Abstract:Chloroflexales (Chloroflexi) are typical members of the anoxygenic photosynthesizing component of microbial mats and have mostly been characterized from communities associated to hot springs. Here, we report the assembly of five metagenome-assembled genomes of a novel lineage of Chloroflexales found in mesophilic lithifying microbial mats (microbialites) in Lake Alchichica (Mexico). Genomic and phylogenetic analyses revealed that the bins shared 92% of their genes, and these genes were nearly identical despite… Show more
“…Acinetobacter , Acidobacteria , Oxyphotobacteria . Chloroflexi is a typical member of the anaerobic photosynthetic taxa, mostly from hot spring-associated environments 26 . Figure 3 Venn distribution of a genus-level for all sample taxa.…”
Currently, investigations on the microbiota of sports centers and related facilities have been carried out in some countries, which showed that Microsporum gypseum, Trichophyton mentagrophytes and T. tonsurans are important dermatoprotofungi. In China, some research on athletes and sports equipment between the fungal community and public health has made some interesting achievements. However, the bacterial group among them has not been reported. Therefore, The aim of this study was to uncover (I) gymnastic equipment is there potential pathogenic factors and (ii) is there any difference in the biomarker of bacterial in different types of gymnastic room? The samples were collected from the gymnastics halls of one university in western China and main sports equipment, including gymnastics carpets, moving barres, hoops and balls, as well as wall bars, parallel bars and horizontal bars. The 16S rDNA of all the samples was sequenced, and the analyses were performed using FaproTax, Bug base function prediction and Line Discriminant Analysis (LDA) Effect Size. A 16S rDNA sequence analysis revealed abundant bacterial species biodiversity on gymnasts and apparatuses from two gymnastics halls at a university in western China. An analysis using the FaproTax and Bugbase functional prediction platforms showed that there were some opportunistic pathogens on the athletes and equipment from the Rhythmic Gymnastics (RG) and Artistic Gymnastics (AG) halls, such as Staphylococcus and Corynebacteiaceae. Infectious agents associated with cancer induction and development, such as Ruminococcaceae, Veillonellaceae and Moraxellaceae, as well as microbial toxin producers with a potential impact on human health, were also detected. According to a line discriminant analysis (LDA effect size), the bacterial biomarker groups of the two gymnasiums were different at the phylum-genus level: for RG, Erysipelatoclostridium, Lachnospiraceae and Bacteroidales, while for AG, Rhizobiales. Based on the results of the investigation, we suggest that more comprehensive consideration should be given to indoor microbial biodiversity and related public health problems in school gymnasiums.
“…Acinetobacter , Acidobacteria , Oxyphotobacteria . Chloroflexi is a typical member of the anaerobic photosynthetic taxa, mostly from hot spring-associated environments 26 . Figure 3 Venn distribution of a genus-level for all sample taxa.…”
Currently, investigations on the microbiota of sports centers and related facilities have been carried out in some countries, which showed that Microsporum gypseum, Trichophyton mentagrophytes and T. tonsurans are important dermatoprotofungi. In China, some research on athletes and sports equipment between the fungal community and public health has made some interesting achievements. However, the bacterial group among them has not been reported. Therefore, The aim of this study was to uncover (I) gymnastic equipment is there potential pathogenic factors and (ii) is there any difference in the biomarker of bacterial in different types of gymnastic room? The samples were collected from the gymnastics halls of one university in western China and main sports equipment, including gymnastics carpets, moving barres, hoops and balls, as well as wall bars, parallel bars and horizontal bars. The 16S rDNA of all the samples was sequenced, and the analyses were performed using FaproTax, Bug base function prediction and Line Discriminant Analysis (LDA) Effect Size. A 16S rDNA sequence analysis revealed abundant bacterial species biodiversity on gymnasts and apparatuses from two gymnastics halls at a university in western China. An analysis using the FaproTax and Bugbase functional prediction platforms showed that there were some opportunistic pathogens on the athletes and equipment from the Rhythmic Gymnastics (RG) and Artistic Gymnastics (AG) halls, such as Staphylococcus and Corynebacteiaceae. Infectious agents associated with cancer induction and development, such as Ruminococcaceae, Veillonellaceae and Moraxellaceae, as well as microbial toxin producers with a potential impact on human health, were also detected. According to a line discriminant analysis (LDA effect size), the bacterial biomarker groups of the two gymnasiums were different at the phylum-genus level: for RG, Erysipelatoclostridium, Lachnospiraceae and Bacteroidales, while for AG, Rhizobiales. Based on the results of the investigation, we suggest that more comprehensive consideration should be given to indoor microbial biodiversity and related public health problems in school gymnasiums.
“Filamentous anoxygenic phototrophs” or “green non-sulfur bacteria” form a distinct lineage, order Chloroflexales (Chloroflexia: Chloroflexi) in broad polyphyletic group of anoxygenic phototrophic bacteria. Filamentous anoxygenic phototrophic bacteria have apparently important, though not fully understood functions in contemporary ecosystems and perhaps were much more significant in the Archaean. However, since their discovery in hot spring mats in the late 1960s, the extremophilic species of thermal, hypersaline and/or hyperalcaline habitats remain the most studied representatives of the lineage. In this overview, we show our representation of the history of finding and research of filamentous anoxygenic phototrophs, and current situation with their taxonomy, phylogeny and diversity. Some problems and uncertainties in these fields are also discussed. The special section is dedicated to the discovery and research of this group in the Volga River basin. Due to the small number of studies, the exact distribution of filamentous anoxygenic phototrophic bacteria in the region is unknown. For example, in small stratified lakes with high humic and/or ferruginous water in the basins of the Oka and the Upper Volga the development of planktonic filamentous anoxygenic phototrophs is very likely. Besides, the development of Chloroflexales, including new species, is quite expected in the microbial mats of saline rivers and hypersaline lakes of the Lower Volga region. Further progress in the field will be possible only with the use of molecular methods along or preferably in combination with traditional methods (polyphasic approach). Since their role in communities is far from elucidated, further studies of FAP may also provide new opportunities in the field of microbial biotechnology.
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