A Novel NAC Transcription Factor, IDEF2, That Recognizes the Iron Deficiency-responsive Element 2 Regulates the Genes Involved in Iron Homeostasis in Plants

Ogo, Yuko; Kobayashi, Takanori; Itai, Reiko Nakanishi; Nakanishi, Hiromi; Kakei, Yusuke; Takahashi, Michiko; Toki, Seiichi; Mori, Satoshi; Nishizawa, Naoko K.

doi:10.1074/jbc.m708732200

Cited by 196 publications

(181 citation statements)

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“…The identification of these transcription factors, as well as the ZDRE cis element they bind to and the target genes they regulate, constitutes an important step forward toward unraveling the regulation of the zinc homeostasis network in plants. Up to now, the best studied plant micronutrient homeostasis regulatory network is the iron deficiency response involving bHLH transcription factors such as the Arabidopsis FIT, bHLH038, bHLH039, bHLH100, and bHLH101 genes (22)(23)(24), for which no transcription factor binding site has been found yet, and the rice IDEF1 and IDEF2 proteinsbelonging to the ABI3/VP1 and NAC families of transcription factors-binding respectively to the IDE1 and IDE2 iron-deficiencyresponsive elements (25,26). The Arabidopsis bHLH transcription factors are strongly affected by iron status of the plant, whereas the rice transcription factors are not iron-regulated, more resembling the poor induction of bZIP19 and bZIP23 gene expression by zinc deficiency.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency

Assunção

Herrero

Lin

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

320

381

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Zinc is an essential micronutrient for all living organisms. When facing a shortage in zinc supply, plants adapt by enhancing the zinc uptake capacity. The molecular regulators controlling this adaptation are not known. We present the identification of two closely related members of the Arabidopsis thaliana basic-region leucinezipper (bZIP) transcription factor gene family, bZIP19 and bZIP23, that regulate the adaptation to low zinc supply. They were identified, in a yeast-one-hybrid screening, to associate to promoter regions of the zinc deficiency-induced ZIP4 gene of the Zrt-and Irtrelated protein (ZIP) family of metal transporters. Although mutation of only one of the bZIP genes hardly affects plants, we show that the bzip19 bzip23 double mutant is hypersensitive to zinc deficiency. Unlike the wild type, the bzip19 bzip23 mutant is unable to induce the expression of a small set of genes that constitutes the primary response to zinc deficiency, comprising additional ZIP metal transporter genes. This set of target genes is characterized by the presence of one or more copies of a 10-bp imperfect palindrome in their promoter region, to which both bZIP proteins can bind. The bZIP19 and bZIP23 transcription factors, their target genes, and the characteristic cis zinc deficiency response elements they can bind to are conserved in higher plants. These findings are a significant step forward to unravel the molecular mechanism of zinc homeostasis in plants, allowing the improvement of zinc bio-fortification to alleviate human nutrition problems and phytoremediation strategies to clean contaminated soils.biofortification | zinc homeostasis regulation | plant nutrition | abiotic stress | adaptation Z inc is an essential cofactor for many transcription factors, protein interaction domains, and enzymes in plants (1). Plants are thought to control zinc homeostasis by using a tightly regulated network of zinc status sensors and signal transducers controlling the coordinated expression of proteins involved in zinc acquisition from soil, mobilization between organs and tissues, and sequestration within cellular compartments (2). Although candidate genes for the required proteins such as zinc transporters and chelator biosynthesizing enzymes are found, no regulator of such network was ever identified in plants.Zinc influx facilitators, members of the ZIP family of metal transporters, are thought to play a major role in zinc uptake in plants (3). In Arabidopsis there are 15 ZIP genes (4), with ZIP1, ZIP2, ZIP3, and IRT3 functionally characterized as zinc uptake transporters (3, 5). Gene expression analysis has shown that approximately half of the ZIP genes are induced in response to zinc deficiency (3,(5)(6)(7)(8). The ZIP4 gene in particular is strongly induced upon shortage in zinc supply (3,(6)(7)(8).We focused on the promoter of this zinc-deficiency-responsive gene as the starting point for unraveling the regulation of the zinc homeostasis network in plants. By using DNA fragments of the zincdeficiency-responsive Arabidopsi...

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Section: Discussionmentioning

confidence: 99%

Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency

Assunção

Herrero

Lin

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

320

381

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“…Very recently, we successfully identified two rice transcription factors, IDE-binding factor 1 (IDEF1) and IDEF2, which specifically bind to IDE1 and IDE2, respectively [27,45]. IDEF1 and IDEF2 belong to uncharacterized branches of plant-specific transcription factor families ABI3/ VP1 and NAC, respectively, and exhibit novel properties of sequence recognition.…”

Section: Manipulating Transcription Factors Regulating the Fe Deficiementioning

confidence: 99%

Generation and Field Trials of Transgenic Rice Tolerant to Iron Deficiency

Kobayashi

Nakanishi

Takahashi

et al. 2008

Rice

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“…Low iron availability causes undesirable changes in plant growth and can be a major abiotic stress in crop production [5]. Thus, investigating the genes that respond to iron deficiency could lead to the production of plants with greater adaptive capacity in iron-deficient environments.…”

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confidence: 99%

“…Higher plants have evolved two iron uptake strategies for iron solubilization and acquisition [5]. Strategy I, the so-called reductive mechanism, functions in dicots and nongraminaceous monocots [6].…”

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Overexpression of OsDPR, a novel rice gene highly expressed under iron deficiency, suppresses plant growth

Naren

Zhang

et al. 2012

Sci. China Life Sci.

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Preliminary microarray analysis of cDNA from rice roots revealed an up-regulated transcript that was highly expressed in a five-day iron deficiency treatment. The entire sequence of this gene was determined by bioinformatics analysis. There were no proteins with significant levels of similarity detected in public databases. This novel gene with unknown biological function was designated as OsDPR (dwarf phenotype-related gene). We constructed a stable plant expression vector pCAM-BIA1302-OsDPR::GFP and produced transgenic tobacco plants. The phenotypes suggested that OsDPR restrained the growth of transformed plants. To understand the mechanisms of this suppression effect, cell size and number were compared between transformants and wild-type plants. The cell proliferation rate was lower in OsDPR transgenic BY-2 cells than in wild-type cells, but OsDPR expression did not affect cell size. Moreover, the cell division-related gene CyclinD2.1, which is involved in plant growth, was down-regulated in transgenic tobacco plants. These findings suggested that the novel iron-regulated gene OsDPR is responsible for the nanism phenotype of transgenic seedlings because of the inhibition of plant cell proliferation.OsDPR, iron deficiency, restrained growth, cell division, CyclinD2.1 Citation:Naren, Zhang P, Ma D K, et al. Overexpression of OsDPR, a novel rice gene highly expressed under iron deficiency, suppresses plant growth. Sci

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A Novel NAC Transcription Factor, IDEF2, That Recognizes the Iron Deficiency-responsive Element 2 Regulates the Genes Involved in Iron Homeostasis in Plants

Cited by 196 publications

References 45 publications

Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency

Arabidopsis thaliana transcription factors bZIP19 and bZIP23 regulate the adaptation to zinc deficiency

Generation and Field Trials of Transgenic Rice Tolerant to Iron Deficiency

Overexpression of OsDPR, a novel rice gene highly expressed under iron deficiency, suppresses plant growth

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