A novel method to produce triploids in bivalve molluscs by the use of 6-dimethylaminopurine

Desrosiers, Richard R.; Gérard, André; Peignon, Jean-Marie; Naciri, Yamama; Dufresne, Louise; Morasse, Julie; Ledu, Christophe; Phélipot, Pascal; Galle, P.; Dubé, François

doi:10.1016/0022-0981(93)90127-a

Cited by 62 publications

(50 citation statements)

References 27 publications

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“…Marthasterias glacialis) (Desrosiers et al, 1993). When applied soon after fertilization, 6-DMAP reversibly inhibits nuclear envelope breakdown and the mitotic apparatus (Dufresne et al, 1991).…”

Section: Accepted Manuscriptmentioning

confidence: 97%

“…When applied soon after fertilization, 6-DMAP reversibly inhibits nuclear envelope breakdown and the mitotic apparatus (Dufresne et al, 1991). In various experiments, 6-DMAP has been found to act on specific protein kinases, promote chromatin decondensation, and act on microtubules and metaphase spindles in mouse oocytes and sea urchin embryos (Desrosiers et al, 1993;Gérard et al, 1999). In mammals, 6-DMAP has been used to activate pronuclear formation in fertilized eggs (Leal and Liu, 1998).…”

Section: Accepted Manuscriptmentioning

confidence: 98%

“…Comparisons between C. gigas triploids induced by CB and by 6-DMAP revealed that the treatments yield similar numbers of triploids and comparable survival rates (Desrosiers et al, 1993;Gérard et al, 1999). Handling 6-DMAP is significantly safer for hatchery workers than…”

Section: Introductionmentioning

confidence: 95%

“…Chemical treatment yields variable results for a number of reasons (Allen et al, 1989). CB is labeled a toxin and a potential workplace hazard; it can be fatal through ingestion, inhalation, or through contact with skin (Sigma-Aldrich, 2015b) and is suspected of damaging fertility (Desrosiers et al, 1993). In 2000, Eudeline et al optimized a tetraploid induction technique using CB.…”

Section: Introductionmentioning

confidence: 98%

“…In order to compensate for differences in egg quality, Eudeline et al (2000) developed the biological clock method that relies on observation of the extrusion of polar bodies over time in order to effectively administer treatments. Desrosiers et al (1993) examined an alternative to CB for inducing triploidy using the protein kinase inhibitor 6-dimethylaminopurine (6-DMAP) (Szöllösi et al, 1993) instead of CB.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica

Peachey

Allen

2016

Aquaculture

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Please cite this article as: Peachey, Brittany L., Allen Jr., Standish K., Evaluation of cytochalasin B and 6-dimethylaminopurine for tetraploidy induction in the Eastern oyster, Crassostrea virginica, Aquaculture (2015), AbstractCytochalasin B (CB) has been used to induce tetraploidy in oysters since the practice began in 1993. However, CB is toxic and presents health risks to hatchery workers who administer the treatment. 6-dimethylaminopurine (6-DMAP) is also an effective cytokinetic inhibitor, and does not carry the health risks of CB. We examined the relative effectiveness of 6-DMAP vs CB for producing tetraploids in the Eastern oyster (Crassostrea virginica). Survival and yield of tetraploids varied widely among the 15 experiments. Larvae resulting from 6-DMAP treatment had higher survival in 11 of the 14 trials on day two and day six/ seven. For yield of tetraploids, 10 of 13 6-DMAP treatments had higher proportions of tetraploids on day two and at the second sampling -day six, seven, or nine -7 of 10 had higher proportions of tetraploids. Tetraploid spat were obtained from the majority of surviving cultures. Based on these results, 6-DMAP can effectively replace CB for inducing polyploidy in C. virginica, and probably other Crassostrea spp., due to the success of the treatment, the ease of application, and the reduction in health risk to hatchery workers. This study set the precedent for the use of 6-DMAP on C. virginica and A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT 2 established a new procedure for inducing tetraploids using triploid eggs. It might be possible to refine the treatment to further optimize yield of tetraploids.

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Section: Accepted Manuscriptmentioning

confidence: 97%