A Novel Method for the Preparation of Template DNA for PCR from Beer to Detect Materials and to Develop DNA Markers to Evaluate the Quality of Beer

Nakamura, Sumiko; Tsushima, Ryosuke; Ohtsubo, Ken’ichi

doi:10.1271/bbb.120969

Cited by 6 publications

(2 citation statements)

References 47 publications

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“…Thus, it is essential to utilize effective methods of extracting DNA that can be used for qPCR or other nucleic acid amplification methods directly from beer samples. An example of a relatively typical method described by Nakamura et al (2013) , employs the use of lyophilization and magnetic beads.…”

Section: Methods Of Bsm Detectionmentioning

confidence: 99%

Methods for detection and identification of beer-spoilage microbes

Oldham

Held

2023

Front. Microbiol.

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It is critical that breweries of all sizes routinely monitor the microbiome of their process to limit financial losses due to microbial contamination. Contamination by beer-spoiling microbes (BSMs) at any point during the brewing process may lead to significant losses for breweries if gone undetected and allowed to spread. Testing and detection of BSMs must be routine and rapid, and because even small breweries need the capability of BSM detection and identification, the method also needs to be affordable. Lactic acid bacteria (LAB) are responsible for most spoilage incidents, many of which have been shown to enter the viable but nonculturable (VBNC) state under conditions present in beer such as cold or oxidative stress. These bacteria are invisible to traditional methods of detection using selective media. This article describes several methods of BSM detection and identification that may be useful in the majority of craft breweries. While there are several genomic methods that meet some or many qualifications of being useful in craft breweries, real-time quantitative polymerase chain reaction (qPCR) currently best meets the desired method characteristics and holds the most utility in this industry, specifically SYBR Green qPCR. qPCR is a targeted method of detection and identification of microbes that is affordable, rapid, specific, sensitive, quantitative, and reliable, and when paired with valid DNA extraction techniques can be used to detect BSMs, including those in the VBNC state.

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Section: Methods Of Bsm Detectionmentioning

confidence: 99%

Methods for detection and identification of beer-spoilage microbes

Oldham

Held

2023

Front. Microbiol.

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“…Techniques to sample airborne DNA exist 23 . Furthermore, other protocols could also be used to catalogue plant DNA 24 , such as malt and hop varieties, and to map the bacterial diversity.…”

Section: Discussion and Future Perspectivesmentioning

confidence: 99%

BeerDeCoded: the open beer metagenome project

Sobel¹,

Henry²,

Rotman³

et al. 2017

F1000Res

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Next generation sequencing has radically changed research in the life sciences, in both academic and corporate laboratories. The potential impact is tremendous, yet a majority of citizens have little or no understanding of the technological and ethical aspects of this widespread adoption. We designed BeerDeCoded as a pretext to discuss the societal issues related to genomic and metagenomic data with fellow citizens, while advancing scientific knowledge of the most popular beverage of all. In the spirit of citizen science, sample collection and DNA extraction were carried out with the participation of non-scientists in the community laboratory of Hackuarium, a not-for-profit organisation that supports unconventional research and promotes the public understanding of science. The dataset presented herein contains the targeted metagenomic profile of 39 bottled beers from 5 countries, based on internal transcribed spacer (ITS) sequencing of fungal species. A preliminary analysis reveals the presence of a large diversity of wild yeast species in commercial brews. With this project, we demonstrate that coupling simple laboratory procedures that can be carried out in a non-professional environment with state-of-the-art sequencing technologies and targeted metagenomic analyses, can lead to the detection and identification of the microbial content in bottled beer.

show abstract

BeerDeCoded: the open beer metagenome project

Sobel¹,

Henry²,

Rotman³

et al. 2017

F1000Res

View full text Add to dashboard Cite

Next generation sequencing has radically changed research in the life sciences, in both academic and corporate laboratories. The potential impact is tremendous, yet a majority of citizens have little or no understanding of the technological and ethical aspects of this widespread adoption. We designed BeerDeCoded as a pretext to discuss the societal issues related to genomic and metagenomic data with fellow citizens, while advancing scientific knowledge of the most popular beverage of all. In the spirit of citizen science, sample collection and DNA extraction were carried out with the participation of non-scientists in the community laboratory of Hackuarium, a not-for-profit organisation that supports unconventional research and promotes the public understanding of science. The dataset presented herein contains the targeted metagenomic profile of 39 bottled beers from 5 countries, based on internal transcribed spacer (ITS) sequencing of fungal species. A preliminary analysis reveals the presence of a large diversity of wild yeast species in commercial brews. With this project, we demonstrate that coupling simple laboratory procedures that can be carried out in a non-professional environment, with state-of-the-art sequencing technologies and targeted metagenomic analyses, can lead to the detection and identification of the microbial content in bottled beer. Competing interests: GR is the co-founder, CTO and a shareholder of SwissDeCode, a company selling point-of-need DNA tests to food manufacturers for food safety and compliance. JS, LH and NR declare no competing interests.

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A Novel Method for the Preparation of Template DNA for PCR from Beer to Detect Materials and to Develop DNA Markers to Evaluate the Quality of Beer

Cited by 6 publications

References 47 publications

Methods for detection and identification of beer-spoilage microbes

Methods for detection and identification of beer-spoilage microbes

BeerDeCoded: the open beer metagenome project

BeerDeCoded: the open beer metagenome project

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