1986
DOI: 10.1002/bit.260280404
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A novel method of determination of the internal enzyme distribution within porous solid supports and the deactivation rate constant

Abstract: This article presents a method for determining the rate constant for deactivation and the internal distribution of immobilized enzyme. This method makes use of the parallel deactivation process in a diffusion-controlled regime, in which the internal activity profile behaves like a penetration front. This front basically traces through the initial active enzymatic profile, and one can determine the internal profile and the rate constant for deactivation from the experimentally observable bulk concentration vers… Show more

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Cited by 13 publications
(2 citation statements)
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References 23 publications
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“…In several papers the intraparticle biocatalytic distribution was fitted from macroscopic data of systems with relatively straightforward kinetics (21)(22)(23)(24)(25). To the best of our knowledge, up to now little or no research has focused on the modeling of the intraparticle distribution in systems with complex kinetics under non-steady-state conditions.…”
Section: Introductionmentioning
confidence: 99%
“…In several papers the intraparticle biocatalytic distribution was fitted from macroscopic data of systems with relatively straightforward kinetics (21)(22)(23)(24)(25). To the best of our knowledge, up to now little or no research has focused on the modeling of the intraparticle distribution in systems with complex kinetics under non-steady-state conditions.…”
Section: Introductionmentioning
confidence: 99%
“…Van der Wielen (1994) modeled the enzymatic deacylation of penicillin G into phenylacetic acid and 6-aminopenicillanic acid taking coupled electrostatic transport of ionic species into account. The influence of heterogeneous enzyme distributions in systems with nonionic reactants and with relatively simple enzyme kinetics (zeroth order, first order, and MichaelisMenten) was investigated by Do and Hossain (1986) and Hossain and Do (1989), who fitted some extreme intraparticle enzyme distributions to macroscopic data sets.…”
Section: Introductionmentioning
confidence: 99%