“…21 Recently, optical imaging systems have been developed to target neutrophils. Specifically, studies have shown that the peptide cinnamoyl-Phe-(D)Leu-Phe-(D)Leu-Phe (cFLFLF) has a high affinity to the formyl peptide receptor (FPR) of neutrophils, 22 and that near-infrared (NIR)-conjugated cFLFLF can be used to detect activated neutrophils in a mouse model of ear inflammation. 23 However, the high hydrophobicity of cFLFLF often leads to a relatively poor target-to-background ratio for in vivo imaging.…”

Section: Introductionmentioning

confidence: 99%

“…23 However, the high hydrophobicity of cFLFLF often leads to a relatively poor target-to-background ratio for in vivo imaging. 22 To address this deficiency, polyethylene glycol (PEG) polymers have been used to improve the hydrophilicity of the cFLFLF-NIR probes. These improved probes have enhanced efficacy and have been used to detect severe neutrophil-associated lung inflammation and infection.…”

Section: Introductionmentioning

confidence: 99%

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Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Zhou

Tsai

Hong

et al. 2012

IJN

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Neutrophils play an important role in implant-mediated inflammation and infection. Unfortunately, current methods which monitor neutrophil activity, including enzyme measurements and histological evaluation, require many animals and cannot be used to accurately depict the dynamic cellular responses. To understand the neutrophil interactions around implant-mediated inflammation and infection it is critical to develop methods which can monitor in vivo cellular activity in real time. In this study, formyl peptide receptor (FPR)-targeting nearinfrared nanoprobes were fabricated. This was accomplished by conjugating near-infrared dye with specific peptides having a high affinity to the FPRs present on activated neutrophils. The ability of FPR-targeting nanoprobes to detect and quantify activated neutrophils was assessed both in vitro and in vivo. As expected, FPR-targeting nanoprobes preferentially accumulated on activated neutrophils in vitro. Following transplantation, FPR-targeting nanoprobes preferentially accumulated at the biomaterial implantation site. Equally important, a strong relationship was observed between the extent of fluorescence intensity in vivo and the number of recruited neutrophils at the implantation site. Furthermore, FPR-targeting nanoprobes may be used to detect and quantify the number of neutrophils responding to a catheter-associated infection. The results show that FPR-targeting nanoprobes may serve as a powerful tool to monitor and measure the extent of neutrophil responses to biomaterial implants in vivo.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Real-time detection of implant-associated neutrophil responses using a formyl peptide receptor-targeting NIR nanoprobe

Zhou

Tsai

Hong

et al. 2012

IJN

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show abstract

“…In the current issue of The Journal of Nuclear Medicine, Locke et al (12) revisit the concept of in vivo leukocyte labeling, albeit with a somewhat different and provocative approach: using a positron, rather than a single-photon, emitter as the radiolabel. PET has important advantages over conventional g-camera imaging using single-photon emitters.…”

mentioning

confidence: 99%

“…Although Locke et al (12) and Liberatore et al (24) approach the radionuclide diagnosis of infection from rather different perspectives, their investigations should be viewed as complementary rather than competitive. None of the currently available radiotracers work equally well in all situations; labeled leukocytes perform better in certain circumstances, gallium in others, and 18 F-FDG in still others.…”

mentioning

confidence: 99%