A novel microfluidic immunoassay system based on electrochemical immunosensors: An application for the detection of NT-proBNP in whole blood

Electrochemical Biosensors for the Determination of Cardiovascular Markers: a Review

“…NT‐proBNP detection was based on changes in hexanocyanoferrate solutions cyclic voltammograms without or with Ag‐Ab reaction. This system constituted the basis for a sandwich‐configuration immunosensor developed by the same group 22 (see Section 2.3. ).…”

“…Liang et al. 22 recently described an amperometric regeneration‐free immunosensor for the determination of NT‐proBNP combined with a microfluidic system which enabled continuous determination. Avidin‐modified magnetic nanoparticles were incubated in a mixture of biotinylated capture antibody, the sample and a secondary antibody labeled with a Pt and Prussian blue nanomaterial (PPN).…”

Advances in the detection and quantification of candidate and established biomarkers in heart failure

“…New, simpler, cost-effective methods for BNP detection such as the immunosensors are being tested as candidates for the detection of NTproBNP. They detect NT-proBNP in serum such as the regeneration-free immunosensor with novel Fab fragment monoclonal antibodies that detects NT-proBNP from 0.04 to 2.5 ng/ml with a limit of 0.03 ng/ml (12) or in whole blood such as the combination of a microfluidic system with an immunoassay based on an electrochemical immunosensor with magnetic nanoparticles, biotin-avidin system (BAS) and fragment antigen binding (Fab) antibodies (13) or the electrochemical immunosensor based on the nanostructural gold and carbon nanotubes composite, gold nanochains (AuNCs) and horseradish peroxidase (HRP) complex labeled secondary antibodies (AuNCs-HRP-Ab(2)) (14).…”

Immunodiagnostics and Immunosensor Design

“…Currently, the standard control of the reagent flow is done by intelligent microfluidic systems with micropumps and valves. However, the need for novel, low-resource fabrication and assembly methods for creating disposable detectors leads to the development of alternative microfluidics concepts such as hybrid paper-polymer devices [102], centrifugal micro- [103], and regeneration-free immunosensors [104]. The recent surge in the development of microfluidics systems was fuelled by research advances in polymer science.…”