A novel microfluidic system to separate sperm using spermatozoa inherent motion and inertial effect

Panah, Anahita Ghassemi; Targhi, Mohammad Zabetian; Heydari, Ali; Halvaei, Iman

doi:10.1016/j.jbiomech.2022.111256

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Moreover, spiral microchips separate sperm from round cells, such as RBCs and WBCs, merely based on their physical properties (i.e. density and diameter) 38 . Also, the biological entities accumulated inside their outlets are not 100% pure.…”

Section: Introductionmentioning

confidence: 99%

A novel microfluidic device with parallel channels for sperm separation using spermatozoa intrinsic behaviors

Heydari

Targhi

Halvaei

et al. 2023

Sci Rep

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Isolating high-quality motile sperm cells is considered to be the main prerequisite for a successful artificial pregnancy. Microfluidics has emerged as a promising platform capable of mimicking in-vivo environments to separate motile sperm cells and bypassing the need for the current invasive clinical sperm separation methods. In this study, the proposed microfluidic device exploits the parallelization concept through symmetry to increase both the processed sample volume and the injected flow rate compared with the previous conventional devices, which used rheotaxis as their primary method of sperm separation. Using the finite element method (FEM) and flow simulations, the trajectories of sperm cells exhibiting rheotaxis behavior were predicted inside the proposed device. Different flow rates, including 0, 0.5, 1.5, 3, 4.5 and 6 μl/min, were experimentally injected into the device, and the effect of flow rate on the size of the hypothetical rheotaxis zone and the number of isolated sperm cells was investigated. Furthermore, it was illustrated that 100% of the isolated motile sperm cells are motile, and by manipulating the injected flow rate into the device, different classes of sperm cells in terms of motility parameters can be separated and utilized for further uses.

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Section: Introductionmentioning

confidence: 99%

A novel microfluidic device with parallel channels for sperm separation using spermatozoa intrinsic behaviors

Heydari

Targhi

Halvaei

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Spermatozoa culture can be obtained from spermatogonial stem cells, testicular spermatogenic cells and direct culture of spermatozoa cells [41] , in which the former two only stays in the laboratory stage and the last gets mature application at present [42] . High quality sperm can be obtained through spermatozoa culture in vitro, which can not only improve the success rate of ART for pregnancy, but also help to improve the method of ART for pregnancy [43] , and reduce medical costs and risks. There are many kinds of spermatozoa culture medium on the market can be chose.…”

Section: Discussmentioning

confidence: 99%

The effects of L-carnitine and fructose in improved Ham’s F10 on sperm culture in idiopathic severe asthenospermia within 24h

Chang,

Li,

Kang

et al. 2024

Preprint

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Trial designTo study the effects of L-carnitine and fructose on semen parameters of severe asthenospermia patients by sperm culturing in vitro within 24h. We optimized the energy composition and antioxidant substances of sperm culture medium in vitro (based on Ham’s F10 culture medium) by orthogonal test for preparing high quality culture medium. Sperms of 60 patients with idiopathic severe asthenospermia were collected, and cultured in vitro within 24h, by Ham’s F10 culture medium added to different concentrations of L-carnitine and fructose and culture temperature, whose effects on sperm motility were observed to determine which is the most appropriate concentration and temperature.MethodsFor determining the appropriate concentration of L-carnitine and fructose and the suitable culture temperature in Ham’s F10 culture medium, the orthogonal experiments were carried out to optimize above three factors, which had great influence on sperm viability, survival rate, deformity rate and DNA fragmentation index (DFI).ResultsThe final concentration of L-carnitine and fructose was determined in terms of initial tests to assess the effects of different concentrations (4, 8, 12, and 16 mg/ml L-carnitine and 0.125, 0.250, 0.375, and 0.50 mg/ml fructose) on sperm viability and motility in culture. During the operation of processing and culturing sperms in vitro within 24h, orthogonal test showed that sperm viability was better at the final concentration of 8 mg/ml L-carnitine and 0.375 mg/mL fructose in improved Ham’s F10 culture medium at 36.5 ℃.ConclusionIdiopathic severe asthenospermia sperm can be effectively improved by the modified Ham’s F10 culture medium of the final concentration of 8 mg/ml L- carnitine and 0.375 mg/mL fructose in at 36.5 ℃ within 24h, which has shown better culture effect and is superior to Ham’s F10 basic medium.

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Qualified sperm selection based on the rheotaxis and thigmotaxis in a microfluidic system

et al. 2023

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A novel microfluidic system to separate sperm using spermatozoa inherent motion and inertial effect

Cited by 4 publications

References 18 publications

A novel microfluidic device with parallel channels for sperm separation using spermatozoa intrinsic behaviors

A novel microfluidic device with parallel channels for sperm separation using spermatozoa intrinsic behaviors

The effects of L-carnitine and fructose in improved Ham’s F10 on sperm culture in idiopathic severe asthenospermia within 24h

Qualified sperm selection based on the rheotaxis and thigmotaxis in a microfluidic system

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