A novel method to prepare L-Arabinose from xylose mother liquor by yeast-mediated biopurification

Abstract

BackgroundL-arabinose is an important intermediate for anti-virus drug synthesis and has also been used in food additives for diets-controlling in recent years. Commercial production of L-arabinose is a complex progress consisting of acid hydrolysis of gum arabic, followed by multiple procedures of purification, thus making high production cost. Therefore, there is a biotechnological and commercial interest in the development of new cost-effective and high-performance methods for obtaining high purity grade L-arabinose.ResultsAn alternative, economical method for purifying L-arabinose from xylose mother liquor was developed in this study. After screening 306 yeast strains, a strain of Pichia anomala Y161 was selected as it could effectively metabolize other sugars but not L-arabinose. Fermentation in a medium containing xylose mother liquor permitted enrichment of L-arabinose by a significant depletion of other sugars. Biochemical analysis of this yeast strain confirmed that its poor capacity for utilizing L-arabinose was due to low activities of the enzymes required for the metabolism of this sugar. Response surface methodology was employed for optimization the fermentation conditions in shake flask cultures. The optimum conditions were: 75 h fermentation time, at 32.5°C, in a medium containing 21% (v/v) xylose mother liquor. Under these conditions, the highest purity of L-arabinose reached was 86.1% of total sugar, facilitating recovery of white crystalline L-arabinose from the fermentation medium by simple methods.ConclusionYeast-mediated biopurification provides a dynamic method to prepare high purity of L-arabinose from the feedstock solution xylose mother liqour, with cost-effective and high-performance properties.

Investigation of selective arabinose release from corn fibre by acid hydrolysis under mild conditions

“…On an industrial scale it is produced from gum arabic by acid hydrolysis followed by multiple purification procedures. The relatively high cost of gum arabic and the expensive purification steps result in the high cost of pure arabinose, which is around 50 EUR kg −1 . Therefore, there is biotechnological and commercial interest in the development of new cost‐effective methods for producing high purity grade L‐arabinose from lignocellulosic and pectin‐containing materials .…”

“…The relatively high cost of gum arabic and the expensive purification steps result in the high cost of pure arabinose, which is around 50 EUR kg −1 . Therefore, there is biotechnological and commercial interest in the development of new cost‐effective methods for producing high purity grade L‐arabinose from lignocellulosic and pectin‐containing materials . Different strategies investigated involve enzymatic hydrolysis of purified arabinan or arabinoxylan, biopurification of hemicellulosic hydrolysate and acidic hydrolysis of agricultural residues containing significant amount of arabinose under mild conditions .…”

Catabolite responsive element deficiency of xyl operon resulting in carbon catabolite derepression in Lactobacillus fermentum 1001

“…Arabinose is one of the three main carbohydrates in corncob hydrolysate and an important intermediate for anti‐virus drug synthesis and food additives for diets‐controlling (Cheng et al . ). Therefore, Lact.…”

Waste Xylose Mother Liquor Derived 3 D Graphene‐Like Porous Carbon with Ultrahigh Specific Capacitance and Energy Density for Supercapacitors

“…However, no more than 30% of the produced xylose can be separated during crystallization from the liquors, and most of the xylose and other sugars remain in the liquid as xylose mother liquor . Due to its complex composition, large number of inhibitors and low cost of sugar, the development and utilization of this by‐product has been limited so far . Instead of spending the energy to separate the components, it is better to use cheap biomass to prepare them.…”