A novel mutation from gene splicing of a voltage-gated sodium channel in a marine copepod and its potential effect on channel function

Chen, Lihua; Zhang, Huan; Finiguerra, Michael; Bobkov, Yuriy V.; Bouchard, Christelle; Avery, David E.; Anderson, Peter; Lin, Senjie; Dam, Hans G.

doi:10.1016/j.jembe.2015.04.003

Cited by 10 publications

(6 citation statements)

References 49 publications

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“…Phylogenetic analysis of voltage‐gated sodium channels Na v 1 in L. salmonis . The tree was constructed based on the amino acid sequences of domain I of Na v 1 paralogues identified in a salmon louse transcriptome subjected to a maximum likelihood analysis using RAxML . For phylogenetic reconstruction, the WAG substitution model of rate heterogeneity among sites were implemented.…”

Section: Resultsmentioning

confidence: 83%

“…To identify L. salmonis Na v homologues, BLASTn searches were performed on the L. salmonis salmonis genome assembly (ftp://ftp.ensemblgenomes.org/pub/metazoa/release-36/fasta/lepeophtheirus_salmonis) using the Na v 1 cDNA sequence of the marine copepod Acartia hudsonica Pinhey, 1926 [GenBank: KP985762] as the query . Three hits of high homology scores (E‐value <10 −4 ) were obtained, suggesting that three loci encoding Na v homologues exist in L. salmonis (Supporting information, Table S1).…”

Section: Methodsmentioning

confidence: 99%

“…Phylogenetic analyses of L. salmonis Na v protein sequences took into account sodium channels from a number of arthropods, including several crustaceans and the planktonic copepod A. hudsonica . Na v channels are formed by four homologous domains (I‐IV) responsible for channel opening, ion selectivity and voltage sensing linked by highly variable intracellular loops (Fig.…”

Section: Methodsmentioning

confidence: 99%

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Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)

Carmona‐Antoñanzas

Helgesen

Humble

et al. 2018

Pest Management Science

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Section: Resultsmentioning

confidence: 83%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)

Carmona‐Antoñanzas

Helgesen

Humble

et al. 2018

Pest Management Science

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“…For example, the fitness of Acartia hudsonica increased for several generations after feeding on A. catenella (reported as A. fundyense), which indicates that adaptation occurred (Finiguerra et al, 2014). Mutations of the sodium channels have been suggested as a possible mechanism (Avery and Dam, 2007;Chen et al, 2015). However, exposure of A. hudsonica to HA did not induce an increased proportion of mutant isoforms (Finiguerra et al, 2014), which suggests that mutation in the sodium channel was not responsible.…”

Section: Introductionmentioning

confidence: 99%

The Influence of the Toxin-Producing Dinoflagellate, Alexandrium catenella (1119/27), on the Survival and Reproduction of the Marine Copepod, Acartia tonsa, During Prolonged Exposure

et al. 2021

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Copepods can feed on, and may regulate, the blooms of harmful algae (HA), and may also facilitate dinoflagellate blooms by inducing toxin production and through selective grazing. However, exposure to HA may also cause mortality and reproductive impairment in copepods, with detrimental effects at the population-scale. Here we present the toxin profile of the dinoflagellate, Alexandrium catenella (formerly Alexandrium tamarense), and examine how it affects the survival and reproduction of the cosmopolitan marine copepod, Acartia tonsa. Healthy adult copepods were exposed to mono-specific diets of toxic and non-toxic strains of A. catenella (1119/27 and 1119/19, respectively) and non-toxic Rhodomonas sp. for 10 days alongside unfed controls to examine how their survival was influenced by likely HA bloom conditions. Additional 2-day experiments examined how their egg production rate and hatching success were affected by food deprivation, toxic A. catenella, a non-toxic alternative and a mixture of toxic and non-toxic prey, at high and low concentrations. Survival of A. tonsa declined over the 10-day experiment in all treatments but was not significantly lower in the toxic A. catenella treatment; mortality was only significantly enhanced in the unfed animals, which showed 100% mortality after 9 days. Egg production rates and hatching success from females in the unfed and toxic A. catenella treatments were all significantly lower than values observed in females fed Rhodomonas sp. or non-toxic A. catenella. Animals offered 1,000 μg C L–1 of Rhodomonas sp. and a 50:50 mixture of toxic A. catenella and Rhodomonas sp. produced significantly more eggs than animals fed toxic A. catenella alone. These results were not apparent at prey concentrations of 100 μg C L–1. The percentages of eggs to successfully hatch from females offered mono-specific diets of toxic A. catenella were always close to zero. Collectively, our results indicate that adult female A. tonsa can acquire sufficient energy from toxic A. catenella to survive, but suffer reproductive impairment when feeding on this prey alone.

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“…Understanding individual as well as population performance of copepods at the molecular level is the key to gaining insights into the mechanisms regulating their growth and development, physiological functions, and response to the environmental changes 10 . In recent years, an increasing number of transcriptomic studies have been conducted with copepods at different growth stages, fed with different algae, and exposed to different stressors.…”

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confidence: 99%

Spliced leader RNA trans-splicing discovered in copepods

Yang

Zhuang

et al. 2015

Sci Rep

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Copepods are one of the most abundant metazoans in the marine ecosystem, constituting a critical link in aquatic food webs and contributing significantly to the global carbon budget, yet molecular mechanisms of their gene expression are not well understood. Here we report the detection of spliced leader (SL) trans-splicing in calanoid copepods. We have examined nine species of wild-caught copepods from Jiaozhou Bay, China that represent the major families of the calanoids. All these species contained a common 46-nt SL (CopepodSL). We further determined the size of CopepodSL precursor RNA (slRNA; 108-158 nt) through genomic analysis and 3′-RACE technique, which was confirmed by RNA blot analysis. Structure modeling showed that the copepod slRNA folded into typical slRNA secondary structures. Using a CopepodSL-based primer set, we selectively enriched and sequenced copepod full-length cDNAs, which led to the characterization of copepod transcripts and the cataloging of the complete set of 79 eukaryotic cytoplasmic ribosomal proteins (cRPs) for a single copepod species. We uncovered the SL trans-splicing in copepod natural populations, and demonstrated that CopepodSL was a sensitive and specific tool for copepod transcriptomic studies at both the individual and population levels and that it would be useful for metatranscriptomic analysis of copepods.

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A novel mutation from gene splicing of a voltage-gated sodium channel in a marine copepod and its potential effect on channel function

Cited by 10 publications

References 49 publications

Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)

Mutations in voltage‐gated sodium channels from pyrethroid resistant salmon lice (Lepeophtheirus salmonis)

The Influence of the Toxin-Producing Dinoflagellate, Alexandrium catenella (1119/27), on the Survival and Reproduction of the Marine Copepod, Acartia tonsa, During Prolonged Exposure

Spliced leader RNA trans-splicing discovered in copepods

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