A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method

Hjorth-Sørensen, Bjørn; Pallisgaard, Niels; Grönholm, Mikaela; Hokland, Peter; Clausen, Niels; Jørgensen, Poul

doi:10.1038/sj.leu.2400758

Cited by 18 publications

(12 citation statements)

References 2 publications

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“…[8][9][10][11] Non-random chromosomal rearrangements involving chromosomes 10 and 11 were identified by cytogenetic analysis, but with the type of rearrangements and breakpoints being variable. A significant proportion of these 10;11 abnormalities was shown to result in MLL-MLLT10 fusion, [8][9][10][11][12][13][14][15][16][17] although the occurrence of two other possible transcripts, CALM-MLLT10 and MLL-ABI1 has been described. 10,[18][19][20] The nature and complexity of these 10;11 rearrangements was first described by Beverloo et al 8 and explained by the opposite orientation of both genes on the respective chromosome arms.…”

Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia

et al. 2002

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MLLT10(previously called AF10) is a moderately common MLL fusion partner predominantly occurring in acute monoblastic leukemia (AML-M5). 10;11 rearrangements require at least three breaks in order to generate an in-frame MLL-MLLT10 fusion as a result of the opposite orientations of both genes on the respective chromosome arms. In this study, we describe a detailed molecular cytogenetic analysis of MLL-MLLT10 positive 10;11 rearrangements in two patients. We observed an as yet unreported chromosomal mechanism with at least four breakpoints, leading to MLL-MLLT10 gene fusion in a 24-yearold male. An inversion of 11q13-q23 with a breakpoint in the MLL gene was followed by an additional break 3′ of MLL prior to insertion of the 11q segment into MLLT10. In a second patient, a 37-year-old male with AML-M5b, molecular cytogenetic analysis of an apparent 10;11 reciprocal translocation showed an intrachromosomal inversion of 3′MLLT10 followed by a reciprocal translocation between 10p12 and 11q23. Review of the literature showed that all cases were the result of an inversion of either 10p or 11q followed by translocation 10p;11q or insertion of the inverted segment into MLLT10 or MLL.

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Section: Introductionmentioning

confidence: 99%

Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia

et al. 2002

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“…The fusion occurred at breakpoints already described for the partner genes AF10 and AF6q27. 6,7 It will be noted that the contribution of the MLL protein to the fusion product was larger for P2 and P3 than in other cases described to date. It included, in particular, the major part of the Drosophila trithorax zinc-fingers domain of the MLL protein.…”

Section: To the Editormentioning

confidence: 91%

Recurrent involvement of the MLL gene in adult T-lineage acute lymphoblastic leukemia

Hayette

Tigaud

Maguer-Satta

et al. 2002

Blood

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human RANKL we used for immunocytochemistry and its isotype control (mouse IgG2b), and fluorochrome conjugated F(abЈ) 2 fragment goat anti-mouse IgG. Using flow cytometry, we could detect a strong expression of RANKL on bone marrow plasma cells in all multiple myeloma patients investigated (Figure 2). Furthermore, we performed Western blot analysis in 6 human myeloma cell lines (IM-9, NCI-H929, LP-1, OPM-2, RPMI 8226, U266) and found RANKL protein expression in all of them.These results extend our recent findings using flow cytometry alone in 10 previous patients, 6 underline that RANKL is directly expressed by plasma cells in the bone marrow of multiple myeloma patients with osteolytic bone lesions, and suggest a direct role of this ligand in the osteoclast activation in multiple myeloma.

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“…It contains an NH 2 -terminal A-T hook DNA binding domain, a methyltransferase-like domain, and a COOH-terminal trithorax-like region composed of a transcriptional activation domain and several contiguous zinc fingers (69)(70)(71)(72). The MLL gene is a recurring target for translocation in a variety of clinically distinct leukemias, and several other translocation partners of MLL have been cloned (69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79). The ELL protein is the only partner of MLL whose function is known (4)(5)(6).…”

Section: The Ell Family Of Rna Polymerase II Elongation Factors and Hmentioning

confidence: 99%

Factors regulating the transcriptional elongation activity of RNA polymerase II

Shilatifard

1998

FASEB j.

104

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The synthesis of mature and functional messenger RNA by eukaryotic RNA polymerase II (Pol II) is a complex, multistage process requiring the cooperative action of many cellular proteins. This process, referred to collectively as the transcription cycle, proceeds via five stages: preinitiation, initiation, promoter clearance, elongation, and termination. During the past few years, fundamental studies of the elongation stage of transcription have demonstrated the existence of several families of Pol II elongation factors governing the activity of Pol II. It is now clear that the elongation stage of transcription is a critical stage for the regulation of gene expression. In fact, two of these elongation factors, ELL and elongin, have been implicated in human cancer. This article will review the proteins involved in the regulation of the elongation stage of transcription by Pol II, describing the recent experimental findings that have propelled vigorous research on the properties and function of the elongating RNA polymerase II. --Shilatifard, A. Factors regulating the transcriptional elongation activity of RNA polymerase II.

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A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method

Abstract: A novel variant of the chimerical MLL-AF10 mRNA transcript method developed from an asymmetrical PCR method was detected in a pediatric patient with acute myeloid leukemia previously described by us.

Cited by 18 publications

References 2 publications

Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia

Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia

Recurrent involvement of the MLL gene in adult T-lineage acute lymphoblastic leukemia

Factors regulating the transcriptional elongation activity of RNA polymerase II

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