A Novel Nanocomposite Scaffold Based on Polyurethane (PU) Containing Cobalt Nanoparticles (CoNPs) for Bone Tissue Engineering Applications

Abstract

Background: Bone tissue engineering, as a relatively new approach, has focused on combining biodegradable scaffolds, cells, and biologically active molecules for the recovery of different damaged tissues, such as bone defects. Polyurethane (PU), as a synthetic polymer, benefits from a porous structure which impersonates bone's natural environment. However, PU lacks osteoinduction activities. Cobalt nanoparticles (CoNPs) stimulate angiogenesis and biomineralization, which greatly favors osteogenesis. Methods: Here, we designed a novel scaffold based on PU and combined it with CoNPs for bone regeneration applications. The composition and structure of PU-CoNPs nanocomposite were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). MTT and AO data showed biocompatibility and enhanced viability and proliferation of fibroblasts on PU-CoNPs scaffold. Ascorbic acid-2-phosphate, β-glycerophosphate, and dexamethasone-induced osteogenesis for 14 days. objective: Induction of osteogenic differentiation in stem cells Results: The alkaline phosphatase test asserts the increased mineralization of hADSCs cultured on PU-CoNPs compared to pure PU scaffold. Further, the results disclosed an elevated osteogenic differentiation at the level of genes and proteins using immunocytochemical analysis (ICC) and quantitative real-time PCR (qPCR). method: The composition and structure of PU-CoNPs nanocomposite were characterized using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and differential scanning calorimetry (DSC). MTT and AO data showed biocompatibility and enhanced viability and proliferation of fibroblasts on PU-CoNPs scaffold. Ascorbic acid-2-phosphate, β-glycerophosphate, and dexamethasone-induced osteogenesis for 14 days. The alkaline phosphatase test asserts the increased mineralization of hADSCs cultured on PU-CoNPs compared to pure PU scaffold. Further, the results disclosed an elevated osteogenic differentiation at the level of genes and proteins using immunocytochemical analysis (ICC) and quantitative real-time PCR (qPCR). Conclusion: These findings provide an evidence that PU-CoNPs nanocomposite might be a promising candidate for bone repair applications. result: These findings provide evidence that PU-CoNPs nanocomposite might be a promising candidate for bone repair applications. other: -

Resorbable Biomaterials Used for 3D Scaffolds in Tissue Engineering: A Review

“…When applied in vivo, PCL proves to be stable and shows no visible degradation after 6 months [18]. PCL has been used in the production of hard (bone and dental applications) and soft tis- [16,[37][38][39][40][41] 2.1.1. Poly(ε-caprolactone) (PCL) PCL is an extremely tough aliphatic polyester and is widely used in various biomedical applications [16].…”