A novel NAC family transcription factor <i>SPR</i> suppresses seed storage protein synthesis in wheat

Shen, Lisha; Luo, Guangbin; Song, Yuan‐Zong; Xu, Junyang; Ji, Jingjing; Zhang, Chi; Gregová, E.; Yang, Wenlong; Li, Xin; Sun, Jiazhu; Zhan, Kai; Cui, Dangqun; Liu, Dongcheng

doi:10.1111/pbi.13524

Cited by 25 publications

(24 citation statements)

References 108 publications

(161 reference statements)

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“…Considering the large gene number and high nucleotide sequence identities (>90%) among members of each SSP type, only the expressed genes that could represent most of the sequence variations in the phylogenetic analyses according to Shen et al . ( 2020 ) were selected in both in vivo and in vitro assays in this work. The DNA sequences of all genes in this work can be found in GenBank and in the genome sequence data of T. urartu and T. aestivum under the following accession numbers: TuODORANT1 (GenBank accession No.…”

Section: Methodsmentioning

confidence: 99%

The MYB family transcription factor TuODORANT1 from Triticum urartu and the homolog TaODORANT1 from Triticum aestivum inhibit seed storage protein synthesis in wheat

Luo

Shen

Song

et al. 2021

Plant Biotechnology Journal

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Summary Seed storage proteins (SSPs) are determinants of wheat end‐product quality. SSP synthesis is mainly regulated at the transcriptional level. Few transcriptional regulators of SSP synthesis have been identified in wheat and this study aims to identify novel SSP gene regulators. Here, the R2R3 MYB transcription factor TuODORANT1 from Triticum urartu was found to be preferentially expressed in the developing endosperm during grain filling. In common wheat (Triticum aestivum) overexpressing TuODORANT1, the transcription levels of all the SSP genes tested by RNA‐Seq analysis were reduced by 49.71% throughout grain filling, which contributed to 13.38%–35.60% declines in the total SSP levels of mature grains. In in vitro assays, TuODORANT1 inhibited both the promoter activities and the transcription of SSP genes by 1‐ to 13‐fold. The electrophoretic mobility shift assay (EMSA) and ChIP‐qPCR analysis demonstrated that TuODORANT1 bound to the cis‐elements 5′‐T/CAACCA‐3′ and 5′‐T/CAACT/AG‐3′ in SSP gene promoters both in vitro and in vivo. Similarly, the homolog TaODORANT1 in common wheat hindered both the promoter activities and the transcription of SSP genes by 1‐ to 112‐fold in vitro. Knockdown of TaODORANT1 in common wheat led to 14.73%–232.78% increases in the transcription of the tested SSP genes, which contributed to 11.43%–19.35% elevation in the total SSP levels. Our data show that both TuODORANT1 and TaODORANT1 are repressors of SSP synthesis.

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Section: Methodsmentioning

confidence: 99%

The MYB family transcription factor TuODORANT1 from Triticum urartu and the homolog TaODORANT1 from Triticum aestivum inhibit seed storage protein synthesis in wheat

Luo

Shen

Song

et al. 2021

Plant Biotechnology Journal

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“…It is well known that NAM-ATAF-CUC (NAC) TFs participate in a series of biological processes, including abiotic and biotic stress responses and organ development ( Uauy et al, 2006 ; Xue et al, 2011 ; Liang et al, 2014 ; Borrill et al, 2017 ; Guerin et al, 2019 ). Recently, some NAC TFs (TaNAC019, TaNAC100, and TaSPR) in wheat have been identified to regulate grain storage protein synthesis ( Gao et al, 2021 ; Li et al, 2021b ; Shen et al, 2021 ). TaNAC019, a wheat endosperm-specific NAC TF, binds to the motif ([AT]NNNNNN[ATC][CG]A[CA]GN[ACT]A) in the promoter region of Glu-1 genes.…”

Section: Gene Network Regulating Storage Protein Biosynthesismentioning

confidence: 99%

“…TaNAC019-BI can improve flour processing quality and is an important candidate gene for wheat quality improvement ( Gao et al, 2021 ). However, two recent studies demonstrated that both TaNAC100 and TaSPR function as repressors of seed storage protein expression in common wheat, indicating that further research is needed for better utilization of such TFs in breeding ( Li et al, 2021b ; Shen et al, 2021 ). The TaDME (wheat DEMETER) gene encoding 5-methylcytosine DNA glycosylase on the long arm of group 5 chromosomes suppresses the LMW-GS and gliadin gene expression by activating the demethylation of their promoters in the endosperm ( Wen et al, 2012 ).…”

Section: Gene Network Regulating Storage Protein Biosynthesismentioning

confidence: 99%

Wheat Quality Formation and Its Regulatory Mechanism

Peng

Zhao

et al. 2022

Front. Plant Sci.

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Elucidation of the composition, functional characteristics, and formation mechanism of wheat quality is critical for the sustainable development of wheat industry. It is well documented that wheat processing quality is largely determined by its seed storage proteins including glutenins and gliadins, which confer wheat dough with unique rheological properties, making it possible to produce a series of foods for human consumption. The proportion of different gluten components has become an important target for wheat quality improvement. In many cases, the processing quality of wheat is closely associated with the nutritional value and healthy effect of the end-products. The components of wheat seed storage proteins can greatly influence wheat quality and some can even cause intestinal inflammatory diseases or allergy in humans. Genetic and environmental factors have great impacts on seed storage protein synthesis and accumulation, and fertilization and irrigation strategies also greatly affect the seed storage protein content and composition, which together determine the final end-use quality of wheat. This review summarizes the recent progress in research on the composition, function, biosynthesis, and regulatory mechanism of wheat storage proteins and their impacts on wheat end-product quality.

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“…as NAC-binding motifs using ChIP-seq and RNA-seq in soybean (Glycine max). In wheat, CANNTG (E-box) and ACGCAG serve as NAC-binding motifs (Liu et al, 2020;Shen et al, 2020). We queried the motifs in different promoter regions of TaGBBS1 and TaSUS2 using PLACE (https://www.dna.affrc.…”

Section: Shamimuzzaman and Vodkinthree (2013) Defined G [A/t] G [A/g]...mentioning

confidence: 99%

TaNAC100 acts as an integrator of seed protein and starch synthesis exerting pleiotropic effects on agronomic traits in wheat

Xie

Tian

et al. 2021

The Plant Journal

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High-molecular-weight glutenin subunits (HMW-GS) are major components of seed storage proteins (SSPs) and largely determine the processing properties of wheat (Triticum aestivum) flour. HMW-GS are encoded by the GLU-1 loci and regulated at the transcriptional level by interaction between cis-elements and transcription factors (TFs). We recently validated the function of conserved cis-regulatory modules (CCRMs) in GLU-1 promoters, but their interacting TFs remained uncharacterized. Here we identified a CCRM-binding NAM-ATAF-CUC (NAC) protein, TaNAC100, through yeast one-hybrid (Y1H) library screening. Transactivation assays demonstrated that TaNAC100 could bind to the GLU-1 promoters and repress their transcription activity in tobacco (Nicotiana benthamiana). Overexpression of TaNAC100 in wheat significantly reduced the contents of HMW-GS and other SSPs as well as total seed protein. This was confirmed by transcriptome analyses. Conversely, enhanced expression of TaNAC100 increased seed starch contents and expression of key starch synthesis-related genes, such as TaGBSS1 and TaSUS2. Y1H assays also indicated TaNAC100 binding with the promoters of TaGBSS1 and TaSUS2. These results suggest that TaNAC100 functions as a hub controlling seed protein and starch synthesis. Phenotypic analyses showed that TaNAC100 overexpression repressed plant height, increased heading date, and promoted seed size and thousand kernel weight. We also investigated sequence variations in a panel of cultivars, but did not identify significant association of TaNAC100 haplotypes with agronomic traits. The findings not only uncover a useful gene for wheat breeding but also provide an entry point to reveal the mechanism underlying metabolic balance of seed storage products.

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A novel NAC family transcription factor SPR suppresses seed storage protein synthesis in wheat

Cited by 25 publications

References 108 publications

The MYB family transcription factor TuODORANT1 from Triticum urartu and the homolog TaODORANT1 from Triticum aestivum inhibit seed storage protein synthesis in wheat

The MYB family transcription factor TuODORANT1 from Triticum urartu and the homolog TaODORANT1 from Triticum aestivum inhibit seed storage protein synthesis in wheat

Wheat Quality Formation and Its Regulatory Mechanism

TaNAC100 acts as an integrator of seed protein and starch synthesis exerting pleiotropic effects on agronomic traits in wheat

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