A novel method for the spectrophotometric determination of curcumin and its application to curcumin spices

Jasim, Fadhil; Ali, Fatima

doi:10.1016/0026-265x(88)90008-2

Cited by 35 publications

(18 citation statements)

References 4 publications

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“…It has been reported that curcumin has many pharmacological functions, such as antioxygenation, antibiosis and antitumor [1][2][3] . Methods such as high-performance thin-layer chromatographic (HPTLC) [4][5][6][7] , high-performance liquid chromatography (HPLC) [8][9][10][11] , capillary electrophoresis with amperometric detection (CE-AD) 12 , matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOFMS) 13 , spectrofluorimetry 14,15 , liquid phase tandem mass spectrometry (LC-MS/MS) 16,17 , 1 H NMR 17 , resonance light scattering technique (RLS) 18 and spectrophotometry [19][20][21] have been employed to determine curcumin in a variety of matrixes. However, most of these methods have low sensitivity, need a large number of organic solvents or metal ions.…”

Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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A new ionic liquid (IL) based micro-extraction method was developed for the spectrophotometry and spectrofluorometry determination of curcumin in curry products. ILs can effectively extract curcumin from aqueous solution which were used instead of large-scale organic solvents to reduce environmental pollution and production cost. Results show that the fluorescence of curcumin is considerably enhanced in 1-hexyl-3-methylimidazolium hexafluorophosphate ([Hmim][PF 6 ]). Under optimized conditions, the absorbance of curcumin is proportional to the concentration of curcumin in the range of 18.4 ng/mL to 494 ng/mL, and the detection limit is 6.1 ng/mL. In addition, the fluorescence intensity of curcumin is proportional to the concentration of curcumin in the range of 0.050 ng/mL to 120.1 ng/mL, and the detection limit is 0.002 ng/mL. Therefore, an efficient spectrophotometry and spectrofluorometry method for the determination of curcumin was established, and the actual concentration in curry products was satisfactorily determined. The content of curcumin in curry sauce were 14.10±0.40 and 14.32±0.37 mg/g when using the spectrophotometry and spectrofluorometry, the content of curcumin in curry powder were 2.50±1.73 and 2.75±1.40 mg/g when using the spectrophotometry and spectrofluorometry.

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Section: Curcuminmentioning

confidence: 99%

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Chang

Chen

et al. 2014

J. Chil. Chem. Soc.

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“…The enol form is the most energetically stable, and its most common commercial form have a molecular weight of 368.38 g.mol −1 . The term curcumin may refer to a mixture of three curcuminoids: curcumin, desmethoxycurcumin and bis-desmethoxycurcumin, with different number of hydrogen atoms substituting the -OCH 3 radical [1,2].…”

Section: Introductionmentioning

confidence: 99%

Validation of Photodynamic Action via Photobleaching of a New Curcumin-Based Composite with Enhanced Water Solubility

et al. 2014

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Motivated by the photochemical and photophysical properties of curcumin-based composites, the characteristics of a new curcumin-based water-soluble salt were investigated via absorption and fluorescence spectroscopy. Photobleaching was investigated using a set of LEDs in three different wavelengths (405 nm, 450 nm and 470 nm) to illuminate an aqueous solution of curcumin, evaluating its degradation for five different exposure times (0, 5, 15, 45 and 105 minutes). The results were compared with equivalent measurements of dark degradation and illumination in the presence of a singletoxygen quencher. Three solution concentrations (50, 100 and 150 μg/ml) were studied. To measure the fluorescence, it was used low power 405 nm excitation laser source. Time dependent photodegradation of curcumin was observed, as compared to the natural degradation of samples maintained on a dark environment. Two main absorption peaks were detected and their relation responded to both concentration and wavelength of the illumination source. A spectral correlation between absorption of curcumin and the emission bands of the sources showed an optimal spectral overlap for the 450 nm LED. For this source, photobleaching showed a less intense degradation on the presence of singlet oxygen quencher. This last result confirmed singlet oxygen production in vitro, indicating a strong potential of this composite to be used as a bluelight-activated photosensitizer.

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“…Therefore, the investigation for the determination of CU is important for clinical medicine and pharmacology [1][2][3][4]. Methods such as thinlayer chromatography [5][6][7], high performance liquid chromatography [8][9][10][11], spectrofluorimetry [12][13][14][15] and UV-Vis spectrophotometry [16,17] have been applied to determine CU in a variety of matrices such as curcuma longa, food stuffs and biological materials. CU has extensive absorption around 424 nm and can emit the fluorescence around 495 nm in organic solvent, but they decrease strongly in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence enhancement effect for the determination of curcumin with yttrium(III)—curcumin—sodium dodecyl benzene sulfonate system

Wang

Huang

Wang

2008

Journal of Luminescence

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A novel method for the spectrophotometric determination of curcumin and its application to curcumin spices

Cited by 35 publications

References 4 publications

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

High Sensitivity Method for Determination of Trace Curcumin in the Aqueous Phase

Validation of Photodynamic Action via Photobleaching of a New Curcumin-Based Composite with Enhanced Water Solubility

Fluorescence enhancement effect for the determination of curcumin with yttrium(III)—curcumin—sodium dodecyl benzene sulfonate system

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