A novel multifunctional bilayer scaffold based on chitosan nanofiber/alginate-gelatin methacrylate hydrogel for full-thickness wound healing

Asadi, Nahideh; Mehdipour, Ahmad; Ghorbani, Marjan; Mesgari‐Abbasi, Mehran; Akbarzadeh, Abolfazl; Davaran, Soodabeh

doi:10.1016/j.ijbiomac.2021.10.180

Cited by 40 publications

(25 citation statements)

References 79 publications

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“…On the other hand, the biocompatibility of these polymeric materials has not been tested since, as previously mentioned, it has been widely proven by other authors in both in vitro and in vivo experiments for different tissues, − including the bone and the cartilage. − …”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, different approaches have been tested to develop new biomaterials as cartilage tissue replacement, ,− in particular, and tissue engineering, in general. − Polymers and polymeric composites are the most commonly used materials considered for this application − due to their biocompatibility and tunable properties, − which enable development of their chemical structure or composition according to the final aim. In this sense, among naturally occurring polymers, gelatin and alginate stand out for the possibility of tuning their mechanical properties thanks to the degree of cross-linking. , Their biocompatibility have been widely investigated in different in vitro and in vivo experiments for diverse tissues, − including the bone and the cartilage. − In addition, they form hydrogels that allow the easy preparation of composites, − for example, bioactive glasses, , for the improvement of their osseointegration capacity. , …”

Section: Introductionmentioning

confidence: 99%

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Fabrication and Characterization of Bioactive Gelatin–Alginate–Bioactive Glass Composite Coatings on Porous Titanium Substrates

Begines

Arévalo

Romero

et al. 2022

ACS Appl. Mater. Interfaces

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In this research work, the fabrication of biphasic composite implants has been investigated. Porous, commercially available pure Ti (50 vol % porosity and pore distributions of 100−200, 250−355, and 355−500 μm) has been used as a cortical bone replacement, while different composites based on a polymer blend (gelatin and alginate) and bioactive glass (BG) 45S5 have been applied as a soft layer for cartilage tissues. The microstructure, degradation rates, biofunctionality, and wear behavior of the different composites were analyzed to find the best possible coating. Experiments demonstrated the best micromechanical balance for the substrate containing 200−355 μm size range distribution. In addition, although the coating prepared from alginate presented a lower mass loss, the composite containing 50% alginate and 50% gelatin showed a higher elastic recovery, which entails that this type of coating could replicate the functions of the soft tissue in areas of the joints. Therefore, results revealed that the combinations of porous commercially pure Ti and composites prepared from alginate/gelatin/45S5 BG are candidates for the fabrication of biphasic implants not only for the treatment of osteochondral defects but also potentially for any other diseases affecting simultaneously hard and soft tissues.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fabrication and Characterization of Bioactive Gelatin–Alginate–Bioactive Glass Composite Coatings on Porous Titanium Substrates

Begines

Arévalo

Romero

et al. 2022

ACS Appl. Mater. Interfaces

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show abstract

“…This result could be attributed to the flat, cell-adhesion conductive medium, which facilitates simple and efficient cell attachment, cell signaling, and cell proliferation. 45 Furthermore, studies have shown that at high cell densities, the pore spaces in the scaffolds fill rapidly, resulting in reduced proliferation because cell contact inhibition growth. 45 CCK8 results demonstrated that nanofibers can help maintain cells and provide the appropriate growth environment for fibroblasts.…”

Section: Resultsmentioning

confidence: 99%

“… 45 Furthermore, studies have shown that at high cell densities, the pore spaces in the scaffolds fill rapidly, resulting in reduced proliferation because cell contact inhibition growth. 45 CCK8 results demonstrated that nanofibers can help maintain cells and provide the appropriate growth environment for fibroblasts. In the process of wound healing, fibroblast migration from various sources contributes to the formation of granulation tissue and extracellular matrix, which is very important for wound healing.…”

Section: Resultsmentioning

confidence: 99%

In vitro and in vivo Evaluation of the Bioactive Nanofibers-Encapsulated Benzalkonium Bromide for Accelerating Wound Repair with MRSA Skin Infection

Ran¹,

Peng²,

Wang³

et al. 2022

IJN

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Purpose Developing the ideal drug or dressing is a serious challenge to controlling the occurrence of antibacterial infection during wound healing. Thus, it is important to prepare novel nanofibers for a wound dressing that can control bacterial infections. In our study, the novel self-assembled nanofibers of benzalkonium bromide with bioactive peptide materials of IKVAV and RGD were designed and fabricated. Methods Different drug concentration effects of encapsulation efficacy, swelling ratio and strength were determined. Its release profile in simulated wound fluid and its cytotoxicity were studied in vitro. Importantly, the antibacterial efficacy, inhibition of biofilm formation effect and wound healing against MRSA infections in vitro and in vivo were performed after observing the tissue toxicity in vivo. Results It was found that the optimized drug load (0.8%) was affected by the encapsulation efficacy, swelling ratio, and strength. In addition, the novel nanofibers with average diameter (222.0 nm) and stabile zeta potential (−11.2 mV) have good morphology and characteristics. It has a delayed released profile in the simulated wound fluid and good biocompatibility with L929 cells and most tissues. Importantly, the nanofibers were shown to improve antibacterial efficacy, inhibit biofilm formation, and lead to accelerated wound healing following infection with methicillin-resistant Staphylococcus aureus . Conclusion These data suggest that novel nanofibers could effectively shorten the wound-healing time by inhibiting biofilm formation, which make it promising candidates for treatment of MRSA-induced wound infections.

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“…Exceptional properties, such as biocompatibility, the type of biodegradation, and good mechanical and physicochemical parameters [ 7 , 8 ] have stimulated extensive research into the potential applications of PCL in the biomedical field. For decades, PCL was widely used as a component of medical sutures [ 9 ], wound dressings [ 10 , 11 ], contraceptive devices [ 12 ], fixation devices [ 13 ], and filling materials in dentistry [ 14 , 15 ]. Recently, thanks to the development of tissue engineering, attention has again been drawn to PCL.…”

Section: Introductionmentioning

confidence: 99%

Modified Histopathological Protocol for Poly-ɛ-Caprolactone Scaffolds Preserving Their Trabecular, Honeycomb-like Structure

et al. 2022

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Poly-ɛ-caprolactone (PCL) is now widely studied in relation to the engineering of bone, cartilage, tendons, and other tissues. Standard histological protocols can destroy the carefully created trabecular and honeycomb-like architecture of PCL scaffolds, and could lead to scaffold fibers swelling, resulting in the displacement or compression of tissues inside the scaffold. The aim of this study was to modify a standard histopathological protocol for PCL scaffold preparation and evaluate it on porous cylindrical PCL scaffolds in a rat model. In 16 inbred Wag rats, 2 PCL scaffolds were implanted subcutaneously to both inguinal areas. Two months after implantation, harvested scaffolds were first subjected to μCT imaging, and then to histopathological analysis with standard (left inguinal area) and modified histopathological protocols (right inguinal area). To standardize the results, soft tissue percentages (STPs) were calculated on scaffold cross-sections obtained from both histopathological protocols and compared with corresponding µCT cross-sections. The modified protocol enabled the assessment of almost 10× more soft tissues on the scaffold cross-section than the standard procedure. Moreover, STP was only 1.5% lower than in the corresponding µCT cross-sections assessed before the histopathological procedure. The presented modification of the histopathological protocol is cheap, reproducible, and allows for a comprehensive evaluation of PCL scaffolds while maintaining their trabecular, honeycomb-like structure on cross-sections.

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A novel multifunctional bilayer scaffold based on chitosan nanofiber/alginate-gelatin methacrylate hydrogel for full-thickness wound healing

Cited by 40 publications

References 79 publications

Fabrication and Characterization of Bioactive Gelatin–Alginate–Bioactive Glass Composite Coatings on Porous Titanium Substrates

Fabrication and Characterization of Bioactive Gelatin–Alginate–Bioactive Glass Composite Coatings on Porous Titanium Substrates

In vitro and in vivo Evaluation of the Bioactive Nanofibers-Encapsulated Benzalkonium Bromide for Accelerating Wound Repair with MRSA Skin Infection

Modified Histopathological Protocol for Poly-ɛ-Caprolactone Scaffolds Preserving Their Trabecular, Honeycomb-like Structure

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