2018
DOI: 10.1093/conphys/coy008
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A novel method for the measurement of glucocorticoids in dermal secretions of amphibians

Abstract: We are developing a novel method using non-invasive skin swabs to measure stress in several amphibians from terrestrial, semi-aquatic and fully aquatic species both at the zoo and in the wild. Upon further testing, this technique will allow future studies to understand how the environment is impacting amphibian health and susceptibility to disease.

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Cited by 30 publications
(60 citation statements)
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“…Noninvasive sampling also allows for reduced sample sizes to estimate temporal patterns and control for individual variation in marked individuals or individually-recognizable individuals in the lab and wild. Hormone metabolites can be quantified through a variety of non-invasive biological samples, such as urine, water, skin swabs, and saliva, and have been used with success to monitor amphibian endocrine functions (Gabor et al, 2013;Narayan, 2013;Hammond et al, 2018;Santymire et al, 2018; Table 1). Measurement of adrenal GC metabolites in urine and feces is of considerable interest to conservation managers as it gives an indication of the link between stress and reproductive success in animals (Carroll et al, 1990;Creel et al, 1997;Wasser et al, 1997;Hopkins et al, 1999;Holt et al, 2003;Pukazhenthi and Wildt, 2004).…”
Section: Using Non-invasive Methods Vs Plasma or Whole-body Homogenamentioning
confidence: 99%
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“…Noninvasive sampling also allows for reduced sample sizes to estimate temporal patterns and control for individual variation in marked individuals or individually-recognizable individuals in the lab and wild. Hormone metabolites can be quantified through a variety of non-invasive biological samples, such as urine, water, skin swabs, and saliva, and have been used with success to monitor amphibian endocrine functions (Gabor et al, 2013;Narayan, 2013;Hammond et al, 2018;Santymire et al, 2018; Table 1). Measurement of adrenal GC metabolites in urine and feces is of considerable interest to conservation managers as it gives an indication of the link between stress and reproductive success in animals (Carroll et al, 1990;Creel et al, 1997;Wasser et al, 1997;Hopkins et al, 1999;Holt et al, 2003;Pukazhenthi and Wildt, 2004).…”
Section: Using Non-invasive Methods Vs Plasma or Whole-body Homogenamentioning
confidence: 99%
“…In this table, we do not evaluate the non-invasive dermal swab method because the GCs detected in skin secretions could be independent of the HPI axis as the skin itself is recognized as an endocrine tissue. GC responses in dermal skin secretions are possibly a result of a much slower rate of GC secretion than GC metabolites detected via other non-invasive samples, such as water or urine (Santymire et al, 2018).…”
Section: Using Non-invasive Methods Vs Plasma or Whole-body Homogenamentioning
confidence: 99%
“…We examined the GC response of salamanders to elevated temperature using a water-borne CORT assay (Gabor et al, 2013) which provides an integrated measure reflecting an average of blood GCs that have been metabolized and excreted from urine and feces, and possibly through the skin, over a period of cumulative exposure (Santymire et al, 2018;Sheriff et al, 2011). We measured CORT release rates for each salamander at an average temperature (15°C) and an elevated temperature (25°C).…”
Section: Thermal Cort Response Experimentsmentioning
confidence: 99%
“…Metrics of stress physiology are among the most commonly used tools in conservation physiology, and GCs in particular continue to amass popularity as indicators of disturbance in wildlife ( Dantzer et al , 2014 ; Lennox and Cooke, 2014 ; Sopinka et al , 2015 ). The desire to ascertain physiological stress through GC concentrations is evidenced by the array of novel sample types continually being investigated, which has recently extended to whale ear wax ( Trumble et al , 2013 ) and amphibian skin secretions ( Santymire et al , 2018 ). If a sample type could potentially contain GCs, researchers are working to collect, process and assay it as effectively as possible, and we applaud the ingenuity involved.…”
Section: Next Steps: Continuing To Improve the Value Of The Toolboxmentioning
confidence: 99%