A novel near-infrared fluorescent probe for the imaging of viscosity in cells and tumor-bearing mice

Abstract

A near-infrared fluorescence probe (IC-V) for detecting viscosity is constructed. The probe has a large stokes shift (170 nm) and an about 180-fold increase in fluorescence intensity at 700 nm....

Rational design of a lysosome-targeted fluorescent probe for monitoring the generation of hydroxyl radicals in ferroptosis pathways

“…All three molecules (HCy-Lyso, HCy-OH, and HCy-OH-H) were fully optimized with the density functional theory (DFT) and time-dependent density functional theory (TD-DFT) method using the B3LYP-D3 density functional, which is one of the most commonly used in the published literature for studying the structural properties of organic molecules. 48,49 B3LYP-D3 functional can also achieve a remarkable improvement in the accuracy of calculated results compared to the standard B3LYP functional. Besides, the 6-311G(d) basis set is carefully selected to obtain the best concordance with the experimental results.…”

Rational Design of Near-Infrared Fluorescent Probes for Accurately Tracking Lysosomal Viscosity with Allyl Snchoring Si-Rhodamine

“…What’s more, in a low-viscosity environment, free rotation of SR-Xs induces nonradiative relaxation of excited state energy, which leads to the decrease of fluorescence intensity. Along with environment viscosity gradually increasing, intramolecular rotation is suppressed, resulting in a significant enhancement in fluorescence intensity . Among them, SR-T-Al exhibits a more active fluorescence response to viscosity up to 10 folds ranging from 1.0 to 1410.4 cP.…”

Viscosity probes towards different organelles with red emission based on an identical hemicyanine structure

“…In addition, probes 1a–d exhibited the properties of red emission, which indicated that they had the advantages of low phototoxicity and small background interference and were suitable for high-fidelity bioimaging. 25,26 The optical properties of the probes and their biological properties were investigated in this paper.…”