A novel method to assess airway function parameters in chronically instrumented, unrestrained guinea-pigs

Santing, Re; Meurs, Herman; Vandermark, Tw; Remie, R; Oosterom, W.; Zaagsma, Johan

doi:10.1016/0952-0600(92)90069-s

Cited by 47 publications

(67 citation statements)

References 20 publications

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“…Via an external saline-filled cannula, the intrapleural balloon catheter was connected to a pressure transducer (TXX-R; Viggo-Spectramed, Bilthoven, The Netherlands), and P pl was continuously measured using an online computer system. By use of a combination of flow measurement with a pneumotachograph implanted inside the trachea and pressure measurement with the intrapleural balloon catheter, it was previously shown that changes in P pl are linearly related to changes in airway resistance and hence can be used as a sensitive index for allergen-and histamineinduced bronchoconstriction (Santing et al, 1992). In this way, airway function can be monitored continuously and repeatedly for prolonged periods of time, whereas the animals are unaware of the measurements being taken.…”

Section: Measurement Of Airway Functionmentioning

confidence: 99%

“…These provocations were carried out in a specially designed perspex cage of 9 liters in which the guinea pigs could move freely, as previously described (Santing et al, 1992;Meurs et al, 2006). A DeVilbiss nebulizer (type 646) driven by an airflow of 8 l/min provided the aerosol with an output of 0.33 ml/min.…”

Section: Provocationsmentioning

confidence: 99%

“…Airway function was assessed by online measurement of pleural pressure (P pl ) under conscious and unrestrained conditions as described previously (Santing et al, 1992;Meurs et al, 2006). In short, a small fluid-filled latex balloon catheter was surgically implanted inside the thoracic cavity.…”

Section: Measurement Of Airway Functionmentioning

confidence: 99%

See 2 more Smart Citations

Bronchoprotection by Olodaterol Is Synergistically Enhanced by Tiotropium in a Guinea Pig Model of Allergic Asthma

Smit

Zuidhof

Bos

et al. 2013

J Pharmacol Exp Ther

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The novel once-daily b 2 -agonist bronchodilator drug olodaterol has recently been shown to be effective in patients with allergic asthma for .24 hours. An increased cholinergic tone common to these patients may decrease the effectiveness of b 2 -agonists. This could provide a rationale for combination therapy with olodaterol and the long-acting anticholinergic tiotropium to aim for a once-daily treatment regimen. In guinea pigs, we evaluated the protective effects of olodaterol, alone and in combination with tiotropium, on airway responsiveness to histamine, which is partially mediated by a cholinergic reflex mechanism. In addition, using a guinea pig model of acute allergic asthma, we examined the cooperative effects of these bronchodilators on allergen-induced early (EAR) and late (LAR) asthmatic reactions, airway hyper-responsiveness (AHR) to histamine, and airway inflammation. It was demonstrated that the protective effect of olodaterol against histamine-induced bronchoconstriction was synergistically enhanced and prolonged in the presence of tiotropium. In addition, tiotropium synergistically augmented both the reversal of and the protection against the allergeninduced AHR after the EAR by olodaterol. Olodaterol and tiotropium were highly effective in inhibiting the magnitude of the allergen-induced EAR and LAR, and both reactions were fully inhibited by the combination of these drugs. It is remarkable that these effects were not associated with an effect on inflammatory cell infiltration in the airways. In conclusion, the results indicate that combination therapy with olodaterol and tiotropium may be highly effective in the treatment of allergen-induced asthmatic reactions and AHR.

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Section: Measurement Of Airway Functionmentioning

confidence: 99%

Section: Provocationsmentioning

confidence: 99%

See 1 more Smart Citation

Bronchoprotection by Olodaterol Is Synergistically Enhanced by Tiotropium in a Guinea Pig Model of Allergic Asthma

Smit

Zuidhof

Bos

et al. 2013

J Pharmacol Exp Ther

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“…All animals were sensitised to ovalbumin (grade III ovalbumin; Sigma Chemical Co., St Louis, MO, USA) at 3 weeks of age as described previously [15]. In order to obtain a shift to IgE-class antibodies, an allergen solution containing 100 mg?mL -1 ovalbumin and 100 mg?mL -1 Al(OH) 3 (J.T.…”

Section: Animalsmentioning

confidence: 99%

“…Airway function was assessed by on-line measurement of pleural pressure (Ppl) under unrestrained conditions as described previously [15]. In brief, a small fluid-filled latex balloon connected to a saline-filled cannula was surgically implanted inside the thoracic cavity.…”

Section: Measurement Of Airway Functionmentioning

confidence: 99%

Effects of salbutamol and enantiomers on allergen-induced asthmatic reactions and airway hyperreactivity

Westerhof

Zuidhof²,

Kok³

et al. 2005

European Respiratory Journal

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Salbutamol consists of a racemic mixture of R-and S-salbutamol. R-salbutamol (levalbuterol) is the active bronchodilating enantiomer, whereas S-salbutamol is thought to be pharmacologically inactive or to exert adverse effects.This study evaluated the bronchoprotective effects of inhalation of therapeutically relevant doses of the racemate and individual enantiomers in guinea pigs.It was found that basal airway reactivity to histamine was similarly reduced 30 min after inhalation of equivalent doses of RS-and R-salbutamol; this protective effect disappeared within 3 h. Inhalation of RS-and R-salbutamol 30 min before and 5.5 h after allergen challenge suppressed allergen-induced airway hyperreactivity to histamine after the early and late asthmatic reaction, completely inhibiting the early asthmatic reaction and tending to reduce the development of the late asthmatic reaction. At 5 h after allergen challenge, the inhibition of airway hyperreactivity was more pronounced in animals treated with R-salbutamol compared to racemate-treated animals. Both basal airway reactivity and allergen-induced hyperreactivity were not affected by S-salbutamol. Inflammatory cell infiltration was not affected by the racemate or the individual enantiomers.In conclusion, inhalation of therapeutically relevant doses of R-and RS-salbutamol effectively suppress allergen-induced airway reactivity after the early and late asthmatic reactions, the Renantiomer being slightly more potent with respect to early airway reactivity than the racemate. No adverse effects were observed for the S-enantiomer.

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Summative interaction between astaxanthin, Ginkgo biloba extract (EGb761) and vitamin C in Suppression of respiratory inflammation: a comparison with ibuprofen

Haines

Varga

Bak

et al. 2010

Phytotherapy Research

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In this study, combinations of Ginkgo biloba leaf extract (EGb761) plus the carotenoid antioxidant astaxanthin (ASX) and vitamin C were evaluated for a summative dose effect in the inhibition of asthma‐associated inflammation in asthmatic guinea‐pigs. Ovalbumin‐sensitized Hartley guinea‐pigs challenged with ovalbumin aerosol to induce asthma, were administered EGb761, ASX, vitamin C or ibuprofen. Following killing, bronchoalveolar lavage (BAL) fluid was evaluated for inflammatory cell infiltrates and lung tissue cyclic nucleotide content. Each parameter measured was significantly altered to a greater degree by drug combinations, than by each component acting independently. An optimal combination was identified that included astaxanthin (10 mg/kg), vitamin C (200 mg/kg) and EGb761 (10 mg/kg), resulting in counts of eosinophils and neutrophils each 1.6‐fold lower; macrophages 1.8‐fold lower, cAMP 1.4‐fold higher; and cGMP 2.04‐fold higher than levels in untreated, asthmatic animals (p < 0.05). In conclusion, EGb761, ASX and vitamin C are shown here to interact summatively to suppress inflammation with efficacy equal to or better than ibuprofen, a widely used non‐steroidal antiinflammatory drug (NSAID). Such combinations of non‐toxic phytochemicals constitute powerful tools for the prevention of onset of acute and chronic inflammatory disease if consumed regularly by healthy individuals; and may also augment the effectiveness of therapy for those with established illness. Copyright © 2010 John Wiley & Sons, Ltd.

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A novel method to assess airway function parameters in chronically instrumented, unrestrained guinea-pigs

Cited by 47 publications

References 20 publications

Bronchoprotection by Olodaterol Is Synergistically Enhanced by Tiotropium in a Guinea Pig Model of Allergic Asthma

Bronchoprotection by Olodaterol Is Synergistically Enhanced by Tiotropium in a Guinea Pig Model of Allergic Asthma

Effects of salbutamol and enantiomers on allergen-induced asthmatic reactions and airway hyperreactivity

Summative interaction between astaxanthin, Ginkgo biloba extract (EGb761) and vitamin C in Suppression of respiratory inflammation: a comparison with ibuprofen

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