A novel NADPH-dependent aldehyde reductase gene from Saccharomyces cerevisiae NRRL Y-12632 involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion

Liu, Z. Lewis; Moon, Jon

doi:10.1016/j.gene.2009.06.018

Cited by 123 publications

(92 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, S. cerevisiae has the natural ability to reduce furfural and HMF to their corresponding less toxic alcohols by multiple dehydrogenases/reductases. 12,15,29 Researchers stated that, overexpression of the dehydrogenase/reductase genes (ari1, adh7, ald6 and adh6) increased enzyme activities for furfural and/or HMF reduction and simultaneously improved the tolerability of yeast against inhibitors. [12][13][14]30 Quantitative real-time PCR data confirmed the overexpression of tps1 and ari1 in engineered strain SCTADN.…”

Section: Discussionmentioning

confidence: 99%

“…Yeast strain with overexpression of aldehyde reductase gene showed not only more tolerant response to furfural and HMF, but earlier recoveries and a better growth as compare with wild type yeast strain. 12 All these aspects of the strain improvement played an important role in cell viability as well as ethanol production under several different stress conditions.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11][12] Many researches demonstrated that overexpression of the aldehyde dehydrogenase/reductase genes (ari1, ald6 and adh6) in S. cerevisiae not only could eliminate the toxicity of aldehydes but also improve tolerability. [12][13][14][15] Trehalose, a non-reducing disaccharide, works as an energy source in yeasts, bacteria, fungi, invertebrates, insects and plants. 16,17 Many research demonstrated that the higher trehalose accumulated in the yeast cell the higher tolerance against various environmental stresses, for instance ethanol stress, 18 heat stress, 19 saline stress, 20 and various other environmental stresses.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

et al. 2017

View full text Add to dashboard Cite

Lignocellulosic biomass is an attractive low-cost feedstock for bioethanol production. During bioethanol production, Saccharomyces cerevisiae, the common used starter, faces several environmental stresses such as aldehydes, glucose, ethanol, high temperature, acid, alkaline and osmotic pressure. The aim of this study was to construct a genetic recombinant S. cerevisiae starter with high tolerance against various environmental stresses. Trehalose-6-phosphate synthase gene (tps1) and aldehyde reductase gene (ari1) were co-overexpressed in nth1 (coded for neutral trehalase gene, trehalose degrading enzyme) deleted S. cerevisiae. The engineered strain exhibited ethanol tolerance up to 14% of ethanol, while the growth of wild strain was inhibited by 6% of ethanol. Compared with the wild strain, the engineered strain showed greater ethanol yield under high stress condition induced by combining 30% glucose, 30 mM furfural and 30 mM 5-hydroxymethylfurfural (HMF).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

et al. 2017

View full text Add to dashboard Cite

show abstract

“…In addition, a variety of inhibitory compounds are released, such as furfural, hydroxymethylfurfural, methylglyoxal, and acetate (4)(5)(6). It is therefore desirable not only to expand the substrate range of S. cerevisiae (3,(7)(8)(9) but also to increase its inhibitor tolerance (10)(11)(12)(13).…”

mentioning

confidence: 99%

Increasing Anaerobic Acetate Consumption and Ethanol Yields in Saccharomyces cerevisiae with NADPH-Specific Alcohol Dehydrogenase

Henningsen

Hon

Covalla

et al. 2015

Appl Environ Microbiol

View full text Add to dashboard Cite

Saccharomyces cerevisiae has recently been engineered to use acetate, a primary inhibitor in lignocellulosic hydrolysates, as a cosubstrate during anaerobic ethanolic fermentation. However, the original metabolic pathway devised to convert acetate to ethanol uses NADH-specific acetylating acetaldehyde dehydrogenase and alcohol dehydrogenase and quickly becomes constrained by limited NADH availability, even when glycerol formation is abolished. We present alcohol dehydrogenase as a novel target for anaerobic redox engineering of S. cerevisiae. Introduction of an NADPH-specific alcohol dehydrogenase (NADPH-ADH) not only reduces the NADH demand of the acetate-to-ethanol pathway but also allows the cell to effectively exchange NADPH for NADH during sugar fermentation. Unlike NADH, NADPH can be freely generated under anoxic conditions, via the oxidative pentose phosphate pathway. We show that an industrial bioethanol strain engineered with the original pathway (expressing acetylating acetaldehyde dehydrogenase from Bifidobacterium adolescentis and with deletions of glycerol-3-phosphate dehydrogenase genes GPD1 and GPD2) consumed 1.9 g liter ؊1 acetate during fermentation of 114 g liter ؊1 glucose. Combined with a decrease in glycerol production from 4.0 to 0.1 g liter ؊1 , this increased the ethanol yield by 4% over that for the wild type. We provide evidence that acetate consumption in this strain is indeed limited by NADH availability. By introducing an NADPH-ADH from Entamoeba histolytica and with overexpression of ACS2 and ZWF1, we increased acetate consumption to 5.3 g liter ؊1and raised the ethanol yield to 7% above the wild-type level. Saccharomyces cerevisiae is the principal microorganism used to produce ethanol, of which 93 billion liters were globally produced in 2014 (1). However, new strains are needed for the production of second-generation biofuels. Pretreatment and monomerization of lignocellulosic substrates produce complex sugar mixtures, including the C 5 sugars xylose and arabinose that are poorly fermented by most wild-type S. cerevisiae strains (2, 3). In addition, a variety of inhibitory compounds are released, such as furfural, hydroxymethylfurfural, methylglyoxal, and acetate (4-6). It is therefore desirable not only to expand the substrate range of S. cerevisiae (3, 7-9) but also to increase its inhibitor tolerance (10-13).Acetate is released during deacylation of hemicellulose and lignin and can be present in concentrations of Ͼ10 g liter Ϫ1 in cellulosic hydrolysates (4,14). At low pH particularly, this weak organic acid is a potent inhibitor of S. cerevisiae metabolism (15-17). Unfortunately, wild-type S. cerevisiae strains are poorly equipped to eliminate acetate from the medium under anoxic conditions. S. cerevisiae can grow on acetate as the sole carbon source under oxic conditions by converting acetate to acetyl coenzyme A (acetyl-CoA) with acetyl-CoA synthetase (ACS) (EC 6.2.1.1) and by either respiring acetyl-CoA in the tricarboxylic acid (TCA) cycle or upgrading acetyl-CoA to four-...

show abstract

“…As possíveis opções incluem a utilização de enzimas, como as lacases, ou suspensões celulares através da metabolização e/ou biotransformação destes compostos (LIU et al, 2004;MORENO et al, 2013). No entanto, é importante ressaltar, que não foi encontrada na literatura estudos de destoxificação biológica em hidrolisados hemicelulósicos de palha de arroz e de poda de oliveira, bem como a fermentação destes meios por Pichia stipitis, após processo de biotratamento.…”

Section: Métodos Biológicosunclassified

Avaliação das condições do tratamento biológico de hidrolisados hemicelulósicos visando melhorar a produção de etanol por<i> Scheffersomyces (Pichia) stipitis</i>

Fonseca¹

View full text Add to dashboard Cite

A novel NADPH-dependent aldehyde reductase gene from Saccharomyces cerevisiae NRRL Y-12632 involved in the detoxification of aldehyde inhibitors derived from lignocellulosic biomass conversion

Cited by 123 publications

References 40 publications

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

Metabolic engineering of Saccharomyces cerevisiae for improvement in stresses tolerance

Increasing Anaerobic Acetate Consumption and Ethanol Yields in Saccharomyces cerevisiae with NADPH-Specific Alcohol Dehydrogenase

Avaliação das condições do tratamento biológico de hidrolisados hemicelulósicos visando melhorar a produção de etanol por<i> Scheffersomyces (Pichia) stipitis</i>

Contact Info

Product

Resources

About