A novel method to control the balance of skin microflora

Katsuyama, Masako; Yusuke, Kobayashi; Ichikawa, Hideyuki; Atsuko, Mizuno; Matsunaga, Kayoko; Kawashima, Makoto

doi:10.1016/j.jdermsci.2005.01.003

Cited by 62 publications

(10 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3-1 and Fig. 3-2 3,4. Indeed, the treatment with FX cream improved significantly 4 out of 5 items in AD lesions at 2 weeks later, while the treatment with control cream improved only 1 item of dryness/desquamation as shown in Fig.…”

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 80%

“…The therapeutic effect of this FX cream is conceivable to be based on two functions of it, namely the prevention of adhesion of SA to the skin and the removal of SA from the skin. Therefore, this FX cream is expected as a useful skin care agent for atopic dry skin colonized by SA 3,4. Recently, we also found that AD-like lesions in DS-Nh of AD model mouse mice were significantly improved together with a decrease in number of SA detected from these lesions by treatment with the bamboo leaf extract cream having the antibacterial, viral and mycotic activity 5.…”

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 86%

“…Both of the number of SA and the ratio of SA to total bacteria at the sites, to which the FX cream containing F and X had been applied, significantly decreased in 2 weeks later, accompanied with clinical improvement of AD, as shown in Fig. 5 3,4. The therapeutic effect of this FX cream is conceivable to be based on two functions of it, namely the prevention of adhesion of SA to the skin and the removal of SA from the skin.…”

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 89%

“…Indeed, the treatment with FX cream improved significantly 4 out of 5 items in AD lesions at 2 weeks later, while the treatment with control cream improved only 1 item of dryness/desquamation as shown in Fig. 4 3,4. Both of the number of SA and the ratio of SA to total bacteria at the sites, to which the FX cream containing F and X had been applied, significantly decreased in 2 weeks later, accompanied with clinical improvement of AD, as shown in Fig.…”

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 91%

See 3 more Smart Citations

A Role of Staphyococcus aureus, Interleukin-18, Nerve Growth Factor and Semaphorin 3A, an Axon Guidance Molecule, in Pathogenesis and Treatment of Atopic Dermatitis

Ikezawa

Komori

Ikezawa

et al. 2010

Allergy Asthma Immunol Res

View full text Add to dashboard Cite

Staphylococcus aureus (SA) is usually present not only in the skin lesions of atopic dermatitis (AD) but also in the atopic dry skin. SA discharges various toxins and enzymes that injure the skin, results in activation of epidermal keratinocytes, which produce and release IL-18. IL-18 that induces the super Th1 cells secreting IFN-γ and IL-13 is supposed to be involved in development of AD and its pathogenesis. Indeed, the number of SA colonies on the skin surface and the serum IL-18 levels in patients with AD significantly correlated with the skin scores of AD lesions. Also, there is strong positive correlation between the skin scores and serum IL-18 levels in DS-Nh mice (P<0.0001, r=0.64), which develop considerable AD-like legions when they are housed under conventional conditions, but develop skin legions with less severity and less frequency under specific pathogens free (SPF) conditions. Therefore, they are well-known as model mice of AD, in which SA is presumed to be critical factor for the development of AD lesions. Also, theses DS-Nh mice pretreated with Cy developed more remarkable AD-like lesions in comparison with non-treated ones. The levels of INF-r and IL-13 in the supernatants of the lymph node cell cultures stimulated with staphylococcal enterotoxin B (SEB) or ConA were increased in the Cy-treated mice, although the serum levels of total IgE were not. In this experiment, we revealed that Cy-treated mice, to which CD25 +CD4 + reguratory T cells taken from non-treated ones had been transferred, developed the AD-like legions with less severity and less number of SA colonies on the skin surface. Therefore, it is presumed that CD25 +CD4 + reguratory T cells might be involved in the suppression of super Th1 cells which are induced by IL-18 and are involved in the development of AD-like lesions rather than IgE production. The efficient induction of CD25 +CD4 + reguratory T cells is expected for the new type of treatment of AD. We also found that farnesol (F) and xylitol (X) synergistically inhibited biofilm formation by SA, and indeed the ratio of SA in total bacteria at sites to which the FX cream containing F and X had been applied was significantly decreased 1 week later, accompanied with improvement of AD, when compared with that before application and at placebo sites. Therefore, the FX cream is a useful skin-care agent for atopic dry skin colonized by SA. The nerve growth factor (NGF) in the horny layer (the horn NGF) of skin lesions on the cubital fossa was collected by tape stripping and measured using ELISA in AD patients before and after 2 and 4 weeks treatments. Simultaneously, the itch and eruptions on the whole body and on the lesions, in which the horn NGF was measured, were recorded, and also the peripheral blood eosinophil count, serum LDH level and serum total IgE level were examined. The level of NGF was significantly higher in AD patients than in healthy controls, correlated with the severity of itch, erythema, scale/xerosis, the eosinophil count and LDH level, and also significantly ...

show abstract

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 80%

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 86%

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 89%

Section: A Probable Role Of Staphylococcus Aureus (Sa) and Interleukimentioning

confidence: 91%

See 2 more Smart Citations

A Role of Staphyococcus aureus, Interleukin-18, Nerve Growth Factor and Semaphorin 3A, an Axon Guidance Molecule, in Pathogenesis and Treatment of Atopic Dermatitis

Ikezawa

Komori

Ikezawa

et al. 2010

Allergy Asthma Immunol Res

View full text Add to dashboard Cite

show abstract

“…21) Glycosaminoglycan is involved in P. gingivalis-mediated cell adhesion, and glucuronic acid is necessary to inhibit P. gingivalis adherence to host cells significantly. 22,23) PG-F2 and PG-HMW demonstrated marked inhibitory activity against the adhesion of P. gingivalis and A. actinomycetemcomitans to human oral epithelial cells in a dose-dependent manner.…”

mentioning

confidence: 99%