A novel model for equine recurrent airway obstruction

Bowles, Kimberly S.; Beadle, R. E.; Mouch, Saif Abu; Pourciau, Susan; Littlefield-Chabaud, Martha A.; Blanc, Ch.; Mistric, L; Fermaglich, Daniel H; Horohov, David W.

doi:10.1016/s0165-2427(02)00081-8

Cited by 28 publications

(15 citation statements)

References 23 publications

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“…Furthermore, the initial tracheal concentrations of IL‐17 varied greatly among horses. This fits to the reported local variations of cytokines in asthma‐afflicted horses probably due to genetic factors, the disease stage, and varying environmental allergen exposures 35, 36, 37, 38, 39, 62.…”

Section: Discussionsupporting

confidence: 89%

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

Klier

Geis

Steuer

et al. 2017

Immunity Inflam & Disease

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IntroductionNew therapeutic strategies to modulate the immune response of human and equine allergic asthma are still under extensive investigation. Immunomodulating agents stimulating T‐regulatory cells offer new treatment options beyond conventional symptomatic treatment or specific immunotherapy for human and equine allergic airway diseases, with the goal of a homoeostatic T‐helper cell balance. The aim of this study was to evaluate the effects of a nebulized gelatin nanoparticle‐CpG formulation (CpG‐GNP) with and without specific allergens for the treatment of spontaneous allergic equine asthma as a model for human asthma.MethodsTwenty equine asthma‐affected horses were treated either with CpG‐GNP alone or CpG‐GNP with allergens. Two specific allergens were selected for each horse based on history and an in‐vitro test. Each horse received seven administrations of the respective nebulized composition and was examined before treatment, immediately after and 6 weeks after the treatment course.ResultsClinical parameters such as breathing rate, indirect interpleural measurement, arterial blood gases, amount of tracheal mucus and percentage of neutrophils and cytokines in tracheal washes and serum samples were evaluated. Treatment with CpG‐GNP alone as well as in combinations with relevant allergens resulted in clinical improvement of nasal discharge, breathing rate, amount of secretion and viscosity, neutrophil percentage and partial oxygen pressure directly after and 6 weeks after treatment. There were no significant differences between the two treatments in clinical parameters or local cytokine profiles in the tracheal wash fluid (IL‐10, IFN‐g, and IL‐17). IL‐4 concentrations decreased significantly in both groups.ConclusionNonspecific CpG‐GNP‐based immunotherapy shows potential as a treatment for equine and possibly also human allergic asthma.

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Section: Discussionsupporting

confidence: 89%

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

Klier

Geis

Steuer

et al. 2017

Immunity Inflam & Disease

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show abstract

“…Moreover, as regards the number of cytokines analyzed, numerous studies have focused on only a single cytokine (Franchini et al 2000, Debrue et al 2005, two cytokines (Laan et al 2005) or three cytokines (Bowles et al 2002, Cordeau et al 2004) while other Authors have investigated more extensive profiles of different immunomodulatory proteins (Giguère et al 2002, Ainsworth et al 2003, Kleiber et al 2005, Ainsworth et al 2007, Riihimäki et al 2008a, 2008b.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, some Authors examined the mRNA from the BALF lymphocytes , Kleiber et al 2005, macrophages (Laan et al 2005) and mononuclear cells (Ainsworth et al 2007, Reyner et al 2009) while other Authors (Franchini et al 2000, Bowles et al 2002, Giguère et al 2002, Ainsworth et al 2003, Debrue et al 2005) studied the cytokine network of all BALF cells. Moreover, it is necessary to realize that, in addition to the BALF cells, the bronchial epithelium is also capable of producing the cytokines involved in the development of acute RAO as reported by Bureau et al (2000) and by Ainsworth et al (2006), although a strict correlation between cytokine expression in BALF cells and in bronchial tissue has not been demonstrated (Riihimäki et al 2008a).…”

Section: Discussionmentioning

confidence: 99%

“…A real time PCR assay using the Syber Green method was carried out in a final volume of 25 μl containing 1X of SYBR PREMIX Ex Taq All the data are expressed as the ratio of the copies of the target gene and the copies of the housekeeping gene β actin, as endogenous control that is constitutively expressed in cells (Bowles et al 2002).…”

Section: Quantitative Real-time Pcr Analysismentioning

confidence: 99%

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Time-dependent changes of cytokines mRNA in bronchoalveolar lavage fluid from symptomatic Recurrent Airway Obstruction-affected horses

Pietra¹,

Cinotti²,

Ducci³

et al. 2011

Polish Journal of Veterinary Sciences

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During an 18 day test, we measured the cytokine mRNA expression (Interleukin-1β , , Interferon-γ [IFN-γ], Tumor Necrosis Factor-α [TNF-α]) of cells from bronchoalveolar lavage fluid [BALF] in five horses previously diagnosed with RAO, before and during challenge exposure, and after the desensitization phase which involved dexamethasone treatment and environmental modification. Simultaneously, the same cytokine mRNA expression of cells from BALF in four asymptomatic RAO-affected horses maintained outdoors was analyzed. An evident respiratory distress was observed in the challenge group within 3 days, with a significant overexpression of IL-8 and TNF-α mRNA on the ninth day. The pharmacological and environmental desensitization provided a down regulation of all the cytokines. No statistical modification characterized the cytokine kinetics of the asymptomatic horses maintained outdoors. A comparison for each time point of the cytokines between the exposed and unexposed horses showed no significant differences. The study suggested that a standardized exposure protocol and sampling time in experimental studies of RAO is mandatory for a correct comparison of the results obtained by different Authors. However, the absence of significant changes between the exposed and unexposed horses could depend on the lack of the sample uniformity since the evolution of the disease represents a continuum from a healthy to a pathological condition.

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“…Dieses erfolgte nach dem Protokoll von Bowles et al (2002), wobei zunächst 10 mg Ovalbumin in 225 mg Aluminium in die lange Sitzbeinmuskulatur injiziert wurden. Nach jeweils zwei und vier Wochen wurde die Reaktion durch eine 2 x 5 tägige Inhalation von 120 ml 0,125%iger Ovalbumin-Lösung über einen Ultraschall-Vernebler geboostert (Air One, Hippomed/Neu-Tech GmbH, Deutschland).…”

Section: Materials Und Methodeunclassified

Pilot study on passive sensitization against ovalbumin using equine Precision Cut Lung Slices (PCLS)

Barton¹,

Niedorf²,

Rohwer³

et al. 2010

PHK

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A novel model for equine recurrent airway obstruction

Cited by 28 publications

References 23 publications

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

A comparison of nanoparticulate CpG immunotherapy with and without allergens in spontaneously equine asthma‐affected horses, an animal model

Time-dependent changes of cytokines mRNA in bronchoalveolar lavage fluid from symptomatic Recurrent Airway Obstruction-affected horses

Pilot study on passive sensitization against ovalbumin using equine Precision Cut Lung Slices (PCLS)

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