A novel method for the preconcentration and determination of ampicillin using electromembrane microextraction followed by high‐performance liquid chromatography

Aghaei, Ali; Jazi, Mehdi Erfani; Mlsna, Todd; Kamyabi, Mohammad Ali

doi:10.1002/jssc.201900016

Cited by 23 publications

(3 citation statements)

References 31 publications

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“…In the context of EPs and milk, Aghaei et al used EME for the extraction and preconcentration of ampicillin antibiotic residues in cow milk samples prior to their analysis using HPLC-UV [ 94 ]. The EME procedures involved the optimization of SLM composition, which were mainly composed of octan-1-ol, reduced graphene oxide, and silver nanoparticles.…”

Section: Extraction Of Eps From Milkmentioning

confidence: 99%

Recent Advances in the Chromatographic Analysis of Emerging Pollutants in Dairy Milk: A Review (2018–2023)

Ashraf,

Morsi,

Usman

et al. 2024

Molecules

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Emerging pollutants (EPs) encompass natural or synthetic substances found in the environment that pose potential risks, but which have only recently been recognized or monitored. EPs consist of various categories, including pesticides, pharmaceuticals, hormones, mycotoxins, and endocrine-disrupting chemicals (EDCs). Through several pathways, EPs can access food, potentially leading to health impacts when safe concentrations are exceeded. Milk, being a highly nutritious food product that is heavily consumed by many consumers of different ages, is a crucial food matrix where EPs should be regularly monitored. In the literature, a large number of studies have been dedicated to the determination of different EPs in dairy milk, employing different analytical techniques to do so. Chromatography-based techniques are the most prevalent means used for the analysis of EPs in milk, demonstrating significant efficiency, sensitivity, and accuracy for this specific purpose. The extraction of EPs from a complex matrix like milk is essential prior to performing chromatographic analysis. This review comprehensively covers relevant research papers on the extraction and subsequent detection and determination of EPs in milk using chromatographic methods from 2018 to 2023.

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Section: Extraction Of Eps From Milkmentioning

confidence: 99%

Recent Advances in the Chromatographic Analysis of Emerging Pollutants in Dairy Milk: A Review (2018–2023)

Ashraf,

Morsi,

Usman

et al. 2024

Molecules

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“…One of the types of modified C 18 columns is polarendcapped stationary phase (Fig. 2) where an addition of a polar functional group [19][20][21] enables a full interaction with the longer alkyl chains and makes the retention of polar analytes under highly aqueous conditions more reproducible [1,22,23]. The C 18 ligand provides hydrophobic interactions, while the polar-modified surface provides increased retention of polar substances.…”

Section: Introductionmentioning

confidence: 99%

Separation of ampicillin on polar-endcapped phase: Development of the HPLC method to achieve its correct dosage in cardiac surgery

Kubíčková

Rácová

Strojil

et al. 2023

AChrom

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The accurate, simple, and selective reversed phase high performance liquid chromatography (RP-HPLC) has been established and validated for the determination of an antibiotic ampicillin (AMP) in human blood plasma. The SPE extraction was used for the sample preparation. Chromatographic separation was accomplished by a mobile phase containing 15 mM monopotassium phosphate solution of pH 3.3 and methanol (75:25, v/v) in an isocratic mode at a flow rate of 1.4 mL min−1 at 30 °C. The separation was evaluated on a column with a new polar-endcapped C18 stationary phase Arion® Polar C18 or well-known phase Luna® Omega Polar C18. Excellent linearity (R 2 0.9998) was shown over range 10–300 mg L−1 with mean percentage recovery 90%. Peak shapes were symmetrical in both columns, Arion® Polar C18 and Luna® Omega Polar C18, with asymmetry factor of 1.0 and 1.4, tailing factor of 1.0 and 1.2, and retention factor of 4.6 and 5.6, respectively. The Arion® Polar C18 was almost 1.4-fold more effective than Luna® Omega Polar C18 phase. The LOQ for ampicillin was achieved 10 mg L−1 for Luna® Omega Polar C18 and 5 mg L−1 for Arion® Polar C18 using 20 µL of a solution containing 0.24 mg mL−1 of cephalexin as an internal standard. A number of articles dealing with the determination of ampicillin is limited, therefore, this study showed the HPLC method suitable for the determination of AMP in human blood plasma from patient who underwent elective cardiac surgical revascularization. In addition, the determination of AMP was also performed for the first time using an Arion® Polar C18 column, which effectively separated AMP from other compounds present in human blood plasma. This new polar-endcapped phase can help in separation of polar antibiotics or other polar compounds, which are unsuccessfully separated on conventional C18 column, and thus can help during method development.

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“…Additionally, the polarity and magnitude of the electric field add selectivity to the system. EME has, thus, been used for selective extraction of a wide range of both basic, acidic, and zwitterionic substances including pharmaceutical drugs [2–5], peptides [6–7], heavy metals [8–10], and salts [11]. In recent years, the application of EME for extraction of polar and even highly polar analytes has also gained interest.…”

Section: Introductionmentioning

confidence: 99%

Selectivity and efficiency of electromembrane extraction of polar bases with different liquid membranes—Link to analyte properties

Hansen

Tirandaz

Pedersen‐Bjergaard

2021

J of Separation Science

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In the present fundamental study, selectivity and efficiency of electromembrane extraction of 50 polar basic substances (−6.7 < log P < +1.0) was systematically studied for ten different supported liquid membranes. For each model substance, 23 molecular descriptors were collected and these were investigated as potential parameters for understanding of extraction efficiency and selectivity by means of partial least squares regression. Overall, a highly aromatic deep eutectic solvent composed of coumarin and thymol with addition of 2% ionic carrier (di(2‐ethylhexyl) phosphate) provided the highest extraction efficiency with an average extraction yield of 69% from pure water samples, 55% from plasma, and 62% from urine. With this solvent system, ionic, cation‐π, and π‐π interactions between the supported liquid membrane and analytes were dominant. Supported liquid membranes without aromaticity, however, operated primarily based on hydrogen‐bonding interactions. This is the first time the relationship between analyte properties, solvent composition, and extraction yield has systematically been studied for polar bases in electromembrane extraction. This new knowledge represents a first step toward enabling future development and optimization of electromembrane extraction systems for polar bases based on rational design, rather than trial‐and‐error approaches.

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A novel method for the preconcentration and determination of ampicillin using electromembrane microextraction followed by high‐performance liquid chromatography

Cited by 23 publications

References 31 publications

Recent Advances in the Chromatographic Analysis of Emerging Pollutants in Dairy Milk: A Review (2018–2023)

Recent Advances in the Chromatographic Analysis of Emerging Pollutants in Dairy Milk: A Review (2018–2023)

Separation of ampicillin on polar-endcapped phase: Development of the HPLC method to achieve its correct dosage in cardiac surgery

Selectivity and efficiency of electromembrane extraction of polar bases with different liquid membranes—Link to analyte properties

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