A novel microfluidic aqueous two-phase system with immobilized enzyme enhances cyanidin-3-O-glucoside content in red pigments from mulberry fruits

Zhou, Xuejiao; Zhu, Chang‐Tong; Hu, Yan; You, Shuai; Wu, Fu‐An; Wang, Jun

doi:10.1016/j.bej.2020.107556

Cited by 15 publications

(3 citation statements)

References 42 publications

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“…The content of C3G in blood-flesh peach is much higher than that of D3G ( Figure 3 B,C). This result was consistent with the literature that C3G accounts for most of the anthocyanins in peach [ 30 ].…”

Section: Resultssupporting

confidence: 93%

Effects of Hot Air Drying on Drying Kinetics and Anthocyanin Degradation of Blood-Flesh Peach

Tan

Miao

Zhou

et al. 2022

Foods

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The purpose of this study was to explore the drying kinetics, effective moisture diffusivity, activation energy, color variation, and the thermal degradation properties of anthocyanins of blood-flesh peach under hot air drying for the first time. The results showed that the hot air-drying process of blood-flesh peach belongs to reduced-speed drying. The Page model could accurately predict the change of moisture ratio of blood-flesh peach. The effective moisture diffusivity during hot air drying of blood-flesh peach was in the range between 1.62 × 10−10 and 2.84 × 10−10 m2/s, and the activation energy was 25.90 kJ/mol. Fresh samples had the highest content (44.61 ± 4.76 mg/100 g) of total monomeric anthocyanins, and it decreased with the increase of drying temperature. Cyanidin-3-O-glucoside and delphinidin-3-O-galactoside were the main anthocyanins of blood-flesh peach as identified and quantified by UPLC-QqQ-MS. Interestingly, during the drying process, the content of cyanidin-3-O-glucoside increased at the beginning, and then decreased. However, the content of delphinidin-3-O-galactoside kept decreasing during the whole drying process. Considering the drying efficiency, fruit color and quality, 70 °C would be a suitable temperature for drying blood-flesh peach. This research will provide beneficial information for understanding the anthocyanin degradation of blood-flesh peach during drying, and guide the production of high-quality dried products.

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Section: Resultssupporting

confidence: 93%

Effects of Hot Air Drying on Drying Kinetics and Anthocyanin Degradation of Blood-Flesh Peach

Tan

Miao

Zhou

et al. 2022

Foods

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“…Quercetin-3-O-arabinoside, Quercetin-3-O-rhamnoside (Quercitrin) and Isorhamnetin-3-O-sophoroside are important flavonol metabolites in the color change process of pepper. Flavonoids usually found in epidermal tissue, are the main molecules involved in plant pigmentation and accumulate through various modes during fruit development [ 33 , 34 ]. The metabolites of flavonoids and carotenoids mainly were up-regulated during the yellow-orange fruit period, while most of the metabolites of flavonoids and carotenoids were down-regulated during the orange-red fruit period, suggesting that the types and contents of metabolites differed during the different developmental stages of pepper fruit.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analysis of Color Changes during Fruit Development of Pepper (Capsicum baccatum)

Shu

Mumtaz

Hao

et al. 2022

IJMS

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Fruit color is one of the most critical characteristics of pepper. In this study, pepper (Capsicum baccatum L.) fruits with four trans-coloring periods were used as experimental materials to explore the color conversion mechanism of pepper fruit. By transcriptome and metabolome analysis, we identified a total of 307 flavonoid metabolites, 68 carotenoid metabolites, 29 DEGs associated with flavonoid biosynthesis, and 30 DEGs related to carotenoid biosynthesis. Through WGCNA (weighted gene co-expression network analysis) analysis, positively correlated modules with flavonoids and carotenoids were identified, and hub genes associated with flavonoid and carotenoid synthesis and transport were anticipated. We identified Pinobanksin, Naringenin Chalcone, and Naringenin as key metabolites in the flavonoid biosynthetic pathway catalyzed by the key genes chalcone synthase (CHS CQW23_29123, CQW23_29380, CQW23_12748), cinnamic acid 4-hydroxylase (C4H CQW23_16085, CQW23_16084), cytochrome P450 (CYP450 CQW23_19845, CQW23_24900). In addition, phytoene synthase (PSY CQW23_09483), phytoene dehydrogenase (PDS CQW23_11317), zeta-carotene desaturase (ZDS CQW23_19986), lycopene beta cyclase (LYC CQW23_09027), zeaxanthin epoxidase (ZEP CQW23_05387), 9-cis-epoxycarotenoid dioxygenase (NCED CQW23_17736), capsanthin/capsorubin synthase (CCS CQW23_30321) are key genes in the carotenoid biosynthetic pathway, catalyzing the synthesis of key metabolites such as Phytoene, Lycopene, β-carotene and ε-carotene. We also found that transcription factor families such as p450 and NBARC could play important roles in the biosynthesis of flavonoids and carotenoids in pepper fruits. These results provide new insights into the interaction mechanisms of genes and metabolites involved in the biosynthesis of flavonoids and carotenoids in pepper fruit leading to color changes in pepper fruit.

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“…ATPS are a novel separation and extraction technology with higher water content, lower interfacial tension, non-toxic, environmentally friendly comparing with traditional liquid-liquid extraction [1][2][3]. It has been extensively studied in the extraction and purification of downstream products, for example, nanoparticles [4], proteins [5], antibiotics [6], biological molecules [7].…”

Section: Introductionmentioning

confidence: 99%

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Wei

Chen

Yang

et al. 2021

Appl Biochem Biotechnol

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Aqueous two-phase systems (ATPS) have the advantages of environmentally friendly, high mass transfer efficiency, mild extraction conditions. However, it is difficult to recycle these components, which limits the large-scale application of ATPS in industrial production. In this study, a novel recyclable ATPS of PN/PADB4.78 was constructed with a thermo-responsive polymer PN and a pH-responsive polymer PADB4.78 for the partition study of tea saponin. The recovery of PN reached 98.71% by adjusting the temperature to above its lower critical solution temperature (LCST, 33.0 °C ) and the recovery of PADB4.78 was 97.32% by regulating the pH to its isoelectric point (pI, 4.78). Meanwhile, the phase formation mechanism of ATPS was studied by surface tension and low-field nuclear magnetic resonance (LF-NMR). The effects of polymer concentration, pH, temperature, types and concentrations of salt were investigated on tea saponin partition.In the 1.5% PN/3.5% PADB4.78 ATPS, the optimal partition coefficient (K) and extraction recovery (ERb) of crude tea saponin were 0.15 and 92.13% in the presence of 1.5 mM KCl at pH=7.6 and 25℃, respectively. The K and ERb of tea saponin from tea seeds were 0.12 and 94.50% with 7.5 mM LiBr at pH=8.0 and 25℃, respectively.

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A novel microfluidic aqueous two-phase system with immobilized enzyme enhances cyanidin-3-O-glucoside content in red pigments from mulberry fruits

Cited by 15 publications

References 42 publications

Effects of Hot Air Drying on Drying Kinetics and Anthocyanin Degradation of Blood-Flesh Peach

Effects of Hot Air Drying on Drying Kinetics and Anthocyanin Degradation of Blood-Flesh Peach

Transcriptome and Metabolome Analysis of Color Changes during Fruit Development of Pepper (Capsicum baccatum)

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

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