A novel mode of stimulating platelet formation activity in megakaryocytes with peanut skin extract

Abstract

We report in this study novel biochemical activities of peanut skin extract (PEXT) on thrombocytopoiesis. Peanut skin, derived from Arachis hypogaea L., is a traditional Chinese medicine that is used to treat chronic hemorrhage. We have shown that oral administration of PEXT increases the peripheral platelet levels in mice. Recently, we reported a liquid culture system that is useful for investigating megakaryocytopoiesis and thrombocytopoiesis from human CD34 cells. In this liquid culture system, PEXT was shown to enhance the formation of CD41/DAPI cells (platelets), but had no effect on the formation of CD41/DAPI cells (megakaryocytes) or on the DNA content. Furthermore, PEXT selectively stimulated proplatelet formation from cultured mature megakaryocytes and phorbol 12-myristate 13 acetate (PMA)-induced formation of platelet-like particles from Meg01 cells. Despite having no influence on the formation of megakaryocyte colony forming units (CFUs), PEXT increased the size of megakaryocytes during their development from CD34 cells. PEXT showed no effect on the GATA-1 and NF-E2 mRNA levels, which are known to play an important role in thrombocytopoiesis and, based on the results of a pMARE-Luc (pGL3-MARE-luciferase) assay, had no influence on NF-E2 activation in Meg01 cells. These results suggest that PEXT accelerates proplatelet formation from megakaryocytes but does not influence the development of hematopoietic stem cells into megakaryocytes.

Extracts of Peanut Skins as a Source of Bioactive Compounds: Methodology and Applications

“…Extraction of peanut skins has become commercialized to provide material for further studies. Aqueous ethanol without further definition was described as the solvent for the extraction of peanut skins for a study of the effect of the material on the formation of blood platelets in an animal model [96]. The goal of the study was to elucidate the mechanism of the observed action on hemorrhage described by traditional Chinese medical practice.…”

Chinese Medicine in Cancer Treatment – How is it Practised in the East and the West?

“…Although clinical trials are lacking, there is a pre-clinical study supporting its use. One Japanese group [50] recently found that peanut skin extract accelerated proplatelet formation from megakaryocytes in a liquid cell culture system of CD34 human cells. Two other common 'anti-cancer' herbs in Chinese societies are Ganoderma lucidum (Ling Zhi) and Cordyceps sinensis.…”

Use of agro‐industrial residues as potent antioxidant, antiglycation agents, and α‐amylase and pancreatic lipase inhibitory activity

“…Vegetable residues derived from agro‐industry, such as peanut skin (PS) and grape pomace (GP) from wine production, are generally discarded, despite the great potential of use and transformation of this biomass into high value‐added products (de Camargo, Regitano‐d'Arce, Biasoto, & Shahidi, ; Sato et al, ). Vegetal residues can be transformed into by‐products or even agro‐industrial products, closing the production cycle, meeting environmental requirements and the production standards that are in line with the concepts of circular economy.…”