A novel method to assess gastric accommodation and peristaltic motility in conscious rats

Janssen, Pieter; Nielsen, Maria Astin; Hirsch, I; Svensson, David; Gillberg, Per‐Göran; Hultin, Leif

doi:10.1080/00365520701580066

Cited by 18 publications

(34 citation statements)

References 31 publications

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“…This increase was followed by a plateau phase during which IGP hardly increased despite further nutrient infusion. These findings correspond to results from previous experiments in which the same animal model was used (15). It was suggested that the plateau phase, during which IGP is stable but intragastric nutrient infusion continues, represents a reflex gastric relaxation upon food ingestion and thus GA.…”

Section: Discussionsupporting

confidence: 77%

“…The experimental protocol was similar to that used in Janssen et al (15). Animals were fasted overnight before the beginning of each experiment to prevent the presence of food particles in the stomach, and access to water was provided ad libitum.…”

Section: Ga Studymentioning

confidence: 99%

“…FISTULA IMPLANTATION. Gastric fistulas were chronically implanted in all animals at least 2 wk before the start of the experiments, as previously described by Janssen et al (15). In short, rats were anesthetized using inhalation anesthesia (2.5% Isoflurane).…”

Section: Ga Studymentioning

confidence: 99%

“…We recently described a method to assess GA in rats by measuring the IGP during intragastric infusion of a liquid test meal (15). During infusion of a test meal, IGP increased initially until an inflection point was reached, after which the IGP stabilized despite further gastric distension.…”

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confidence: 99%

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Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Verschueren

Janssen

Oudenhove

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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Verschueren S, Janssen P, Van Oudenhove L, Hultin L, Tack J. Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats. Am J Physiol Gastrointest Liver Physiol 307: G122-G128, 2014. First published April 17, 2014 doi:10.1152/ajpgi.00043.2014 is an anorexigenic hormone released from pancreatic F cells upon food intake. We aimed to determine the effect of PP on gastric accommodation and gastric emptying in conscious Wistar HAN rats to investigate whether effects on motor function could contribute to its anorexigenic effects. Intragastric pressure (IGP) was measured through a chronically implanted gastric fistula during the infusion of a nutrient meal (Nutridrink; 0.5 ml/min). Rats were treated with PP (0, 33 and 100 pmol·kg Ϫ1 ·min Ϫ1 ) in combination with N G -nitro-L-arginine methyl ester (L-NAME; 180 mg·kg Ϫ1 ·h Ϫ1 ), atropine (3 mg·kg Ϫ1 ·h Ϫ1 ), or vehicle. Furthermore, the effect of PP was tested after subdiaphragmal vagotomy of the stomach. Gastric emptying of a noncaloric and a caloric meal after treatment with 100 pmol·kg Ϫ1 ·min Ϫ1 PP or vehicle was compared using X-rays. PP significantly increased IGP during nutrient infusion compared with vehicle (P Ͻ 0.01). L-NAME and atropine significantly increased IGP during nutrient infusion compared with vehicle treatment (P Ͻ 0.005 and 0.01, respectively). The effect of PP on IGP during nutrient infusion was abolished in the presence of L-NAME and in the presence of atropine. In vagotomized rats, PP increased IGP compared with intact controls (P Ͻ 0.05). PP significantly delayed gastric emptying of both a noncaloric (P Ͻ 0.05) and a caloric (P Ͻ 0.005) meal. PP inhibits gastric accommodation and delays gastric emptying, probably through inhibition of nitric oxide release. These results indicate that, besides the well-known centrally mediated effects, PP might decrease food intake through peripheral mechanisms. gastric pressure; nutrient tolerance; nitric oxide; vagus nerve BETWEEN MEALS, GASTRIC SMOOTH muscle maintains a high resting tone because of the myoelectrical properties of the smooth muscle but also because of a constant cholinergic input from the vagal nerves. The stomach is known to relax upon food intake. This reflex relaxation, also referred to as gastric accommodation (GA), enables the stomach to receive large quantities of food without an increase in intragastric pressure (IGP) (35). Relaxation of the stomach upon food intake is mediated via vago-vagal reflex pathways, eventually leading to activation of mainly nitrergic nerves in the enteric nervous system (38). Nitric oxide (NO) finally relaxes the smooth muscle cells, and the gastric tone decreases (19).We recently described a method to assess GA in rats by measuring the IGP during intragastric infusion of a liquid test meal (15). During infusion of a test meal, IGP increased initially until an inflection point was reached, after which the IGP stabilized despite further gastric distension. It was suggested that this inflection point represents the onset of...

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Section: Discussionsupporting

confidence: 77%

Section: Ga Studymentioning

confidence: 99%

Section: Ga Studymentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Verschueren

Janssen

Oudenhove

et al. 2014

American Journal of Physiology-Gastrointestinal and Liver Physi

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“…This finding shows that the adaptive relaxation of the stomach is induced through the generation of NO. Janssen et al (2008Janssen et al ( , 2010 showed that L-NAME significantly increased intragastric pressure during stomach distension, indicating decreased gastric accommodation. These reports are in accord with our present result with L-NAME.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of adaptive relaxation of the rat stomach using an orally inserted balloon instead of surgical intervention by demonstrating the effects of capsaicin and <i>N</i><sup>ω</sup>-nitro-L-arginine methylester

Uchida

Shimizu

2012

J. Smooth Muscle Res.

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Gastric functions such as adaptive relaxation have usually been monitored in rats using a surgically inserted barostat's balloon. However, surgery causes physiological damage to the rat stomach. This study is an investigation of adaptive relaxation of the rat stomach using a slightly modified balloon, which is introduced into the stomach through the mouth of anesthetized rats without the need for balloon surgery, attached to a brostat. In this case, the balloon was placed between the fore-stomach and the fundus, but towards the fore-stomach. The balloon volume increased gradually just after an increment in the balloon pressure, and reached a plateau within 1 min. This increased volume just after the increment of the balloon pressure was defined as adaptive relaxation. Adaptive relaxation increased with pressure increases in a pressure dependent manner. Pre-treatment with N ω -nitro-L-arginine methylester (30 mg/kg, i.v.) caused this adaptive relaxation to be significantly inhibited as compared with the control. On the contrary, adaptive relaxation was significantly enhanced by pre-treatment with capsaicin (0.5 mg/kg, p.o.). These findings show that this method is both useful for investigating the physiology of adaptive relaxation of the stomach without surgery and to show that nitric oxide plays an important role in the adaptive relaxation of the stomach as reported previously.

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A Novel Method for Study of Gastric Mechanical Functions in Conscious Mice

et al. 2008

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A novel method has been developed for simultaneous study of gastric emptying, antral motility, and gastric muscle tone in conscious mice. Intragastric pressure was measured during infusion of an X-ray-opaque, viscous meal through a chronically implanted gastric fistula (0.25 ml/min). Compared with vehicle treatment, molsidomine (nitric oxide donor) and atropine (muscarinic receptor antagonist) treatment significantly reduced the area under the intragastric pressure curve (AUC) by 37 +/- 4% and 35 +/- 3%, respectively, (mean +/- S.E.M.) whereas N (G)-nitro-L-arginine methyl ester (L-NAME; nitric oxide synthase inhibitor) significantly increased the AUC by 20 +/- 3%. Atropine also significantly reduced the frequency and amplitude of stomach contraction-induced intragastric pressure waves while molsidomine only reduced the frequency. Gastric emptying, as assessed by X-ray imaging, was significantly delayed after L-NAME and atropine treatment. This methodology is the first to enable simultaneous assessment of gastric emptying, antral motility, and gastric tone in conscious mice and confirmed the important role of nitrergic and cholinergic innervation.

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A novel method to assess gastric accommodation and peristaltic motility in conscious rats

Abstract: We conclude that IGP measurement during test-meal infusion represents an efficient and novel method to compare gastric accommodation and peristaltic motility in the whole stomach of conscious rats.

Cited by 18 publications

References 31 publications

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Effect of pancreatic polypeptide on gastric accommodation and gastric emptying in conscious rats

Evaluation of adaptive relaxation of the rat stomach using an orally inserted balloon instead of surgical intervention by demonstrating the effects of capsaicin and <i>N</i><sup>ω</sup>-nitro-L-arginine methylester

A Novel Method for Study of Gastric Mechanical Functions in Conscious Mice

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